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EN
The microclimate of a room has an impact on human well-being, physical and mental health, on work productivity and the preservation of good health. Several dozen species of bacteria can live in buildings and more than 400 species of fungi (mainly Aspergillus, Cladosporium, Penicillium, Fusarium genus). The presented results are studies from different health department suggesting the desirability of systematic microbiological testing, evaluation of fungal pathogens, and involving staff, patients, walls, floors, furniture units (hardware, underwear), and air. However the problem is a lack of unified Polish standards, the classes of microbiological indoor air and the lack of harmonization of existing rules for air sampling to assess air fungal pollution in the health care setting.
EN
ZnO-SnO2 nanocomposite was prepared by the sol-gel method. The as-prepared nanocomposite was characterized by X-ray diffraction (XRD), scanning electron micrograph (SEM-EDX), FTIR and UV-Visible spectrometer analysis techniques. The average particle size of nanocomposite was calculated from the XRD study. The average particle size of the prepared nanocomposite was 22 nm. According to the UV-Visible spectrum, the band gap value of 5.06 eV was obtained for the ZnO-SnO2nanocomposite. From the analysis techniques it was found that the metal oxides of ZnO-SnO2 mainly consist of ZnO and SnO2 metal oxides. The antibacterial and antifungal activities of the ZnO-SnO2nanocomposite were studied against Staphylococcus aureus (ATCC25923), Listeria monocytogenes (ATCC 11994)(Gram-positive), Salmonella typhi (ATCC14028), Escherichia coli (ATCC 25922)Gram-negative), Candida albicans (ATCC10231), and Aspergillus niger (ATCC 16404) (fungi)by two methods through the turbidity method or reading optical density and inhibition zone, which were carried out in the absence of irradiation. We observed an effective antibacterial and antifungal activity of the ZnO-SnO2 nanocomposite against bacteria and fungi.
EN
People use fresh, dried or marinated ginger in cooking, and some take it for their possible health benefits. Ginger may have anti-inflammatory, antibacterial, antiviral, and other healthful properties. Marinated ginger, mostly used for sushi on Polish market, is available in two variant forms: white and pink. Ginger is cut into thin slices, marinated in sweet and vinegar marinade and then undergoes thermal processing. The aim of the study was to assess the microbiological stability of marinated ginger. The best microbiological quality was characterized by ginger that had undergone a sterilization process. Although the initial number of lactic acid bacteria and fungi was the highest in ginger type C, the highest microbiological stability was also found in it.
EN
The work was aimed at studying abilities of soil microorganisms to participate in metal/radionuclide mobility processes by accumulating them. Soil microorganisms were treated with a mixture of heavy metals (Cr(III), Ni, Fe(III), Mn(II), Cd) in order to isolate the most tolerant ones. Among more metal-tolerant microorganisms microscopic fungi dominated. Tests of fungal tolerance towards each metal showed that the most tolerant fungi to almost all metals were Aspergillus niger, Penicillium oxalicum and Paecilomyces lilacinus. Accumulation ability of metal-tolerant fungi was tested using Pu(IV) and Fe(III). Investigation of Pu accumulation by fungal biomass showed that all the fungi accumulated Pu, and among the most effective radionuclide accumulators Eupenicillium sp., Penicillium oxalicum and Aspergillus niger could be mentioned. All the fungi showed high Fe-accumulation capacity. While growing in the medium with 1 mM iron, most fungi accumulated over 90% of Fe in their biomass. Very good accumulation and growth abilities in Fe-supplemented medium were demonstrated by Paecilomyces lilacinus.
