Wyniki wyszukiwania - Biblioteka Nauki

1

Antioxidant effect of natural honeys affected by their source and origin

100%

Mellen M. , Fikselova M. , Medelova A. , Hascik P.

Polish Journal of Food and Nutrition Sciences

|

2015

|

tom 65

|

nr 2

EN

In purpose to examine the antioxidant activity of 15 natural honeys of different origin ABTS method was used, total phenol content and dry matter content of honey samples were determined. Honeys were collected from different locations of Slovakia, Poland and Serbia and were represented as monofl oral and multifl oral samples (10) which originated from Poland and Slovakia, forest samples (4) originated from Serbia and honeydew honey. Average values of antioxidant activity observed in samples of honeys ranged from 0.62 to 4.63 mmol/kg. The highest antioxidant activity was detected in buckwheat honey and the lowest was shown in acacia honey. By observing the impact of individual honey samples on antioxidant activity it was found that the sample had a highly statistically signifi cant effect. 10 homogeneous groups which varied in the antioxidant activity among each other were established by all 15 samples. Antioxidant activity of honeys could be a positive infl uence factor in terms of honey differentiation, especially in the case of the forest honeys collected from different places. Monofl oral and multifl oral honeys (10) established 5 homogenous groups, but in the case of several multifl oral honeys which originated from different places of Poland and Slovakia no statistically signifi cant differences were found.

2

Antioxidative effect of plant extracts and flavones on liposome and erythrocyte membranes

100%

Gabrielska J. , Korzeniowska M. , Wojdylo A.

Polish Journal of Food and Nutrition Sciences

|

2007

|

tom 57

|

nr 4[A]

145-150

EN

The paper describes the use of liposomes and erythrocytes membrane as a real membrane models to evaluate the potential benefits of several plants extracts and two flavones in relation to lipid peroxidation. The antioxidant behaviour of the plant extracts from pine (Pinus silvestris L), hawthorn (Crataegus oxyacantha L, two extracts: from hawthorn’s leaves-l and bark-b), evening primrose (Oenothena paradoxa Hudziok – three extracts differ in procyanidins content P1, P2 and P3) and rosemary (Rosmarinus officinalis, as a standard for extracts) and flavones of baicalin and rutin have been studied. The results obtained showed that the studied extracts exhibited differentiated, dose-dependent antioxidant activity against phospatidylcholine liposomes (rosemary>pine≈hawthorn-l>hawthorn-b≈P1≈P2≈P3; statistically significant differences were observed between the extracts at p≤0.05) and erythrocyte membranes (rosemary≥hawthorn-b≈hawthorn-l>P1≈pine>P2≈P3) when the oxidation was induced by UV-C radiation. They also reduce the oxidation of liposomes and erythrocyte membrane when its oxidation was induced by 2,2’-azobis(2-amidinopropane) dihydrochloride (pine≥P1≈P2≈rosemary≈P3 in the case of liposomes and rosemary>> pine≥P1≈P2≥P3 in a case of erythrocyte). Moreover, the results of the study show that baicalin is characterised by high inhibition ability towards liposome PC peroxidation, as well as towards erythrocyte ghosts, when oxidation was initiated by UV radiation. However, at the same experimental conditions, the inhibitory capacity of rutin was about 7-8 times weaker. The presence of cholesterol in liposome membrane decreased the level of membrane peroxidation but do not influenced on the antioxidant activity of hawthorn extract.

PL

W pracy zastosowano liposomy oraz błony erytrocytów jako modele błon biologicznych do opisu procesu utleniania lipidów. Zbadano antyoksydacyjne działanie ekstraktów roślinnych z sosny (Pinus silvestris L), głogu (Crataegus oxyacantha L,dwa ekstrakty: z liści i kory głogu), wiesiołka (Oenothena paradoxa Hudziok, trzy ekstrakty różniące się zawartością procyjanidyn oznaczone P1, P2 and P3) and rozmarynu (Rosmarinus officinalis, jako standardu dla ekstraktów) oraz flawony bajkaliny i rutyny. Uzyskane wyniki badań wykazały zależną od stężenia, zróżnicowaną aktywność antyoksydacyjną ekstraktów w stosunku do błon liposomów (rozmaryn>sosna≈głóg-l>głóg-b≈P1≈P2≈P3; oraz błon erytrocytów (rozmaryn≥głóg-b≈głóg-l>P1≈sosna>P2≈P3) w sytuacji gdy utlenianie indukowano promieniowaniem UV-C. Redukują one także utlenienie liposomów i błon erytrocytów indukowane 2,2’-azobis(2-amidinopropane)dihydrochloride (AAPH) (sosna≥P1≈P2≈rozmaryn≈P3, w przypadku liposomów and rozmaryn>>sosna≥P1≈P2≥P3, w przypadku erytrocytów). Bajkalina posiada wysoką zdolność inhibicji peroksydacji zarówno liposomów PC jak i cieni erytrocytów w sytuacji utleniania indukowango promieniami UV, natomiast rutyna w tych warunkach jest ok. 7-8 krotnie słabszym inhibitorem procesu. Obecność cholesterolu w błonach liposomów w zasadzie zmniejsza poziom peroksydacji liposomów (różnice istotne statystycznie) lecz nie wpływa na aktywność antyoksydacyjną ekstraktu z liści głogu.

