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1 Acta Biologica Cracoviensia. Series Botanica

1 2003

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Temporal and spatial gene expression of Ole e 3 allergen in olive [Olea europaea L.] pollen

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De D. A. J. , Cismondi I. A. , Castro A. J. , Hamman-Khalifa A. M. , Rodriguez-Garcia M. I.

Acta Biologica Cracoviensia. Series Botanica

2003

tom 45

nr 1

This paper describes the distribution of Ole e 3 allergen and its transcripts in the developing anther and in mature olive pollen. Northern blot and RT-PCR analyses over the course of pollen development show that Ole e 3 transcripts accumulate exclusively in mature pollen. This gene therefore corresponds to a "late gene." The sequences amplified by RT-PCR display high identity with those already reported for Ole e 3, including two Ca2+-binding motifs. Immunoblot analysis of the allergen shows that Ole e 3 accumulates during the same stage as the corresponding transcripts, suggesting a transcriptional regulation mechanism for the expression of the gene. Finally, the use of transmission electron microscopy techniques has shown that (a) the allergen is located mainly in the vicinity of membrane systems and in the aperture regions of the mature pollen grain, and (b) Ole e 3 transcripts are detectable after using in situ RT-PCR. These data are significant clues to the biological roles of the protein in olive pollen biology. Such putative functions are discussed.