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Immobilization of Catalase via Adsorption onto Natural and Modified Montmorillanit Analsim-Clay

100%

Alkan S. , Gür T. , Gür A. , Uruc H. , Demir H.

2009

tom Vol. 83, nr 12

2089-2095

Immobilization of the enzyme catalase on natural and modified montmorillanit analsimclays was investigated using a batch system. Such an immobilization does not result in enzyme in activation and constitutes a valuable method for immobilizing catalase at high ionic strength. In the immobilization process, the effect of pH, ionic strength and reaction temperature were chosen as parameters. Further more the optimization of immobilization conditions were studied using data obtained from experimental results. For the free catalase and three different immobilized catalase enzymes, the optimum pH values 8, 7, 7 and 8; reaction temperature 30°C; ionic strength 0.25 M were found. It was determined that enzyme activity for enzyme supported by natural clay was 73.3 percent, when it was retained during the storage at 4°C for a period of 60 days. It was observed that storage and operational stabilities of the enzyme in creased with immobilization. The results obtained show that montmorillanit analsim-clay is valuable and favour able support the simple adsorption of enzymes.

Adsorption of crystal violet from aqueous solution on activated carbon derived from Gölbaşi lignite

86%

Depci T. , Kul A. R. , Onal Y. , Disli E. , Alkan S. , Turkmenoglu Z. F.

2012

tom Vol. 48, iss. 1

253-270

Activated carbon (AC) was obtained from lignite of the local resource, Gölbaşi - Adiyaman (Turkey) by chemical activation. The Gölbaşi lignite was chosen as the precursor for its availability and low cost. The BET surface area of the activated carbon was found 921 m2/g. The AC was used as an adsorbent for Crystal Violet (CV) in aqueous solution. The adsorption properties of CV onto the activated carbon are discussed in terms of the adsorption isotherms (Langmuir and Freundlich) and the kinetic models (pseudo-first-order, pseudo-second-order and intraparticle diffusion model). It was shown that the experimental results best fitted by the Langmuir model, and the second-order kinetic equation. The thermodynamic parameters show that the adsorption process is endothermic. The experimental results point out that the obtained activated carbon is a viable candidate for sorbent removing CV from aqueous solutions.