3

Effect of antioxidant supplemented-diet for blue fox on chosen reproductive and performance parameters

86%

Likos-Grzesiak B. , Martyna J. , Wnuk W. , Saba L. , Nowakowicz-Debek B. , Ondrasovic M.

Annales Universitatis Mariae Curie-Skłodowska. Sectio EE: Zootechnica

|

2008

|

tom 26

|

nr 3

1-8

4

Amifostine antioxidant effect on serum of rats treated with cyclophosphodamide

86%

Stankiewicz A. , Skrzydlewska E.

Polish Journal of Environmental Studies

|

2005

|

tom 14

|

nr 3

EN

Amifostine is one of the cytoprotective drugs used during anticancer therapy. Amifostine as a thiol compound possesses antioxidant properties and protects only healthy cells against damage, mainly by scavenging reactivity oxygen species, competing with oxygen to prevent oxygen radical interactions with DNA, and promoting cell repair through hydrogen donation to reactive oxygen species. The aim of the present study was to evaluate antioxidative ability of amifostine in blood serum of rats exposed to cyclophosphamide during two weeks after drug administration. We show that amifostine only to a small degree prevents disorganisation of antioxidant systems of blood serum of rats caused by cyclophosphamide action. It is probably connected with low concentrations of amifostine active metabolites in the serum.

5

Investigation into the anti-radical activity of extracts obtained from Uncaria tomentosa Willd DC

86%

Sroka Z. , Fecka I. , Cisowski W. , Luczkiewicz M.

Herba Polonica

|

2001

|

tom 47

|

nr 3

PL

Z kory korzeni oraz sproszkowanego preparatu (EA) pochodzących z Uncaria tomentosa uzyskano wyciągi bogate w związki fenolowe. Wykazano silną aktywność przeciwwolnorodnikową tych wyciągów. Najsilniejszą aktywność przeciwwolnorodnikową wykazano dla wyciągu WMP otrzymanego z EA (MeOH - woda, 1:1). Wyciąg ten w stężeniach 0.036 i 0.072 mg/ml spowodował zmniejszenie stężenia wolnego rodnika DPPH odpowiednio o 64.6% i 92.8%.

EN

The root bark and pulverised extract (EA) originating from Uncaria tomentosa were used to obtain extracts which contained the full set of of phenolic compounds. Tests of all of these extracts indicated powerful anti-radical activity. The strongest anti-radical effect was demonstrated for WMP extract (MeOH - water, 1:1) obtained from EA. Concentrations of 0.036 and 0.072 mg/ml of this extract in the tested solution yielded decreases in DPPH radical concentration of 64.6% and 92.8% respectively.

6

Antioxidative effects of phenolic compounds of mushroom mycelia in simulated regions of the human colon, in vitro study

72%

Vamanu E. , Pelinescu D. , Avram I.

Polish Journal of Food and Nutrition Sciences

|

2018

|

tom 68

|

nr 1

EN

Many compounds in mushrooms are biologically active; however, the in vivo actions of their metabolites are poorly understood. An in vitro system, GIS1, was used to simulate the fermentation action of microbiota in each colon region. We used MycoPo, a natural product obtained from the lyophilized mycelia of different Pleurotus ostreatus species to determine the biological effects in human-colon regions. Controls (Lentinula edodes mycelia; dried basidia of Agaricus brunnescens) were chosen to confi rm the biological activity of P. ostreatus mycelia in vitro. We measured total antioxidant capacity and ferric ion-reducing antioxidant power (FRAP) in simulated colon regions to identify antioxidant compounds, and undertook in vitro gastrointestinal simulation and microbiological analyses. The highest FRAP was found for the ascending colon, and the antioxidant effect was higher when MycoPo was administered. A. brunnescens consumption resulted in low total antioxidant capacity. Polyphenol content was correlated with the antioxidant status and microbial composition of microbiota. Total polyphenolic content was higher after A. brunnescens consumption, and four types of polyphenols were identifi ed by high-performance liquid chromatography. Major phenolic acids were gentisic acid, homogentisic acid, and small amounts of caffeic acid. The Enterobacteriaceae species populations varied greatly across the three parts of the colon. We noted a signifi cant (p<0.01) correlation between antioxidant status in the transverse and descending colon after MycoPo administration, and A. brunnescens consumption with the number of Lactobacillus and Bifi dobacteria species (R2 >0.85). These data suggest a direct relationship between favorable bacterial strains and availability of bioactive compounds, with specifi city for each colon region.

