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Blastocytoza u 5-letniego chłopca – opis przypadku

100%

Pawłowska J. , Pawłowska-Iwanicka K. , Stelmach I.

Pediatria i Medycyna Rodzinna

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2018

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tom 14

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nr 3

324-326

EN

Blastocystis hominis is one of the most common parasites present in the human gastrointestinal tract. Transmission usually occurs via food and water contaminated with cystic forms or via the faecal-oral route. The prevalence of infection is approximately 30–50% in developing countries and about 1.5–10% in developed ones. Blastocystis hominis was long considered as a large intestine commensal due to asymptomatic infestation, possibly characterised by temporary or permanent gastrointestinal carrier state, in some cases. Currently, this protozoan is considered pathogenic as symptoms develop in the course of infestation, especially in infected immunocompromised individuals. The importance of Blastocystis hominis as a factor responsible for enteral and parenteral symptoms is underestimated in clinical practice, and the infestation with this parasite is underdiagnosed. We present a case of a 5-year-old boy infected with Blastocystis hominis, who developed gastrointestinal symptoms and urticaria.

PL

Blastocystis hominis to jeden z najczęściej stwierdzanych pierwotniaków bytujących w przewodzie pokarmowym człowieka. Do zarażenia człowieka dochodzi najczęściej drogą fekalno-oralną lub pokarmową przez zanieczyszczony cystami pierwotniaka pokarm lub wodę. Częstość występowania Blastocystis hominis w krajach rozwijających się wynosi około 30–50%, zaś w krajach rozwiniętych – w granicach 1,5–10%. Przez długi okres Blastocystis hominis uznawany był za komensala jelita grubego, ponieważ w niektórych przypadkach inwazja może przebiegać asymptomatycznie, a cechować ją może przejściowe lub trwałe nosicielstwo w przewodzie pokarmowym. Obecnie jednak pierwotniak uznawany jest za gatunek pasożytniczy, gdyż objawy występują zwłaszcza u osób z obniżoną odpornością, zarażonych tym patogenem. Znaczenie Blastocystis hominis jako czynnika odpowiedzialnego za objawy jelitowe i pozajelitowe jest niedoszacowane w praktyce klinicznej, a zarażenie tym pasożytem nie jest w pełni diagnozowane. W pracy przedstawiono przypadek 5-letniego chłopca, u którego zarażenie Blastocystis hominis wywołało objawy ze strony przewodu pokarmowego oraz pokrzywkę.

2

Obesity, lipid profiles and oxidative stress in children after liver transplantation

88%

Czubkowski P. , Wierzbicka A. , Pawłowska J. , Jankowska I. , Socha P.

Acta Biochimica Polonica

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2017

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tom 64

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nr 4

661-665

EN

Purpose. In adult liver transplant recipients, coronary artery disease and congestive heart failure are significant cause of morbidity and mortality. This may be attributed to the long-term immunosuppressive treatment, mostly with calcineurin inhibitors and steroids, which in long-term may be associated with hyperlipidemia, oxidative stress and cardiovascular complications. Since such data for children is sparse, the aim of this study was to assess the lipid and oxidative stress markers after pediatric liver transplantation (LTx). Method. We performed prospective analysis of 74 children, at the median age of 7.9 (2.8-11.6) years, 3.2 (1.2-4.3) years after LTx. We assessed the BMI Z-scores, cholesterol fractions (LDLc, HDLc, VLDLc), triglicerides, apolipoproteins (ApoAI, ApoB, ApoE), LCAT, insulin resistance by HOMA-IR and markers of oxidative stress and atherosclerosis: glutathione (GSH), glutathione peroxidase (GPx), asymmetrical dimethyl arginine (ADMA) and oxidized low-density lipoprotein (oxyLDL). At baseline, the results were compared with a healthy age-and-sex matched control group. After 3.1±0.3 year follow-up we repeated all investigations and compared them with the baseline results. RESULTS. At the baseline, we investigated 74 patients 3.2 (1.2-4.3) years after LTx, at the median age of 7.9 (2.8-11.6) years. The prevalence of overweight or obesity (BMI >85th percentile) was 23% and was more common in girls (24% vs 20%). Fourteen patients had TCH >200 mg%, 9 patients had LDLc >130 mg% and TG were at normal levels in all patients. Compared to the controls, there were no significant differences in lipid profiles but we found decreased GSH (p<0.001) and GPx (p<0.001) which play role as an antioxidant defense. OS markers were higher in the study group: ADMA (p<0.001), and oxyLDL (p<0.0001). Insulin resistance by HOMA-IR was increased in the study group (p=0.0002) but fasting glucose remained within normal ranges in all patients. After 3.1-year follow-up, the BMI >95th and >85Th percentile was present in 8% and 14% respectively. ADMA and oxyLDL decreased, whilst GSH and GPx increased when compared to the baseline. There was also significant decrease in apoB and Lp(a). Conclusion. Children after LTx had normal lipid profiles when compared to controls, however there is a tendency for hypercholesterolemia and obesity, which may play a role in cardiovascular complications in the future. Some markers of oxidative stress were increased after LTx, however further investigations are required to establish its clinical significance.