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EN
The objective of the study was to characterize the mycological quality of air at animal veterinary practice in Krakow. Bioaerosol measurements were performed during the summer season of 2017. The samples of outdoor and indoor air at animal veterinary practice were collected using a 6-stage Andersen's air sampler. The highest concentration of fungal aerosol was observed in the treatment room. The analysis showed various fungal contamination in different measuring points at different measuring times of the day. Based on the analysis of bioaerosol particle size distribution it was found that the largest "load" of fungi, isolated form the air, can reach (in the human respiratory tract) to the region of the throat, trachei and primary bronchi. The predominant fungi in indoor air was Penicillium spp. and Cladosporium cladosporoides. Fungi that can cause dermatophytoses have also been isolated from indoor air: Microsporum canis and Trichophyton verrucosum. The study confirmed that the animal veterinary practice can be a workplace related to exposure to microbial agents.
EN
Playgrounds and sandpits (small architecture objects according to the Construction Law) are subject to meticulous supervision, both at the design stage and subsequent status checks of the objects. One of the requirements arising from the need to protect playgrounds from animals is the necessity for fencing the object (Regulation of 31 December 2002 On Safety and Hygiene in Public and Private Schools and Institutions; Polish Standard PN-EN 1176 Playground equipment and surfacing). Does fencing playgrounds really reduce contamination of sand? To verify this hypothesis, the studies have been conducted on the residential areas’ sandpits, both fence secured and unsecured, located in close proximity to one another. The aim of the study was to evaluate the effectiveness of fences and nets as protection from microbial and parasite contamination of sandpits, mainly due to the access of animals to them. For several seasons of spring and sum-mer the sand was examined in terms of the total number of heterotrophic bacteria and fungi (organic matter contamination of sand indicators) and for the presence of coliform bacteria (including Escherichia coli), bacteria of the Salmonella genus and the eggs of intestinal parasites. It can be concluded that fencing playgrounds affects sand pollution less with waste and plant material (as a consequence, it has been reported statistically significantly less heterotrophic bacteria and fungi in the fenced sandpits’ sand). Unfortunately, the fence does not eliminate the risks associated with sand pollution of coliform bacteria. Cats and birds, but also dogs, still have a continuous access to sand. Due to the repeatedly stated carelessness of children and their caregivers, gates left open to the playground do not constitute an obstacle for domestic and stray animals. Another source of sand pollution with intestinal pathogens can be a manner of carriage of new sand, as there is no legislation governing the issue of transport. It has been visually found that the sand has been transported in a vehicle used for waste disposal.
EN
The active substance of new herbicide generation is N-(2-pirydyloamino) methylenobisphosphonic acid. Two strains Fusarium culmorum CB3 and Fusarium oxysporum XVI, isolated from soil treated with examined substance, were chosen to investigation of using it as a sole source of phosphorus, nitrogen or carbon in the concentrations at 1.0 mM. Interactions between the dominating strains of fungi and N-(2-pirydyloamino) methylenobisphosphonic acid, mycelium growth rate, the spore germinate index, the level chemical changes of N-(2-pirydyloamino) methylenobisphosphonic acid and pH were investigated after cultivation in mineral medium and its modifications. The results of growth kinetics of the studied Fusarium fungi in the presence of N-(2-pirydyloamino) methylenobisphosphonic acid, were expressed by dry weight [g·dm-3]. Only Fusarium oxysporum XVI has been capable to grow in acidic medium (pH 4.0) using N-(2-pirydylamino) methylene-bisphosphonic acid as an alternative source of phosphorus. In these conditions the N-(2-pirydylamino) methylenebisphosphonic acid degradation was carried out to utilize phosphorus compounds as a source of nutrient components for this strain. The presence of N-(2-pirydylamino) methylenebisphosphonic acid receded a growth rate of mycelium but did not have an effect on spores of the tested fungi.
EN
Protective role of mycorrhizal fungus for plant against contamination with heavy metals makes a possibility to use fungi inocula for recultivation areas devastated by industry. Use of mycorrhizal symbiont for improving the living conditions of trees requires unambiguous identification of fungi that are able to form successful mycorrhizae in contaminated areas. We took up studies on identification of fungal species, which create mycorrhizal symbiosis with roots of birch growing on metallurgic heap. The identification of fungi collected directly from roots was performed by comparing patterns of restriction fragments of the fungal ITS products with DNA pattern obtained from fruiting bodies of Basidiomycetes fungi growing in the study area.