7

Possible role of garlic oil in ameliorating renal injury after liver ischemia/reperfusion in rats

72%

Lasheen N. N. , Elayat W. M. , Elrefai M. F. M. , Zaki W. S. , Ahmed E. H. , El Sheikh R. M. N. , Abo Raya D. S. A. , Gad F. R. S.

Journal of Physiology and Pharmacology

|

2019

|

tom 70

|

nr 5

8

The influence of Viburnum opulus polyphenolic compounds on metabolic activity and migration of HeLa and MCF cells

72%

Zakłos-Szyda M. , Pawlik N.

Acta Innovations

|

2019

|

tom no. 31

33--42

EN

In recent years, research of antitumor activity of natural compounds isolated from plant material has increased. Polyphenols have gained significant attention due to their proapoptotic abilities and their involvement in migration and inhibition of metastasis processes. The anticancer effects of polyphenolic extracts of Viburnum opulus fruit against human breast (MCF-7) and cervical (HeLa) cancer cell lines have been confirmed in this study. It was demonstrated that the tested preparations (methanol – M and acetone – A from pomace, juice – J and juice after extraction to the solid phase SPE – PF) show cytotoxic activity and regulate the migration process of cancer cells. The degree of inhibition of cell migration was measured at two times - 24 h and 48 h after addition of the tested preparations. The highest toxicity towards both cell lines was demonstrated by the polyphenol fraction obtained after juice purification SPE (IC50 values at concentration of 63,541 and 19,380 μg/mL for HeLa and MCF cell lines, respectively). At the same time, the same preparation inhibited cell migration the most (nearly 70% compared to controls at both times at the concertation of 15 and 30 μg/mL). All preparations showed the antioxidant ability, but the Viburnum opulus juice (200 and 350 μg/mL) and the preparation after its purification (15 and 30 μg/mL) have larger ability to inhibit the intracellular oxidative stress (30-40%) than preparation obtained from pomace (nearly by 20% at concentration of 20 and 50 μg/mL of M and A). Despite the antioxidative capacity of the preparations, they simultaneously decreased cellular mitochondrial potential. The results obtained indicate the high potential of components of Viburnum opulus polyphenolic compounds can be used in the production of innovative dietary supplements or pharmacological preparations for people with an increased risk or inclination towards developing breast or cervical cancer.

9

Further studies on the anti-oxidative effect of putrescine in sodium nitrite-treated rats

72%

Grudzinski I. P. , Frankiewicz-Jozko A.

Roczniki Państwowego Zakładu Higieny

|

2002

|

tom 53

|

nr 1

EN

Studies were carried out to elucidate the anti-oxidative effect(s) of putrescine (10 mg/kg b.w./day) in rats treated per os with either sodium nitrite (10 mg/kg b.w./day) or normal saline (control) for 14 days. The putrescine was given to rats for 7 days only (days 7-14) and it was introduced 3-4 hrs after nitrite or saline dosage. Sodium nitrite increased thiobarbituric acid reactive substances (TBARS) in rat small intestinal mucosa and liver, and the agent did not have any effect on the total anti-oxidant status (TAS) and lipid peroxidation of rat blood. Nitrite did not also change the activity of superoxide dismutase (SOD) in the small intestinal mucosa, liver and blood, as well. Pretreatment of nitrite-treated rats with putrescine decreased TBARS and increased TAS in animals. Putrescine decreased SOD activity in the blood and liver of nitrite- and/or saline-treated rats, however, the agent did not affect the SOD enzyme in the small intestinal mucosa. Results suggest that putrescine dosed to nitrite-treated rats possesses some anti-oxidative properties.

10

Antioxidant effect of vitamin E on motility, viability and lipid peroxidation of cattle spermatozoa under oxidative stress

58%

Bansal A. K. , Bilaspuri G. S.

Animal Science Papers and Reports

|

2009

|

tom 27

|

nr 1

5-14

EN

Vitamin E is one of the major membrane protectants against reactive oxygen species ( ROS) and lipid peroxidation (LPO). The study aimed at determining the optimum dose of vitamin E to reverse free radical-mediated oxidative damage on motility, viability and LPO of bulls’sperm. Fresh semen of five local crossbred bulls was suspended in 2.9% sodium citrate, divided into equal fractions and subjected to vitamin E treatment (0, 1, 2, 2.5 mM) in the presence or absence of oxidative stress inducer, i.e ferrous ascorbate (FeAA, containing 150 μM FeSO4 and 750 μM ascorbic acid). All sperm suspensions were incubated at 37°C for 2 h. Treatment with FeAA reduced sperm motility and viability, but increased the LPO. All doses of vitamin E increased sperm motility and viability,but reduced LPO. However, 2 mM vitamin E was most effective. In conclusion, vitamin E reduced the LPO caused by FeAA, and improved sperm motility and viability in vitro under induced oxidative stress.

