Wyniki wyszukiwania - Biblioteka Nauki

1

Inhibitory effect of resveratrol on free radical generation in blood platelets

100%

Olas B. , Żbikowska H. M. , Wachowicz B. , Krajewski T. , Buczyński A. , Magnuszewska A.

Acta Biochimica Polonica

|

1999

|

tom 46

|

nr 4

961-966

EN

Resveratrol (3,4',5-trihydroxystilbene), a compound found in many plants, has been shown to prevent coronary heart diseases and to exert a variety of antiinflammatory and anticancerogenic effects. It is effective in lowering the level of serum lipids and in inhibiting platelet aggregation. We evaluated the effect of trans-resveratrol on the production of free radicals in pig blood platelets and showed that resveratrol inhibited the production of different reactive oxygen species (O^·_2H^2O^2, singlet oxygen and organic radicals) measured by the luminol-dependent chemiluminescence in resting platelets (P < 0.05). Resveratrol inhibited also the generation of radicals in platelets activated by thrombin (P < 0.05). Treatment of platelets with resveratrol at concentrations of 6.25 and 12.5 μg/ml caused a statistically insignificant increase in the production of O^·-_2 in these cells, as measured by reduction of cytochrome c; however, at higher doses (25, 50 and 100 μg/ml) resveratrol distinctly reduced the generation of O^·-_2 in platelets (P < 0.05). We suggest that free radicals play an important role in the reduced reactivity of blood platelets induced by resveratrol.

2

Interactions of dietary carotenoids with singlet oxygen (1O2) and free radicals: potential effects for human health

100%

Böhm F. , Edge R. , George Truscott T.

Acta Biochimica Polonica

|

2012

|

tom 59

|

nr 1

27-30

EN

The dietary carotenoids provide photoprotection to photosynthetic organisms, the eye and the skin. The protection mechanisms involve both quenching of singlet oxygen and of damaging free radicals. The mechanisms for singlet oxygen quenching and protection against free radicals are quite different - indeed, under some conditions, quenching of free radicals can lead to a switch from a beneficial anti-oxidant process to damaging pro-oxidative situation. Furthermore, while skin protection involves β-carotene or lycopene from a tomato-rich diet, protection of the macula involves the hydroxyl-carotenoids (xanthophylls) zeaxanthin and lutein. Time resolved studies of singlet oxygen and free radicals and their interaction with carotenoids via pulsed laser and fast electron spectroscopy (pulse radiolysis) and the possible involvement of amino acids are discussed and used to (1) speculate on the anti- and pro-oxidative mechanisms, (2) determine the most efficient singlet oxygen quencher and (3) demonstrate the benefits to photoprotection of the eye from the xanthophylls rather than from hydrocarbon carotenoids such as β-carotene.

3

EPR examination of free radicals thermally formed invaselinum flavum

100%

Ramos P. , Pilawa B.

Nukleonika

|

2015

|

tom 60

|

nr 3

443-447

EN

The popular pharmaceutical base used in pharmacy – vaselinum flavum – was studied by an X-band (9.3 GHz) EPR spectrometer in the range of microwave power of 2.2–70 mW. The samples were sterilized in hot air oven at temperatures: 160°C (120 min), 170°C (60 min), and 180°C (30 min). The aim of this work was to determine properties and free radical concentrations in vaselinum flavum thermally sterilized at different conditions. The changes in free radical system in vaselinum flavum during storage were analyzed. Free radicals were found in all the heated samples. The lowest free radical concentration was obtained for vaselinum flavum heated at 180°C for 30 min; so these parameters are proposed for the thermal sterilization of this pharmaceutical base. Interactions with oxygen decreased free radical concentration in vaselinum flavum during storage. Strong quenching of free radicals in vaselinum flavum was observed after 2 days for the samples sterilized at temperatures 160 and 180°C. Such an effect for vaselinum flavum heated at temperature 170°C was observed later, 13 days after sterilization. Fast spin-lattice relaxation processes exist in thermally sterilized vaselinum flavum. The EPR lines of heated vaselinum flavum were homogeneously broadened. EPR spectroscopy and its use for examining the thermal sterilization process in pharmacy was confirmed.

