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EN
The paper concerns a very important element of innate immunity, i.e. the polimorfonuclear cell, which by the process of phagocytosis participates in defending the macroorganism against pathogens. New facts about phagocytosis have been described - metalloproteises and galectins regulating migration, selectins enhancing adherence, opsonins taking part in absorbance and oxygen dependent and independent systems as mechanisms of intracellular digestion and killing. Moreover, fate of the phagocyted polimorfonuclear cell has been shown, including apoptosis.
PL
Oceniano aktywność fagocytarną związaną z wybuchem tlenowym granulocytów obojętnochłonnych u chorych z boreliozą z Lyme. Wykazano spadek wartości badanych parametrów w ostrej fazie choroby oraz ich normalizację w badaniu odległym, wykonanym po sześciu miesiącach od zakończenia leczenia. Poprawa badanych funkcji neutrofilów w badaniach odległych sugeruje, że obserwowane zmiany mają charakter nabyty, uwarunkowany obecnością krętka Borrelia burgdorferi.
EN
In recent years in Poland, the interest has increased in studies about tick borne diseases, mainly Lyme borreliosis. Immune response and genotype of pathogen play an important role in the course of this disease. Phagocytic cells, especially PMN are dominant in defence mechanisms against bacterial infections. The main feature of PMN is their ability to destroy pathogenic microorganisms by phagocytosis. The aim of this study was to estimate the phagocytic activity of PMN connected with intracellular respiratory burst in patients with Lyme borreliosis. The PMN activity tests completed were: phagocytosis, spontaneous and reducted of nitrotetralizate blue test (NBT). Decreased phagocytic activity and oxygen metabolism of PMN from patients with borreliosis in comparison with values of controls were found. Normalization of these parameters after treatment was observed. Changed phagocytic activity connected with intracellular oxygen metabolism during the course of therapy was the main observation. Depression of phagocytic activity of PMN connected with oxygen metabolism can influence defence reactions in patients with Lyme borreliosis. It is suggested that changes observed are acquired and associated with Borrelia burgdorferi presence.
EN
Metabolic activity of neutrophilic granulocytes of carp (Cyprinus carpio L.) aged from 2 months - 5 years determined by the Nitrotetrazolium blue reduction test (NBT) has been rendered as the percentage of NBT⁺ cells, NBT index and quantity of formazane (g/l). A decrease of the percentage of NBT⁺ cells from the 5th months and the NBT index from the 10 month of the carps lives was discovered. The highest percentage of the NBT⁺ cells and NBT index was found in carps 2 months old, the lowest in the carps at the age of 5 years and 25 months, respectively. Sex did not affect these parameters. Age and sex of the carp did not affect the quantity of formazane in the blood.
EN
The purpose of the study was to assess the condition of peripheral blood neutrophiles in horses through using the luminal-enhanced chemiluminescence (CL) method. The study population consisted of 33 clinically healthy horses of both sexes (aged 3-15 years). The chemiluminescence of non-stimulated neutrophiles was measured as well as of those stimulated by a receptor stimulus - myosin (Z), opsonic zymosan (OZ) and formyl-Met-Leu-Phe (fMLP) and an extrareceptor stimulus - phorbol myristate acete (PMA). The results of the study demonstrated that the chemiluminescence of neutrophiles stimulated by Z and OZ was considerably higher in comparison to the values of CL stimulated by fMLP and PMA. The use of stimulators can trigger a "respiratory burst" and may be helpful in assessing the phagocyte activity of equine neutrophiles.
EN
The aim of the study was to estimate the effect of tylosin and prebiotics (β-glucans and mannanooligosaccharides) added to feed on the phagocitc activity of polimorfonuclear leukocytes (PMNL) in calves. The study was performed on 36 clinically healthy, Black and White Lowland calves, aged 6-8 weeks. The animals were randomly divided into three equal groups. Calves in group I were fed a feedingstuff supplemented with tylosin. Group II received prebiotics, and group III (control) was fed the same feedingstuff without additives. The following phagocytic parameters were determined in the peripheral blood of the calves: phagocytic index (IF), the percentages of phagocytic (%kf) and NBT-positive cells, and the spontaneous migration of neutrophils (MG). The results showed that both prebiotics and tylosin caused a significant increase in the phagocytic activity of PMNL in the experimental groups. This was expressed by the higher values of the examined phagocytic parameters compared with the controls.
EN
The studies were done on 50 male Wistar rats divided into five groups: 1 - controls, II - rats immobilized for 13 hr, III - animals immobilized after peritoneal infection with a pathogenic E. coli, IV - rats immobilized after the application of vitamin E (30 mg/kg bw) for six continous days, V - rats immobilized after the application of vitamin E and peritoneal injection of a pathogenic E. coli. Metabolic neutrophil activity (NBT-test) was determined spectrophotometrically in blood and the level of corticosterone was estimated in blood plasma by the radiocompetition method. It was found that an increase of corticosterone and decrease of metabolic neutrophil activity appeared in rats after immobilization (group II). However, a significant decrease of neutrophil activity and an inconsiderable increase of corticosterone were noted in group III and V. Immobilization of rats after the administration of witamin E did not affect the level of the examined parameters (group IV).
