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1

Increased oxidative stress status in rat serum after five minutes treadmill exercise

100%

Trofin F.-P. , Ciobica A. , Cojocaru D. , Chirazi M. , Honceriu C. , Trofin L. , Serban D. , Timofte D. , Cojocaru S. , Anton E.

Open Medicine

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2014

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tom 9

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nr 5

722-728

EN

Although it is accepted that an important correlation exists between the physical exercise and the oxidative stress status, the data regarding the levels of the main oxidative stress markers after physical training have been difficult to interpret and a subject of many controversies. There are also very few studies regarding the effects of short-time exercise on the oxidative stress status modifications. Thus, in the present report we were interested in studying the modifications of some oxidative stress markers (two antioxidant enzymes-superoxide dismutase and glutathione peroxidase, a lipid peroxidation parameter - malondyaldehide, the total antioxidant status and protein carbonyl levels), from the serum of rats that were subject to one bout of five minutes exercise on a treadmill, when compared to a control sedentary group. In this way, we observed a decrease of superoxide dismutase specific activity in the rats which performed the exercises. Still, no modifications of glutathione peroxidase specific activity were found between groups. In addition, increased levels of malondyaldehide and protein carbonyls were observed in the rats subjected to exercises. In conclusion, our data provides new evidence regarding the increase of the oxidative stress status, as a result of a 5-minutes bout of treadmill exercising in rats, expressed through a decrease in the SOD specific activity and the total antioxidant status and also an increase of the lipid peroxidation and protein oxidation processes.

2

Rat skin as an experimental model in medicine

100%

Niczyporuk M.

Progress in Health Sciences

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2018

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tom 8(2)

223-228

EN

Scientific experiments due to safety and ethical limitations regarding research human are often based on animal models. Rats are laboratory animals which are commonly used for these purposes. It should be remembered that morphologi- cal and functional differences between rat skin and human skin may refer to the correct interpretation of scientific results.

3

Is Ozone (O3) Treatment Effective in Atrophic Rhinitis? Experimental Animal Study

88%

Altaş B. , Koçak H. E. , Altınay S. , Yücebaş K. , Taşkın Ü. , Oktay M. F.

Otolaryngologia Polska

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2018

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tom 72(5)

37-44

EN

Introduction: Atrophic rhinitis (AR) is a disease characterized by atrophy of the mucosa, submucosa, bone tissue due to an unknown cause and excessive nasal cavity enlargement. The disease still has no complete treatment, and the treatment of functional loss of atrophic cells in AR is still a matter to be investigated. Ozone (O3) therapy has been shown to enhance cell metabolism, angiogenesis, fibroblast activity and collagen synthesis. Aim: To determine whether ozone treatment affects the disease histopathologically, in experimentally created AR. Material - Method: Twelve Wistar Hanover strain albino male rats were included in the study. Atrophic rhinitis was induced in animals by administering Pasteurella multocida toxin diluted with saline for 21 days to both nasal cavities. A total of 12 animals included in the study were divided into 2 groups as control and study. Ozone gas (60 μg/mL) was administered rectally to the study group for 21 days. After 2 weeks, the rats were decapitated, the nasal cavities were removed as a block, and atrophic rhinitis parameters (epithelial hyperplasia, goblet cell loss, cilia loss, inflammatory infiltration and vascular ectasia) were evaluated under light microscopy by histopathological examination and statistically interpreted. Result: The incidence of vascular ectasia was significantly lower in the ozone group compared to the control group (p<0.05). There was no significant difference between the groups regarding other histopathologic findings. Conclusion: Ozone treatment was moderate at the histopathological level. We concluded that ozone therapy has no or very limited effect on atrophic rhinitis.

4

Myocardial remodeling in rats with metabolic syndrome: role of Rho-kinase mediated insulin resistance

88%

Li C.-B. , Li X.-X. , Chen Y.-G. , Gao H.-Q. , Bao C.-M. , Liu X.-Q. , Bu P.-L. , Zhang J. , Zhang Y. , Ji X.-P.