4

Can angiotensin-converting enzyme inhibitors interfere with the free radicals? Measurement of antioxidant capacity using DPPH radical reduction examined by UV-VIS method

100%

Ramos P. , Juszczak A. , Szczołko W. , Pilawa B. , Stanisz B. J.

Acta Poloniae Pharmaceutica - Drug Research

|

2019

|

tom 76

|

nr 2

233-239

EN

A negative impact of radicals on human’s health is responsible for growing research interest in antioxidant properties of substances, which protect organisms from the damaging influence of these reactive species. Angiotensin-converting enzyme inhibitors (ACE-I) are the most popular drugs used in cardiovascular diseases. There are a lot of clinical reports that ACE-I have antioxidant properties, due to the fact, that prolonged use improves conditions of patients with neurodegenerative disorders and slow inflammatory processes. The paper shows the antioxidant properties of a selected ACE-I: cilazapril, ramipril, imidapril, lisinopril, perindopril, and quinapril. Among numerous methods for antioxidant activity estimation, DPPH reduction is the most popular and commonly used one due to its ease, speed, sensitivity and the usage of stable radicals. UV-Vis spectrophotometry was used to examine interactions of chosen ACE-I with model-free radicals. Absorption of UV-Vis spectra of DPPH (reference), and DPPH interacting with the tested ACE-I were compared. For all tested ACE-I kinetics of their interaction with DPPH, up to 30 minutes, were obtained. The strongest interaction with DPPH was observed for imidapril and cilazapril and the lowest interaction for lisinopril. Studies have shown usefulness UV-Vis spectrophotometry for obtaining information on interactions of ACE-I with model-free radicals.

5

EPR examination of free radicals thermally formed in vaselinum flavum

100%

Ramos P. , Pilawa B.

Nukleonika

|

2015

|

tom Vol. 60, No. 3, part 1

443--447

EN

The popular pharmaceutical base used in pharmacy – vaselinum flavum – was studied by an X-band (9.3 GHz) EPR spectrometer in the range of microwave power of 2.2–70 mW. The samples were sterilized in hot air oven at temperatures: 160°C (120 min), 170°C (60 min), and 180°C (30 min). The aim of this work was to determine properties and free radical concentrations in vaselinum flavum thermally sterilized at different conditions. The changes in free radical system in vaselinum flavum during storage were analyzed. Free radicals were found in all the heated samples. The lowest free radical concentration was obtained for vaselinum flavum heated at 180°C for 30 min; so these parameters are proposed for the thermal sterilization of this pharmaceutical base. Interactions with oxygen decreased free radical concentration in vaselinum flavum during storage. Strong quenching of free radicals in vaselinum flavum was observed after 2 days for the samples sterilized at temperatures 160 and 180°C. Such an effect for vaselinum flavum heated at temperature 170°C was observed later, 13 days after sterilization. Fast spin-lattice relaxation processes exist in thermally sterilized vaselinum flavum. The EPR lines of heated vaselinum flavum were homogeneously broadened. EPR spectroscopy and its use for examining the thermal sterilization process in pharmacy was confirmed.

6

Interaction between magnetic agglomerates and an extended free radicals network studied by magnetic resonance

100%

Guskos N. , Zolnierkiewicz G. , Typek J. , Guskos A. , Berczynski P. , Petridis D.