PL

Witamina E jest jednym z głównych czynników chroniących błony komórkowe zarówno przed działaniem rekatywnych form tlenu, jak i przed peroksydacja lipidow (LPO). Celem badań było określenie optymalnej dawki witaminy E , która odwróciłaby szkodliwy wpływ oksydacji – wywołany działaniem wolnych rodników – na żywotność i ruchliwość plemników buhaja oraz na poziom LPO. Świeże nasienie pobrane od pięciu lokalnych mieszańcowych buhajów było zawieszone w 2.9% roztworze cytrynianu sodu, a następnie rozdzielone na równe porcje i poddane działaniu witaminy E (0,1, 2, 2.5mM) w obecności lub braku induktora stresu oksydacyjnego, którym był askorbinian żelazawy (FeAA), zawierający 150 μM FeSo4 i 750μM kwasu askorbinowego. Wszystkie porcje nasienia były inkubowane przez 2 godz. w temperaturze 37oC. Działanie FeAA obniżało żywotność i ruchliwość plemników przy jednoczesnym wzroście LPO. Natomiast wszystkie dawki witaminy E powodowały wzrost żywotności i ruchliwości plemników, a jednocześnie obniżenie poziomu LPO, przy czym najbardziej efektywne okazało się stężenie 2mM witaminy E. Podsumowując, działanie witaminy E obniża poziom peroksydacji lipidów wywołanej działaniem FeAA i podwyższa żywotność i ruchliwość plemników in vitro w warunkach indukowanego stresu oksydacyjnego.

11

Flavonoids - food sources and health benefits

58%

Kozlowska A. , Szostak-Wegierek D.

Roczniki Państwowego Zakładu Higieny

|

2014

|

tom 65

|

nr 2

EN

Flavonoids are a group of bioactive compounds that are extensively found in foodstuffs of plant origin. Their regular consumption is associated with reduced risk of a number of chronic diseases, including cancer, cardiovascular disease (CVD) and neurodegenerative disorders. Flavonoids are classified into subgroups based on their chemical structure: flavanones, flavones, flavonols, flavan-3-ols, anthocyanins and isoflavones. Their actions at the molecular level include antioxidant effects, as well the ability to modulate several key enzymatic pathways. The growing body of scientific evidence indicates that flavonoids play a beneficial role in disease prevention, however further clinical and epidemiological trials are greatly needed. Among dietary sources of flavonoids there are fruits, vegetables, nuts, seeds and spices. Consumption of these substances with diet appears to be safe. It seems that a diet rich in flavonoids is beneficial and its promotion is thus justifiable.

PL

Flawonoidy to grupa związków bioaktywnych występujących powszechnie w żywności pochodzenia roślinnego. Aktualne dane literaturowe wskazują, że substancje te, spożywane wraz z dietą człowieka, wykazują działanie ochronne przed wieloma chorobami przewlekłymi, w tym przed niektórymi nowotworami oraz schorzeniami układu sercowo-naczyniowego, a ponadto pozytywnie wpływają na układ nerwowy. W zależności od struktury chemicznej wyróżnia się takie podklasy flawonoidów jak: flawony, flawanony, flawonole, flawanole, antocyjany i izoflawony. Przypuszcza się, że mechanizm działania tych substancji opiera się na ich silnych właściwościach antyoksydacyjnych oraz innych mechanizmach, takich jak zdolność do modulowania licznych szlaków enzymatycznych. W wielu badaniach wykazano ich korzystne działanie w prewencji chorób przewlekłych. Jednakże poznanie dokładnego metabolizmu tych substancji wymaga prowadzenia dalszych badań. Źródłami flawonoidów w diecie człowieka są warzywa, owoce, orzechy i nasiona, a także niektóre przyprawy. Spożywanie tych substancji wraz z dietą człowieka wydaje się być bezpieczne. Uzasadnionym zatem wydaje się promowanie diety bogatej we flawonoidy.

12

The influence of precursors and elicitor feeding on lignans accumulation in cultures of Taxus × media roots carrying a taxadiene synthase transgene

58%

Wencel A. , Syklowska-Baranek K. , Lysik K. , Pietrosiuk A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2015

|

tom 96

|

nr 1

13