3

Normal levels of serum pancreatic enzymes in patients with progressive familial intrahepatic cholestasis type 2

75%

Walkowiak J. , Jankowska I. , Lisowska A. , Biczysko M. , Wierzbicka A. , Pawłowska J.

Acta Biochimica Polonica

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2010

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tom 57

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nr 4

573-575

EN

Introduction. High prevalence of elevated serum pancreatic enzymes in children with cholestasis with normal fecal elastase-1 concentrations has been documented. However, this state is related predominantly to biliary atresia. Therefore, we aimed to assess pancreatic damage by measuring serum pancreatic enzymes in patients with progressive familial intrahepatic cholestasis type 2 (PFIC type 2). Materials and methods. Twenty PFIC type 2 patients with normal serum bilirubin and bile acid concentrations were included in the study. Thirty pancreatic insufficient cystic fibrosis (PI-CF) patients, thirty patients with acute pancreatitis (AP) and thirty healthy subjects (HS) served for the purpose of comparison. In all subjects, serum lipase and elastase-1 levels were measured. Results. In all but one PFIC type 2 patients and all HS normal lipase activities were found. Serum elastase-1 concentrations were normal in all PFIC type 2 patients and HS. The enzyme levels were very similar in both groups studied. Lipase activities in PFIC type 2 patients were significantly higher than in PI-CF patients (p < 0.00001) and lower than in patients with AP (p < 0.00001). Serum elastase-1 levels in PFIC type 2 patients were significantly lower than in patients with AP (p < 0.00001) and not different from those in PI-CF patients. In conclusion. serum pancreatic enzymes in patients with PFIC type 2 are normal. No pancreatic damage in these patients could be detected.

4

Small intestinal bacterial overgrowth in patients with progressive familial intrahepatic cholestasis

75%

Lisowska A. , Kobelska-Dubiel N. , Jankowska I. , Pawłowska J. , Moczko J. , Walkowiak J.

Acta Biochimica Polonica

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2014

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tom 61

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nr 1

103-107

EN

Background & Aims: To date, no studies concerning the presence of small intestinal bacterial overgrowth in patients with progressive familial intrahepatic cholestasis were published. Based upon characteristic of progressive familial intrahepatic cholestasis one can expect the coexistence of small intestinal bacterial overgrowth. The aim of the study was to assess the incidence of small intestinal bacterial overgrowth in patients with progressive familial intrahepatic cholestasis. Methods: 26 patients aged 8 to 25 years with progressive familial intrahepatic cholestasis were included in the study. Molecular analysis of ABCB11 gene was performed in the vast majority of patients. In all patients Z-score for body weight and height, biochemical tests (bilirubin, bile acid concentration, fecal fat excretion) were assessed. In all patients hydrogen-methane breath test was performed. Results: On the basis of first hydrogen-methane breath test, diagnosis of small intestinal bacterial overgrowth was confirmed in 9 patients (35%), 5 patients (19%) had borderline results. The second breath test was performed in 10 patients: in 3 patients results were still positive and 2 patients had a borderline result. The third breath test was conducted in 2 patients and positive results were still observed. Statistical analysis did not reveal any significant correlations between clinical, biochemical and therapeutic parameters in patients with progressive familial intrahepatic cholestasis and coexistence of small intestinal bacterial overgrowth. Conclusions: Our results suggest that small intestinal bacterial overgrowth is frequent in patients with progressive familial intrahepatic cholestasis. Moreover, it seems that this condition has the tendency to persist or recur, despite the treatment.