Open Physics

|

2012

|

tom 10

|

nr 1

166-171

EN

Solids containing an extended network of free radicals have been prepared and studied by magnetic resonance techniques in the 4–290 K temperature range. One solid contained additionally a small amount of magnetic γ-Fe2O3 in the form of nanoparticle agglomerates. The solid without agglomerates displayed only a narrow, single resonance line centered at g eff = 2.0043. The magnetic resonance measurements of the solid with γ-Fe2O3 agglomerates gave a spectrum composed of two lines attributed to two different magnetic centers: a narrow line due to free radicals and a broad line arising from magnetic iron oxide agglomerates. In the high temperature range the integrated intensities of both lines decreased with decreasing temperature. The resonance field of the broad line shifted to lower magnetic fields upon lowering the temperature with the gradient ΔH r/ΔT = 2.3 G/K, while the narrow line shifted towards higher magnetic fields. The linewidth of the broader line increased with decreasing temperature while for the narrow lines in both samples this change was small. The magnetic iron oxide clusters produce a magnetic field which acts on the free radicals network and its strength depends essentially on the concentration of clusters. The reorientation process in the free radicals network is more intense in the sample without magnetic clusters.

7

Influence of Vitamin E Given Intraperitoneally to Prevent Peritoneal Adhesions in Rats

100%

Makarska J. , Sosada K. , Stępien T. , Bucki B. , Żurawiński W.

Polish Journal of Surgery

|

2010

|

tom 82

|

nr 10

547-552

EN

The aim of the study was to evaluate the influence of vitamin E administered intraperitoneally on the prevention of peritoneal adhesion formation in rats on the basis of macroscopic and microscopic assessment of the adhesions.Material and methods. Experimental studies were performed on 50 Sprague-Dawley male rats, which were randomly divided into 5 groups, 10 rats in a group. Experimental group I (EI) included 10 rats which had peritoneal adhesions provoked by scraping of the wall of cecum and parietal peritoneum followed by intraperitoneal administration of vitamin E in the dose of 100 mg/kg body weight. Experimental group II (EII) included 10 rats which had peritoneal adhesions provoked by surgery, without administration of vitamin E. Control Group I (CI) included 10 rats which had the abdominal cavity opened without provoking peritoneal adhesions, and vitamin E was administered. Control Group II (CII) included 10 rats which had peritoneal adhesions provoked by surgery, and then lipid based solution was administered intraperitoneally. Control Group III (CIII) included 10 rats which had the abdominal cavity only opened and closed.Groups EI, CI and CII were the subject of the drugs intraperitoneal re-injection in first, second and third day after surgery. The animals were killed during the 8th postoperative day. Macroscopic examination of peritoneal adhesions using the classification reported by Nair was performed and samples for microscopic examination were excised.Results. In group EI peritoneal adhesions were formed in 60% rats (40% weak and 20% solid). In group EII peritoneal adhesions were found in all animals (30% weak and 70% solid). Reduction of the inflammatory response and less severe fibrosis were observed in animals with intraperitoneal administration of vitamin E.Conclusion. In the study, vitamin E administered intraperitoneally to rats decreased the intensity and extensiveness of peritoneal adhesions, which was confirmed by macroscopic and microscopic examinations.

8

Light Effect in Semiconductor Bridge Plasma Ignition

100%

Zhang L. , Li N. , Wan Z. , Zhu S.

Central European Journal of Energetic Materials

|

2017

|

tom Vol. 14, no. 4

996--1006

EN

Heat is considered to play an important role in Semiconductor Bridge (SCB) plasma ignition. Nevertheless, in this paper a non-heat effect is reported for SCB ignition of primary explosives. An initial comparison showed that there is no reasonable correlation between the ease of plasma ignition and the 5-s explosion temperature. Meanwhile the addition of Pb3O4 was found to make lead styphnate (LS) more active to SCB plasma ignition whereas the heat decomposition of this mixture was not accelerated. In terms of the phenomena mentioned above and the response of primary explosives to SCB plasma, we propose an effect of light in SCB plasma ignition. The free radical concentration change indicates that light enhances the activity of primary explosives in SCB plasma ignition. Regarding the mixture of LS and Pb3O4, the additive itself does not make LS sensitive to the SCB plasma. However, the supplement makes LS active under light exposure. As a result, the effect of light on SCB plasma ignition was confirmed by the experiments conducted in this study. This paper provides a new understanding of SCB plasma ignition from the viewpoint of explosives, which is of importance for the design of SCBs.