EN
The purpose of the study, carried out in the years 2001–2003 was to determine which fungal species inhabited decaying grapevine cuttings during callusing and soon after planting them into pots. The plant material was collected from 5 commercial plantations and 8 cultivars, which were most frequently cultivated. From each plantation and cultivar 20 cuttings with symptoms of the growth inhibition or decay were randomly sampled during the callusing period i.e. March/April (term I) and 2–3 months after planting the cuttings into pots i.e. June/July (term II). The results showed that from affected grapevine cuttings Phomopsis viticola, Botrytis cinerea, Alternaria alternata and Fusarium spp. were isolated most frequently. Moreover, it was found that after planting young cuttings into the pots, numerous isolates of soil borne pathogenes were obtained, among others Cylindrocarpon spp., Phytophthora sp., Rhizoctonia solani, Fusarium spp.
PL
Celem badań prowadzonych w latach 2001–2003 było określenie gatunków grzybów zasiedlających zamierające sadzonki winorośli, ze szczególnym uwzględnieniem gatunków fylosferowych. Do badań wytypowano 5 szkółek produkcyjnych, w różnych regionach Polski i 8 najczęściej uprawianych odmian. Z każdej plantacji i odmiany analizowano po 20 sadzonek z objawami zahamowania wzrostu lub zamierania. Próby pobierano w czasie kalusowania, tj. marzec/kwiecień (termin I) oraz po 2–3 miesiącach od wysadzenia roślin, tj. czerwiec/lipiec (termin II). Wykazano, że pędy zamierających sadzonek winorośli zasiedlały najczęściej Phomopsis viticola, Botrytis cinerea, Alternaria alternata i Fusarium spp. Ustalono, że łozy mateczne są głównym źródłem infekcji młodych roślin. Z korzeni badanych sadzonek uzyskiwano liczne gatunki przeżywające w glebie, m.in. Cylindrocarpon spp., Phythophthora sp., Rhizoctonia solani, Fusarium oxysporum i F. solani.
EN
Purpose: The aim of this study was the analysis of fungal air pollution in different rooms using different the air samplers. Material and methods: Air for mycological studies was collected from various hospital rooms. To monitor fungal air pollution were used samplers: SAS SUPER 100 (pbi-international), MAS 100 (Merck) and AIR IDEAL (BioMerieux). Results: We found differences in CFU/L values in depending on hospital room and the air sampler type. The CFU/L values of air samples taken by the AIR IDEAL ranged from 350 to 850 and (724.2 ± 159.9), and the CFU/L values of air samples taken by the sampler SAS SUPER 100 ranged from 160 to 800 (455.3 ± 250.73). The CFU/L values of air samples taken by the MAS 100 sampler varied from 50 to 1340 (302.5±56.6) From the air samples of both samplers was incubated 6 genera/species of fungi. Candia albicans fungi species and genus Penicillium species were most frequently isolated from SAS Super 100 sampler, and Penicillium species from the AIR IDEAL sampler. From the air samples of MAS 100 was isolated 11 types/species of fungi and in air samples of SAS Super 100 was isolated 7 types/species. Conclusions: Significant differences of CFU/L values in the tested rooms were found in depending on the used sampler. Mycological analysis of the obtained cultures from air samples suggests that there is not same isolation of fungi using the different samplers.
EN
We investigated the extraction of chitin with various solvents and changes in the amount of chitin and its molecular mass at different stages of maturity in the fruiting bodies of the fungi Laetiporus sulphureus, Tyromyces chioneus, Oudemansiella mucida, Lycoperdon perlatum, and Fomitopsis betulina. We extracted chitin from crushed mushroom fruiting bodies with 25% hydrochloric acid at 0°C. We characterised the molecular weight of reprecipitated purified chitin with a viscosimeter and Fourier-transform infrared spectroscopy. The mass of extracted chitin in all studied mushrooms initially increased from youth to maturity, and then decreased after ripening. At the same time, the molecular weight of chitin tended to increase.
