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EN
In the framework of the performed studies, the antibacterial activity of the following fungicides was evaluated: Miedzian 50 WG (active substance - a.s. 50% copper oxychloride), Ridomil MZ Gold 68 WG (a.s. 3.8% metalaxyl-M and 64%, mancozeb), Euparen Multi 50 WG (a.s. 50% tolylfluanid), Captan 80 WG [a.s. 80% N-(captan)], Dithane Neotec 75 WG (a.s. 75% mancozeb). The evaluation also concerned the essential oils: lavender, sage, lemon balm, clove, and a preparation based on thyme oil (BioZell). Each preparation and compound was tested against the following bacterial pathogens: Erwinia amylovora, Xanthomonas arboricola pv. corylina, X. arboricola pv. juglandis, Pseudomonas syringae pv. syringae, Agrobacterium tumefaciens (presently Rhizobium radiobacter). Each preparation and compound was tested at a concentration of 1,000 ppm of active substance. Copper oxychloride was also tested at a concentration of 1,500 ppm. Among the tested fungicides, metalaxyl-M with mancozeb, mancozeb alone, and copper oxychloride inhibited all of the tested strains of pathogenic bacteria. Tolylfluanid did not inhibit any of the bacteria used. Out of the investigated essential oils, the strongest inhibitors of bacteria were: sage, cloves, and BioZell. The protective activity of the above mentioned fungicides was also evaluated in vivo. They were assessed against fire blight on apple blossoms and pear fruitlets, against bacterial canker on sweet cherry fruitlets, and against crown gall on sunflower seedlings (the test plant). All fungicides were applied at the same concentrations as those in the in vitro tests. Only copper oxychloride was found to show protective activity against the studied diseases. This result indicates that the antibacterial properties of the other fungicides did not correspond with their activity on the plant organs used in the in vivo experiment.
EN
Relative to the established and well known rice diseases, sheath brown rot caused by Pseudomonas fuscovaginae can be considered new but getting widespread and serious all over the world. Our research was aimed to monitor and quantify the incidence and importance of the disease in Malaysia. A series of field monitoring and sampling were conducted to quantify the incidence and severity of the disease. Laboratory analysis of the collected diseased plant was done to identify the causal organism. Disease resistance screening of selected rice cultivars were also conducted to overcome the problem. The disease was found to become more important, prevalent and widely spread throughout rice growing areas in Peninsular Malaysia. Infected plants in the field became yellowish, lower leaf sheaths turned light or dark brown, while grains produced by an infected plant were discoloured, malformed and empty. The highest disease incidence was recorded in the state of Pahang (62%) and Selangor (62%), while the most severe infection was recorded in Pahang (55%) and Terengganu (61%). The evaluations of varietal resistance evaluation showed that the pathogen naturally infected all tested rice varieties at different levels of infection. Several rice varieties i.e. MR240, MR243, MR244, MR245, MR246, MR248 and MR249, classified as moderately resistant to the disease, could be recommended for planting in the next planting season.
PL
Brązowa zgnilizna pochew liściowych ryżu wywoływana przez Pseudomonas fuscovaginae może być uznana w porównaniu do innych, powszechnie występujących i dobrze znanych chorób ryżu za nową, ale rozprzestrzeniającą się i ważną na całym świecie. Podjęte badania miały na celu monitorowanie i zbadanie jej występowania, i znaczenia na Malajach. W celu określenia występowania i nasilenia choroby wykonano seryjny monitoring oraz przeprowadzono pobieranie prób. Wykonano analizy labolatoryjne zebranych roślin w celu określenia sprawcy choroby. Przeprowadzono testowanie wybranych odmian ryżu pod kątem ich odporności na patogena celem ograniczenia jego występowania. Wykazano, że choroba nabiera znaczenia i często występuje we wszystkich rejonach uprawy ryżu na półwyspie Malajskim. W polu zakażone rośliny żółkną, dolna pochwa liściowa staje się jasno- i ciemnobrązowa, a ziarno wytworzone przez chorą roślinę jest przebarwione, zniekształcone i puste. Najczęstsze występowanie choroby zanotowano w stanie Pahang (62%) i Selangor (62%), podczas gdy najsilniejsze porażenie stwierdzono w Pahang (55%0 i Terengganu (61%). Ocena odporności odmian wykazała, że patogen porażał naturalnie wszystkie odmiany ryżu, w zróżnicowanym stopniu. Kilka odmian zaklasyfikowano jako średnio odporne, t.j.: MR240, MR243, MR244, MR245, MR246, MR248 i MR 249, mogło być polecane do sadzenia w następnym sezonie wegetacyjnym.
EN
The API 20E test and standard methods were applied to examine biochemical features of P. multocida strains. All tested isolates decomposed glucose, mannitol and saccharose. Sorbitol and arabinose were fermented by 82% and 43% of the strains, respectively. Xylose, trehalose, and dulcitol were fermented by 30%, 13% and 4% of isolates, respectively. All the tested strains produced catalase, ornithine decarboxylase, cytochrome-oxidase, indole, and reduced nitrates to nitrites. Moreover, the strains did not reveal motility and did not grow on MacConkey agar and failed to ferment maltose. Our findings enabled the recognition of ten distinct biochemical types, termed biovars I to X, and permitted the assignation of the strains into 3 subspecies. Of the isolates tested, 78% were identified as P. multocida subsp. multocida, 8.1 % as P. multocida subsp. septica, and 4% as P. multocida subsp. gallicida according to Mutters et al. ( 12). On the other hand, 9.7% of the strains were not classified as one of the three subspecies.