9

EPR studies of free radicals in thermally sterilized famotidine

100%

Ramos P. , Pilawa B. , Stroka E.

Nukleonika

|

2013

|

tom Vol. 58, No. 3

413--418

EN

Free radicals formation in thermally sterilized famotidine was studied by the use of an X-band (9.3 GHz) electron paramagnetic resonance (EPR) spectroscopy. The influence of temperature and time of sterilization on free radicals generated in famotidine was determined. The best sterilization conditions for the tested drug were found. Sterilization was done according to the pharmaceutical norms at temperatures 160°C (120 min), 170°C (60 min), and 180°C (30 min). It was pointed out that the optimal temperature of thermal sterilization for famotidine was 170°C and time of heating 60 min in this conditions the lowest free radicals concentration was found. The highest free radicals concentration was measured in famotidine heated at 160°C during 120 min, the long time of sterilization is responsible for this effect. Free radicals concentration changes during storage of famotidine after thermal sterilization. Its value increased with storage time for the drug sterilized at both temperatures of 170°C and 180°C. Free radicals concentration decreases from 7 days after sterilization. Complex system of free radicals with the shape of EPR lines, dependent on microwave power exist in thermally sterilized famotidine. Slow spin-lattice relaxation processes, strong dipolar interactions characterize thermally sterilized famotidine. Microwave saturation indicates that EPR lines of famotidine are homogeneously broadened. The usefulness of EPR spectroscopy to examine of the sterilized drugs was evaluated.

10

Effect of microwave power on EPR spectra of DOPA-melanin-netilmicin complexes with different drug concentrations : a study at temperatures in the range of 125-275 K

100%

Zdybel M. , Pilawa B. , Buszman E. , Wrześniok D. , Krzyminiewski R. , Kruczyński Z.

Nukleonika

|

2013

|

tom Vol. 58, No. 3

407--411

EN

Electron paramagnetic resonance (EPR) spectra of the model eumelanin – DOPA-melanin complexes with netilmicin measured with a microwave power in the range 0.3–200 mW were examined. The influence of microwave power on amplitudes (A) and linewidths (ΔBpp) of the spectra was determined. The EPR spectra were recorded by a BRUKER spectrometer at temperatures 125, 175, 225 and 275 K. Continuous microwave saturation of EPR lines was used to compare the acceleration of spin-lattice relaxation processes in melanin samples. Changes of the parameters of the EPR spectra of DOPA-melanin-netilmicin complexes, with the microwave power for the samples differing in netilmicin concentrations, were observed. The effect of temperature on the changes of the EPR spectra of the analyzed melanin complexes with the microwave power was determined. The spin-lattice relaxation is faster at higher temperatures for all the tested melanin complexes.

11

Spin traps in the detection of free radicals in the blood of patients with ischemia

100%

Krzyminiewski R. , Kruczynski Z. , Stepien A. , Dobosz B.

Polish Journal of Medical Physics And Engineering

|

2009

|

tom 15

|

nr 1

47-54

EN

Electron Paramagnetic Resonance and a nitrosobenzene spin trap were used to investigate free radicals in the human blood after angioplasty treatment. The nitrosobenzene anion radical was determined using EPR measurements and quantum-mechanical calculations. Differences were observed in the concentration of free radicals before and after angioplasty treatment. These results were compared with myocardium damage parameters (CPK, MB and TnT).

12

Melatonin and its metabolites: new findings regarding their production and their radical scavenging actions

88%

Reiter R. J. , Tan D.-x. , Pilar Terron M. , Flores L. J. , Czarnocki Z.