Acta Biochimica Polonica

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2012

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tom 59

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nr 2

249-254

EN

Insulin resistance (IR) plays a critical role in metabolic syndrome (MS). Previous studies have demonstrated that activated ROCK is increased in MS patients. However, the effect of Rho-kinase (ROCK) on IR has not been definitely determined. Thus, the aims of the present study were to determine whether ROCK activation induces IR or affects myocardial structure and function, as well as the possible mechanisms underlying this process. Wistar rats fed high fat, high glucose and high salt diet sewed as model of MS and we used transmission electron microscopy, echocardiogram technology, and terminal deoxynucleotidyl transferase-mediated DNA nick-end labeling staining to identify any myocardial damage. The protein levels of MYPT-1 (characteristic of ROCK activation), IRS-1 and AKT were analyzed by immunohistochemistry and Western blotting. In hearts from MS rats, we found increased protein levels of phospho-MYPT-1 and phospho-IRS-1 (Ser307) and decreased phospho-AKT compared to levels in normal rats. In conclusion, the results suggest that ROCK-mediated IR is involved in the development of myocardial impairments in MS rats and that this effect is mediated probably via the IRS-1/PI3-kinase/AKT pathway.

5

Light-dark dependence of electrocardiographic changes during asphyxia and reoxygenation in a rat model

88%

Bačová I. , Švorc P. , Kundrík M. , Fulton B.

Open Medicine

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2011

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tom 6

|

nr 1

89-97

EN

The aim of this study was to evaluate the effect of ventilation on electrocardiographic time intervals as a function of the light-dark (LD) cycle in an in vivo rat model. RR, PQ, QT and QTc intervals were measured in female Wistar rats anaesthetized with both ketamine and xylazine (100 mg/15 mg/kg, i.m., open chest experiments) after adaptation to the LD cycle (12:12h) for 4 weeks. Electrocardiograms (ECG) were recorded before surgical interventions; after tracheotomy, and thoracotomy, and 5 minutes of stabilization with artificial ventilation; 30, 60, 90 and 120 seconds after the onset of apnoea; and after 5, 10, 15, and 20 minutes of artificial reoxygenation. Time intervals in intact animals showed significant LD differences, except in the QT interval. The initial significant (p<0,001) LD differences in PQ interval and loss of dependence on LD cycle in the QT interval were preserved during short-term apnoea-induced asphyxia (30–60 sec) In contrast, long-term asphyxia (90–120 sec) eliminated LD dependence in the PQ interval, but significant LD differences were shown in the QT interval. Apnoea completely abolished LD differences in the RR interval. Reoxygenation restored the PQ and QT intervals to the pre-asphyxic LD differences, but with the RR intervals, the LD differences were eliminated. We have concluded that myocardial vulnerability is dependent on the LD cycle and on changes of pulmonary ventilation.

6

Is Ozone (O3) Treatment Effective in Atrophic Rhinitis? Experimental Animal Study

88%

Altaş B. , Koçak H. E. , Altınay S. , Yücebaş K. , Taşkın Ü. , Oktay M. F.

Polish Journal of Otolaryngology

|

2018

|

tom 72

|

nr 5

37-44

EN

Introduction: Atrophic rhinitis (AR) is a disease characterized by atrophy of the mucosa, submucosa, bone tissue due to an unknown cause and excessive nasal cavity enlargement. The disease still has no complete treatment, and the treatment of functional loss of atrophic cells in AR is still a matter to be investigated. Ozone (O3) therapy has been shown to enhance cell metabolism, angiogenesis, fibroblast activity and collagen synthesis. Aim: To determine whether ozone treatment affects the disease histopathologically, in experimentally created AR. Material - Method: Twelve Wistar Hanover strain albino male rats were included in the study. Atrophic rhinitis was induced in animals by administering Pasteurella multocida toxin diluted with saline for 21 days to both nasal cavities. A total of 12 animals included in the study were divided into 2 groups as control and study. Ozone gas (60 μg/mL) was administered rectally to the study group for 21 days. After 2 weeks, the rats were decapitated, the nasal cavities were removed as a block, and atrophic rhinitis parameters (epithelial hyperplasia, goblet cell loss, cilia loss, inflammatory infiltration and vascular ectasia) were evaluated under light microscopy by histopathological examination and statistically interpreted. Result: The incidence of vascular ectasia was significantly lower in the ozone group compared to the control group (p<0.05). There was no significant difference between the groups regarding other histopathologic findings. Conclusion: Ozone treatment was moderate at the histopathological level. We concluded that ozone therapy has no or very limited effect on atrophic rhinitis.