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Content available Mushroom flavour
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PL
Grzyby w naszym otoczeniu stanowią nie tylko ciekawy świat kształtów, ale także są nośnikami ciekawych zapachów. Mykolodzy procujący z grzybami mikroskopowymi doskonale wiedzą, że niektóre z nich wydają charakterystyczną woń, jak np. woń czosnku typową dla rodzaju Mortierella lub kokosową w przypadku Trichoderma spp. W świecie grzybów spotkać można także takie zapachy, którym towarzyszy specyficzny smak. Któż nie zna typowego określenia „smak/aromat grzybowy”? Każdy, nawet osoba niezwiązana zawodowo z grzybami wie, co kryje się pod tym określeniem. Ale co stanowi, że możemy zdefiniować to określenie od strony chemicznej? Kluczową grupą substancji odpowiedzialnych za typowy smak i aromat grzybowy są lotne pochodne związków z grupy C8, a wśród nich najbardziej istotne okazują się być oct-1-en-3-ol, octan-3-ol, octan-3-on, oct-1-en-3-on. Grzyby dostarczają rozmaitych wrażeń zapachowych, począwszy od miłych anyżkowych,  owocowych, poprzez ogórkowe, czosnkowe, serowo-czosnkowe, następnie przypominające ziemniaki lub mąkę, aż do zapachów nieprzyjemnych, jak zapach karbidu lub fekaliów. Smak niektórych z nich jest skorelowany z zapachem, w wielu przypadkach smaków nie znamy. Za każdym z tych aromatów ukrywają się związki chemiczne pozwalające czasem na uzyskanie wstępnej informacji taksonomicznej. Bogactwo aromatów w świecie grzybów pozwala na biotechnologiczne wykorzystanie ich do otrzymywania bądź to czystych związków, jak np. w przypadku ketonu malinowego pozyskiwanego z Nidula niveotomentosa, bądź np. aromatycznej grzybni mogącej zastąpić rzadkie i pożądane gatunki grzybów leśnych, np. borowików.
EN
Mushrooms and fungi not only present a fascinating world of shapes, both macro- and microscopic, but they are also an interesting source of flavours, fragrances and odours, e.g. garlic, coconut, flour-like, cucumber or fruit-like, as well as the most characteristic for this kingdom of living organisms mushroom-like flavour and aroma. Fungi can possess many different and interesting flavours and fragrances – starting from nice anise-like, fruit-like, cucumber, garlic, to cheese-garlic, and ending with potato or flour-like smells. Some mushrooms emit carbide or distinctly faecal-like odour. The taste of mushrooms is frequently correlated with their aroma. What components does the core of a mushroom flavour consist of? Chemical analysis of specimens reveals compounds responsible for characteristic flavour and odour. It was found that the most characteristic flavour compound is defined mainly by C8 volatiles. Between all C8 compounds the most important for mushroom flavour are oct-1-en-3-ol, octan-3-ol, octan-3-on and oct-1-en-3-on. Fungi and mushrooms can enable biotechnological production of some flavour components, for instance the Nidula niveotomentosa produces a characteristic raspberries compound – raspberry ketone in submerged cultures; the biotechnological production can also provide rare and tasty forest mushroom biomass e.g. edible boletus.
EN
Isolation of some biologically important proteins from natural sources was found to be too expensive or scarcely possible (human proteins). The problem could be solved by expression of heterologous genes. Many biologically active proteins have been successfully expressed in filamentous fungi, some of them, however, at a low level. Thus, improvement of this technique appears to be a very important task. The process comprises several steps. Some of them, such as efficient transformation, vector construction, processing of signal sequences, post-translational modifications and secretion of the expressed proteins, have been intensively investigated. This review presents obstacles and problems encountered in expression of heterologous genes and discusses strategies of development in this area.
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