Acta Biochimica Polonica

|

2007

|

tom 54

|

nr 1

1-9

EN

This review summarizes some of the recent findings concerning the long-held tenet that the enzyme, N-acetyltransferase, which is involved in the production of N-acetylserotonin, the immediate precursor of melatonin, may in fact not always control the quantity of melatonin generated. New evidence from several different laboratories indicates that hydroxyindole-O-methyltransferase, which O-methylates N-acetylserotonin to melatonin may be rate-limiting in some cases. Also, the review makes the point that melatonin's actions are uncommonly widespread in organs due to the fact that it works via membrane receptors, nuclear receptors/binding sites and receptor-independent mechanisms, i.e., the direct scavenging of free radicals. Finally, the review briefly summarizes the actions of melatonin and its metabolites in the detoxification of oxygen and nitrogen-based free radicals and related non-radical products. Via these multiple processes, melatonin is capable of influencing the metabolism of every cell in the organism.

13

Effect of UV irradiation on free radicals in synthetic melanin and melanin biopolymer fromSepia officinalis– EPR examination

88%

Zdybel M. , Pilawa B.

Nukleonika

|

2015

|

tom 60

|

nr 3

483-488

EN

Free radicals in synthetic melanin and melanin from Sepia officinalis were studied by electron paramagnetic resonance (EPR) spectroscopy. The effect of time of ultraviolet (UV) irradiation on free radicals in these melanins was tested. The samples were exposed to UV during 15, 30, and 60 minutes. EPR spectra were measured with microwaves from an X-band (9.3 GHz) in the range of microwave power of 2.2–70 mW. The performed EPR examinations indicate that high concentrations (~1021–1022 spin/g) of o-semiquinone free radicals with g factors of 2.0039–2.0045 exist in all the tested samples. For nonirradiated samples, free radical concentration was higher in natural melanin than in synthetic melanin. UV irradiation caused the increase of free radical concentrations in synthetic melanin samples and this effect depends on the time of irradiation. The largest free radical formation in the both melanins was obtained for 60 min of UV irradiation. Free radical concentrations after the UV irradiation of melanins during 30 min were lower than during irradiation by 15 min, and probably this effect was the result of recombination of the radiatively formed free radicals. EPR lines of the tested samples broadened with increasing microwave power, so these lines were homogeneously broadened. The two types of melanins differed in the time of spin-lattice relaxation processes. Slower spin-lattice relaxation processes exist in melanin from Sepia officinalis than in synthetic melanin. UV irradiation did not change the time of spin-lattice relaxation processes in the tested melanins. The performed studies confirmed the usefulness of EPR spectroscopy in cosmetology and medicine.

14

Effect of microwave power on EPR spectra of thermally sterilizedeucerinum anhydricum

88%

Ramos P. , Pepliński P. , Pilawa B.

Nukleonika

|

2015

|

tom 60

|

nr 3

439-442

EN

Free radicals formed during thermal sterilization of eucerinum anhydricum – the pharmaceutical base were examined by an X-band (9.3 GHz) spectrometer. Eucerinum anhydricum was sterilized at different physical conditions according to the Polish Pharmacopeia norms. The samples were heated at temperatures: 160°C (120 min), 170°C (60 min), and 180°C (30 min). The aim of this study is to compare free radical concentration and effect of microwave power on EPR spectra of eucerinum anhydricum base thermally sterilized at different temperatures and periods of time. The effect of time storage on the free radicals in the heated samples was tested. Free radical concentrations in the sample stored 15 min strongly decreased with the increasing of sterilization temperature, probably as the result of recombination. Storage caused strong decrease of free radical concentrations in the samples, probably as the result of interactions with oxygen. It was observed to be independent of sterilization conditions from 2 days of storage and longer. Because of the lowest free radical concentration, for eucerinum anhydricum thermal sterilization at 180°C for 30 min is recommended. The sterilized samples should be stored at inert atmosphere without oxygen molecules. Fast spin-lattice relaxation processes existed in sterilized eucerinum anhydricum. The character of changes of amplitudes and linewidths of EPR lines with increasing of microwave power was the same for different storage times. The parameters of thermal sterilization and storage time influenced free radical concentration in eucerinum anhydricum, but magnetic spin-lattice interactions were unchanged. The usefulness of EPR spectroscopy in optimization of thermal sterilization process of eucerinum anhydricum was confirmed.