EN
The examinations were done on 7 healthy Polish ponies from 1.5 to 14 years of age. The experimental animals were intravenously injected Escherichia coli LPS at a dose of 0.1 µg/kg b.w. Clinical observations and blood collection for laboratory examinations from the external neck vein were done just before endotoxin injection and then for 8 hours at one hour intervals and after 24, 48, 72 and 96 hr. from endotoxin application. All horses were examined routinely clinically (rectal temperature, pulse, respiration rate) and hematologically (total number of leukocytes, differential leukocyte count). Phagocytic activity of neutrophilic granulocytes was evaluated on the basis of the percentage of phagocytic cells (KF) and the phagocytic index (IF) using a standard Staphylococcus aureus strain. Moreover, the nitrotetrazolium blue reduction test (NBT) was done by a microquantity method. Clinical observations and hematological examinations in the experimental animals were typical for endotoxemia of horses. It was also found that LPS of E. coli at the administered dose stimulates unspecific immunity because it increases the value of KF, IF and NBT during the whole period of experimentation.
EN
The purpose of the study was to evaluate the metabolic activity of neutrophils in pure blood healthy and mastitic cows. Examinations were performed on 10 cows with clinical mastitis, 10 with subclinical mastitis and 10 healthy cows. The blood samples were collected from the coccygeal vessels and milk veins on the first day of clinical mastitis treatment with an intramammary product containing amoxicillin, clavulanic acid and prednisolone (or observation of the other cows) and on 3rd and 7th days. The blood was examined in the following ways: without in vitro stimulation and in vitro stimulation using fMLP, OZ, PMA. The reactive oxygen species (ROS) production was assessed by means of luminol-enhanced chemiluminescence (CL) using a kinetic method for 40 minutes at 38°C, measuring CL at 5 minute intervals (BioOrbit 1251 Luminometer). The area under the curve (integrate) was calculated. Significant differences in CL levels between peripheral blood and blood flowing from the udder were not found, irregardless of the use of stimulators or udder health status. The spontaneous and induced CL level, mainly through means of the receptor, was significantly higher in the blood of mastitic cows in comparison to healthy cows. PMNs from chronic mastitic cows were characterized by weakness of oxygen metabolism and a crucial increase of a stimulated respiratory burst through activation of the protein kinase C way (PMA). A significant decrease of OZ stimulated and non significant decrease of PMA stimulated CL was determined on the 3rd and 7th days after intramammary clinical mastitis treatment; however, the spontaneous CL remained on the same level.
PL
Celem pracy było zbadanie, czy istnieją zależności między liczbą granulocy- tów obojętnochłonnych znajdujących się w jamie ustnej a liczbą niektórych bytujących tu bakterii (paciorkowców, pałeczek hemofilnych, pałeczek kwasu mlekowego, pałeczek jelitowych, gronkowców i Streptococcus mutans). Ustalono, że poza pałeczkami jelitowymi, liczebność badanych grup bakterii statystycznie znamiennie ujemnie koreluje z liczbą neutrofili.
EN
The aim of the study was to evaluate the relationship between the number of polymorphonuclear leukocytes (PMNL) and the number of selected bacteria in human oral cavity. Sixty one healthy people (periodontal index of Russel's did not exceed 0.2), aged 22-26 were investigated. PMNL were isolated by rinsing oral cavity with isotonic, buffered NaCl solution. Nonstimulated whole saliva was bacteriologically examined. The number of PMNL, which was obtained from mouth of examined people was between 100000 and 4200000 in 100 mL of rinsings. Usually, i.e. in 34% of cases, the number was between 500000-1000000. Streptococci were isolated from all tested people and their number was the biggest. Almost in all cases there were isolated cariogenic bacteria - Streptococcus mutans (95%) and lactobacilli (93%). Haemophili, staphylococci, including Staphylococcus aureus, were found relatively often (in 86%, 86% and 52% respectively). Gram-negative enteric rods were rarely isolated (33%) and were the least numerous group of all. It has been stated, that there is a comparatively strong negative correlation between the number of PMNL in oral cavity and the number of streptococci (r=-0.55; p<0.0001), haemophili (r=-0.564; p<0.0001), staphylococci (r=-0.538; p<0.0001), lactobacilli (r=-0,407; p=0.0017) and Streptococcus mutans (r=-0.483; p=0.0002). The results suggest that PMNL are one of the factors controlling the number of some bacteria in human oral cavity.
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