9
Content available Epidemiology of Salmonella and Salmonellosis
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EN
The prevalence of enteritis and its accompanying diarrheal and other health challenges linked to infections with Salmonella has continuously plagued sub Saharan Africa. In Nigeria, typhoid fever is among the major widespread diseases affecting both young and old as a result of many interrelated factors such as inadequate sanitaion, indiscriminate use of antibiotics and fecal contamination of water sources. Morbidity associated with illness due to Salmonella continues to increase with untold fatal consequences, often resulting in death. An accurate figure of cases is difficult to arrive at because only large outbreaks are mostly investigated whereas sporadic cases are under-reported. A vast majority of rural dwellers in Africa often resort to self medication or seek no treatment at all, hence serving as carries of this disease. Non typhoidal cases of salmonellosis account for about 1.3 billion cases with 3 million deaths annually. Given the magnitude of the economic losses incurred by African nations in the battle against salmonella and salmonellosis, this article takes a critical look at the genus Salmonella, its morphology, isolation, physiological and biochemical characteristics, typing methods, methods of detection, virulence factor, epidemiology and methods of spread within the environment.
EN
Specific bacterial disease symptoms were observed on onion bulbs in almost all regions in Poland. For the purpose of identification of agents causing disease, bacteria were isolated from the symptomatic plants. Their pathogenicity was confirmed by using pathogenicity test on onion scales. These bacteria were identified biochemically and molecularly as Serratia plymuthica.
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EN
Silicon plays an important role in providing tolerance to various abiotic stresses and augmenting plant resistance against diseases. However, there is a paucity of reports about the effect of silicon on bacterial and viral pathogens of plants. In general, the effect of silicon on plant resistance against bacterial diseases is considered to be due to either physical defense or increased biochemical defense. In this study, the interaction between silicon foliar or soil-treatments and reduced bacterial and viral severity was reviewed. The current review explains the agricultural importance of silicon in plants, refers to the control of bacterial pathogens in different crop plants by silicon application, and underlines the different mechanisms of silicon-enhanced resistance. A section about the effect of silicon in decreasing viral disease intensity was highlighted. By combining the data presented in this study, a better comprehension of the complex interaction between silicon foliar- or soil-applications and bacterial and viral plant diseases could be achieved.
EN
Bacterial leaf blight is one of the major diseases in rice and affects yields. Thus, various methods have been applied to protect rice from this disease. Here, we show systemic translocation of the human drug niclosamide (5-chloro-N-(2-chloro-4-nitrophenyl)- 2-hydroxybenzamide) in rice and its long-term effect on prevention of rice leaf blight. The development of Xanthomonas oryzae pv. oryzae-induced rice leaf blight was effectively inhibited in untreated systemic leaves as in niclosamide-treated leaves, although its effect gradually decreased in a time-dependent manner. Time-course examination after niclosamide treatment showed that the niclosamide level was highest after 3 h in non-treated distal leaves, suggesting fast systemic movement of niclosamide from the treated local site to untreated distal regions. Our data indicate that niclosamide controls rice leaf blight by its rapid systemic movement and that its effect is maintained for a long time.
EN
Tea infusions are consumed frequently and in large amounts, so they may play a role in modelling intestinal microflora or in preventing bacterial diseases. The present study examined the effect of different kinds of tea (black, green and puerh) on Lactobacillus rhamnosus, Lactobacillus plantarum, Leuconostoc mesenteroides subsp. mesenteroides, Escherichia coli, Staphylococcus aureus and Salmonella enteritidis. In order to ascertain the connection between antioxidant and antimicrobial properties, the content of polyphenolic compounds and the reducing power of the teas were assessed. The rate of bacterial proliferation was measured by calculating the generation time between the 2nd and 6th hour of incubation. Staphylococcus aureus was the most sensitive to the addition of tea extracts to the media. Among lactobacilli (LAB), Lactobacillus casei proved to be sensitive mainly to the extracts of black and puerh tea. The value for reducing power does not fully correspond to the content of polyphenols and antimicrobial properties.
PL
Napary herbaciane konsumowane regularnie i w dużych ilościach mogą odgrywać rolę w modelowaniu mikroflory jelit lub zapobieganiu chorobom bakteryjnym. W pracy badano wpływ różnych rodzajów herbat (czarnej, zielonej i puerh) na wzrost Lactobacillus rhamnosus, Lactobacillus plantarum, Leuconostoc mesenteroides subsp. mesenteroides, Escherichia coli, Staphylococcus aureus i Salmonella enteritidis. Zależność pomiędzy właściwościami antyoksydacyjnymi i antybakteryjnymi określano, oznaczając zawartość związków polifenolowych i siłę redukującą naparów, natomiast szybkość wzrostu bakterii mierzono, obliczając czas generacji pomiędzy 2 i 6 godziną inkubacji. Staphylococcu aureus był najbardziej wrażliwy na dodatek herbat do podłoży. Pośród bakterii kwasu mlekowego Lactobacillus casei wykazywał największą wrażliwość głównie w stosunku do ekstraktów herbaty czarnej i puerh. Wartość siły redukującej nie w pełni korespondowała z zawartością polifenoli i właściwościami antymikrobiologicznymi ekstraktów.
EN
This study analysed the pattern of antibiotic resistance in 251 Campylobacter strains isolated from symptomatic children hospitalized in 4 large paediatric hospitals in Poland from 2000 through 2007. The highest resistance was found for ciprofloxacin (49.5% for C. jejuni and 51.3% for C. coli), followed by tetracycline (17.5% and 18.0%, respectively), and ampicillin (13.2% and 10.2%, respectively). Almost all isolates were susceptible to macrolides. As much as 22.6% of C. jejuni and 25.6% of C. coli were resistant to more than one class of antimicrobial agents. Multidrug resistance (defined as resistance to at least two classes of antimicrobials) rose significantly from 5.1% in 2000-2003 to 34.6% in 2004-2007.
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