15

Comparison of the antioxidant potential of some herbal teas produced from ecological and traditional crops

88%

Wira D. , Zielonka-Brzezicka J. , Muzykiewicz-Szymańska A. , Klimowicz A.

Acta Universitatis Lodziensis. Folia Biologica et Oecologica

|

2021

|

tom 17

97-103

EN

The growing public awareness of the dangers regarding chemicals used in traditional agriculture has led to consumers seeking valuable and contaminant-free products. Ecological agriculture has become synonymous with high health value and product safety. The aim of this study was to evaluate the antioxidant activity and the total polyphenolic content of infusions of herbal tea bags and loose teas from traditional crops, as well as infusions of loose teas from ecological crops. Raw material comprised dried flowers of Matricaria chamomilla and Tilia cordata, as well as dried leaves of Urtica dioica, Melissa officinalis and Mentha piperita. Herbal infusions were prepared using three brewing times: 5, 10 and 20 min. The analysis of antioxidant potential was performed using in vitro methods such as DPPH, ABTS and FRAP. The polyphenolic content was determined using the Folin-Ciocalteu method. The antioxidant activity of the studied tea infusion depended on the method by which the plants were cultivated and the brewing time. The ecological agriculture conditions seem not to stimulate the synthesis of antioxidants. However, the possibility to obtain other beneficial properties of the studied plants is an indication to carry out ecological cultivation.

16

Effect of UV irradiation on free radicals in synthetic melanin and melanin biopolymer from Sepia offi cinalis : EPR examination

88%

Zdybel M. , Pilawa B.

Nukleonika

|

2015

|

tom Vol. 60, No. 3, part 1

483--488

EN

Free radicals in synthetic melanin and melanin from Sepia officinalis were studied by electron paramagnetic resonance (EPR) spectroscopy. The effect of time of ultraviolet (UV) irradiation on free radicals in these melanins was tested. The samples were exposed to UV during 15, 30, and 60 minutes. EPR spectra were measured with microwaves from an X-band (9.3 GHz) in the range of microwave power of 2.2–70 mW. The performed EPR examinations indicate that high concentrations (~1021–1022 spin/g) of o-semiquinone free radicals with g factors of 2.0039–2.0045 exist in all the tested samples. For nonirradiated samples, free radical concentration was higher in natural melanin than in synthetic melanin. UV irradiation caused the increase of free radical concentrations in synthetic melanin samples and this effect depends on the time of irradiation. The largest free radical formation in the both melanins was obtained for 60 min of UV irradiation. Free radical concentrations after the UV irradiation of melanins during 30 min were lower than during irradiation by 15 min, and probably this effect was the result of recombination of the radiatively formed free radicals. EPR lines of the tested samples broadened with increasing microwave power, so these lines were homogeneously broadened. The two types of melanins differed in the time of spin-lattice relaxation processes. Slower spin-lattice relaxation processes exist in melanin from Sepia officinalis than in synthetic melanin. UV irradiation did not change the time of spin-lattice relaxation processes in the tested melanins. The performed studies confirmed the usefulness of EPR spectroscopy in cosmetology and medicine.

17

Preparation of N-aryl-ketonitrone spin traps

88%

Hassan I. , Lauricella R. , Tuccio B.

Open Chemistry

|

2006

|

tom 4

|

nr 2

338-350

EN

The syntheses of seven N-aryl-C,C-dialkoxycarbonylnitrones 1–7, six of which were original, were achieved from the appropriate aryl-nitroso compounds. These ketonitrones were found to trap efficiently carbon-centred free radicals in aqueous media, yielding stable aminoxyl radicals whose EPR spectra lasted several days. The two penta-deuterated compounds 6 and 7 were also found to be efficient at trapping methoxyl radical. Their various spin adducts showed simple three line signals, very sensitive to the polarity of the environment. This study represents the very first use of linear ketonitrones as spin traps.