7

Light-dark dependence of electrocardiographic changes during asphyxia and reoxygenation in a rat model

88%

Bačová I. , Švorc P. , Kundrík M. , Fulton B.

Open Medicine

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2010

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tom 5

|

nr 5

611-619

EN

The aim of this study was to evaluate the effect of ventilation on electrocardiographic time intervals as a function of the light-dark (LD) cycle in an in vivo rat model. RR, PQ, QT and QTc intervals were measured in female Wistar rats anaesthetized with both ketamine and xylazine (100 mg/15 mg/kg, i.m., open chest experiments) after adaptation to the LD cycle (12:12h) for 4 weeks. Electrocardiograms (ECG) were recorded before surgical interventions; after tracheotomy, and thoracotomy, and 5 minutes of stabilization with artificial ventilation; 30, 60, 90 and 120 seconds after the onset of apnoea; and after 5, 10, 15, and 20 minutes of artificial reoxygenation. Time intervals in intact animals showed significant LD differences, except in the QT interval. The initial significant (p<0,001) LD differences in PQ interval and loss of dependence on LD cycle in the QT interval were preserved during short-term apnoea-induced asphyxia (30–60 sec) In contrast, long-term asphyxia (90–120 sec) eliminated LD dependence in the PQ interval, but significant LD differences were shown in the QT interval. Apnoea completely abolished LD differences in the RR interval. Reoxygenation restored the PQ and QT intervals to the pre-asphyxic LD differences, but with the RR intervals, the LD differences were eliminated. We have concluded that myocardial vulnerability is dependent on the LD cycle and on changes of pulmonary ventilation.

8

75%

Lazarov N. , Itzev D. , Usunoff K. , Negrev N. , Radomirov R.

Open Medicine

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2010

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tom 5

|

nr 3

358-364

EN

The recto-anal region is innervated by extrinsic and intrinsic nerves and a number of neuropeptides including substance P (SP) have been suggested to participate in the regulation of intestinal movements. We examined the age-related changes in the distribution of SP-immunoreactive nerve structures in the distal part of the rat large intestine. Using immunohistochemistry, the presence of SP was studied in fresh tissues from Wistar rats at different ages taken at three sampling sites, the distal rectum, anal canal and internal anal sphincter. In the 15-day old rats the myenteric plexus of the distal rectum and anal canal was well outlined by numerous SP-immunoreactive varicose nerve fibres encircling immunonegative perikarya. In the circular muscle layer, nerve fibres and small nerve bundles ran parallel to the muscle cells, while in the longitudinal muscle layer, only occasional nerve fibres were seen. At the level of the internal anal sphincter, no myenteric ganglia were present. Here, thin varicose fibers ran parallel to the smooth muscle cells. In the 3-month old rats, a larger number of intensely staining SP-immunoreactive nerve fibres were found and in the circular muscle layer, thicker nerve strands were observed. In the 26-month old rats, the density and staining intensity of SP-immunopositive nerve fibres in the myenteric plexus was lower than in the 3-month-old rats. Similar changes in the SP-immunostained fibres in the internal anal sphincter were observed. Degenerative alterations in SP-containing fibres during aging appear to play a role in ano-rectal motility and sphincter control.

9

Założenia generalne dotyczące aktu przełożonego kościelnego wymagającego współudziału organów partycypacji (kan. 127§ 1 KPK)

44%

Dzierżon G.

Studia Redemptorystowskie

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2013

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nr 11

371-381

EN

The author of the presented article out a thorough interpretation of can. 127 § 1 CIC. According to the analyses, in the final stage of the decision process a superior remains autonomous in placing acts. However, making the decision becomes impossible without the consent of a college or group of persons. The author of the study proved that convoking a college or group of persons is a strict requirement. This is because in this case a disabling act applies in implicit manner. The analysed can. 127 § 1 CIC include different dispositions concerning the consent and counsel. If a superior does not procure the consent, he cannot act as he intended to. As for the counsel, he is obliged under sanction of invalidity to seek the judgement of college or group of persons. In this case, however, it is not necessary for him to follow the opinion expressed by the consultative body. According to the author, the diversity of the solutions applied from the diverse character of acts placed by an ecclesiastical superior in the canonical system.