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EN
Pore structure and mineral matrix elastic moduli are indispensable in rock physics models. We propose an estimation method of pore structure and mineral moduli based on Kuster-Toksöz model and Biot’s coefficient. In this technique, pore aspect ratios of five different scales from 100 to 10-4 are considered, Biot’s coefficient is used to determine bounds of mineral moduli, and an estimation procedure combined with simulated annealing (SA) algorithm to handle real logs or laboratory measurements is developed. The proposed method is applied to parameter estimations on 28 sandstone samples, the properties of which have been measured in lab. The water saturated data are used for estimating pore structure and mineral moduli, and the oil saturated data are used for testing these estimated parameters through fluid substitution in Kuster–Toksöz model. We then compare fluid substitution results with lab measurements and find that relative errors of P-wave and S-wave velocities are all less than 5%, which indicates that the estimation results are accurate.
EN
Optically stimulated luminescence (OSL) dating was applied to sediment samples in order to provide age constraints on the palaeo-earthquake events that are recorded in a trench on the Lingwu fault near Yinchuan on the northeastern margin of the Tibetan Plateau, China. Combining seventeen OSL ages with field observations in the trench shows that at least four events took place at this specif-ic site, and that this fault has been active over the past 200 ka.
EN
Dendrobium huoshanense has long been used to treat various diseases in oriental medicine. In order to study its gene expression profile, transcripts involved in the biosynthesis of precursors of polysaccharides, as well as mechanisms underlining morphological differences between wild and cultivated plants, three organs of both wild type and cultivated D. huoshanense were collected and sequenced by Illumina HiSeq4000 platform, yielding 919,409,540 raw reads in FASTQ format. After Trinity de novo assembly and quality control, 241,242 nonredundant contigs with the average length of 967.5 bp were generated. qRT-PCR experiment on the selected transcripts showed the transcriptomic data were reliable. BLASTx was conducted against NR, SwissProt, String, Pfam, and KEGG. Gene ontology annotation revealed more than 40,000 contigs assigned to catalytic activity and metabolic process, suggesting its dynamic physiological activities. By searching KEGG pathway, six genes potentially involved in mannose biosynthetic pathway were retrieved. Gene expression analysis for stems between wild and cultivated D. huoshanense resulted in 956 genes differentially expressed. Simple sequence repeats (SSRs) analysis revealed 143 SSRs with the unit size of 4 and 3,437 SSRs the size of 3. The obtained SSRs are the potential molecular markers for discriminating distinct cultivars of D. huoshanense.
EN
The main aim of this study was to screen and identify cadmium-resistant fungus and to characterize its cadmium adsorption. A cadmium-resistant strain (CN35) was isolated from cadmium-polluted paddy soil. Based on morphlogical characteristics, internal transcribed spacers region and β-tubulin gene sequence phylogenesis analysis, the strain was preliminarily identified to be Penicillium sp. This strain was resistant to Cd at 45 mM with Cd adsorption rate up to 83.56%, and also resistant to other heavy metals such as Pb, Zn, and Cu. When Cd²⁺ concentration ranged from 2 to 5 mM, the fungal colony changed from yellow/green to red. The colony morphology was also affected by Cd²⁺ concentrations with protuberances forming on the colony surface at 20 mM. The strain CN35 was found to grow well at pH 4 to 8 at between 24ºC and 37ºC, and the optimal growth conditions were established to be at pH 4 and 30ºC. Fermented liquid of the strain is neither disease-causing nor inhibitory to rice seedling emergence, but rather improves rice seedling and root growth and enhances rice detoxification ability under Cd stress. Thus, the Cd-resistant fungus CN35 has the potential to treat Cd-polluted rice paddies.
EN
The WUSCHEL-related homeobox (WOX) gene family is a hotspot for diverse functions in development biology. Recently available whole-genome sequences allowed a more comprehensive analysis of WOX genes in watermelon (Citrullus lanatus). The results of this study provide a genomic framework for further research of watermelon WOX genes and contribute to understanding of the evolutionary mode of WOX genes in Cucurbitaceae crops. The qRT-PCR analysis demonstrated active expression of 11 WOX genes in watermelon tissues, which brings new evidence for WOX genes acting as conserved factors during watermelon development. Moreover, the distinct expression profiles of WOX genes during shoot initiation might lead todifferent shoot regeneration abilities. This work gives an overview of the differentially expressed WOX genes during shoot regeneration inwatermelon. The interrelations of WOX genes, phytohormones and other transcription factors during the process will be the focus of future studies.
EN
Hydroponics experiment was conducted to identify cadmium (Cd) tolerance and phytoextraction potential of moso bamboo (Phyllostachys pubescens) seedlings grown under different levels of Cd. Application of Cd adversely affected the overall growth and ultrastructural characteristics of moso bamboo. At the highest Cd concentration (400 lM), the growth of moso bamboo seedlings was significantly inhibited, and Cd concentrations in leaves, stems and roots reached the maximum of 25.6, 129.8 and 377 mg kg-1, respectively. Scanning electron microscopy (SEM) revealed that the excessive Cd concentrations caused formation of abundant inclusions in the root and stem. The ultrastructural analysis using transmission electron microscopy (TEM) showed that the excessive Cd concentrations caused abnormal-shaped chloroplasts, disappearance of endoplasmic reticulum, and shrinkage of nucleus and loss of thylakoid membranes. It is suggested that use of moso bamboo as experimental material provides a new perspective for remediation of heavy metal-contaminated soil due to its deep root system and larger biomass. However, mechanisms of Cd uptake and accumulation as well as metal interactions within the plant cell need to be investigated further.
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