18

EPR studies of Cladosporium cladosporioides complexes with amphotericin B

88%

Zdybel M. , Pilawa B. , Buszman E. , Witoszyńska T.

Nukleonika

|

2013

|

tom Vol. 58, No. 3

401--405

EN

Free radicals in Cladosporium cladosporioides cultured with amphotericin B were spectroscopically studied. The effect of drug concentrations on free radicals in the fungal melanin biopolymer was examined. It was pointed out that the concentrations of free radicals in the complexes of Cladosporium cladosporioides mycelium with amphotericin B depend on the drug concentrations. Eumelanin dominates in the fungal studied samples. The slow spin-lattice relaxation processes exist in the tested samples. Free radicals take a part in the formation of Cladosporium cladosporioides mycelium complexes with amphotericin B.

19

Effect of microwave power on EPR spectra of thermally sterilized eucerinum anhydricum

88%

Ramos P. , Pepliński P. , Pilawa B.

Nukleonika

|

2015

|

tom Vol. 60, No. 3, part 1

439--442

EN

Free radicals formed during thermal sterilization of eucerinum anhydricum – the pharmaceutical base were examined by an X-band (9.3 GHz) spectrometer. Eucerinum anhydricum was sterilized at different physical conditions according to the Polish Pharmacopeia norms. The samples were heated at temperatures: 160°C (120 min), 170°C (60 min), and 180°C (30 min). The aim of this study is to compare free radical concentration and effect of microwave power on EPR spectra of eucerinum anhydricum base thermally sterilized at different temperatures and periods of time. The effect of time storage on the free radicals in the heated samples was tested. Free radical concentrations in the sample stored 15 min strongly decreased with the increasing of sterilization temperature, probably as the result of recombination. Storage caused strong decrease of free radical concentrations in the samples, probably as the result of interactions with oxygen. It was observed to be independent of sterilization conditions from 2 days of storage and longer. Because of the lowest free radical concentration, for eucerinum anhydricum thermal sterilization at 180°C for 30 min is recommended. The sterilized samples should be stored at inert atmosphere without oxygen molecules. Fast spin-lattice relaxation processes existed in sterilized eucerinum anhydricum. The character of changes of amplitudes and linewidths of EPR lines with increasing of microwave power was the same for different storage times. The parameters of thermal sterilization and storage time influenced free radical concentration in eucerinum anhydricum, but magnetic spin-lattice interactions were unchanged. The usefulness of EPR spectroscopy in optimization of thermal sterilization process of eucerinum anhydricum was confirmed.

20

Human Tissue Investigations Using PALS Technique - Free Radicals Influence

88%

Jasińska B. , Zgardzińska B. , Chołubek G. , Pietrow M. , Gorgol M. , Wiktor K. , Wysogląd K. , Białas P. , Curceanu C. , Czerwiński E. , Dulski K. , Gajos A. , Głowacz B. , Hiesmayr B. , Jodłowska-Jędrych B. , Kamińska D. , Korcyl G. , Kowalski P. , Kozik T. , Krawczyk N. , Krzemień W. , Kubicz E. , Mohammed M. , Pawlik-Niedźwiecka M. , Niedźwiecki S. , Pałka M. , Raczyński L. , Rudy Z. , Sharma N. , Sharma S. , Shopa R. , Silarski M. , Skurzok M. , Wieczorek A. , Wiktor H. , Wiślicki W. , Zieliński M. , Moskal P.

Acta Physica Polonica A

|

2017

|

tom 132

|

nr 5

1556-1558

EN

The positron annihilation lifetime spectroscopy was applied to the samples of the human uterine leiomyomas and the normal myometrium tissues taken from the selected place of the uterus during a surgery. The method indicated differences in values of the measured positron annihilation lifetime spectroscopy parameters (lifetimes and intensities) between healthy and diseased tissue samples. The additional measurements were performed either in darkness or in presence of visible light which influenced the free radicals present in both kind of tissues and, as a result, made changes in free annihilation and o-Ps decay lifetime and intensity values.