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1

Canine skin tumours with adnexal differentiation: histopathology and immunohistochemistry

100%

Jasik A. , Kozaczynski W. , Reichert M.

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nr 2

277-283

EN

Thirty nine canine adnexal tumours were histologically and immunohistochemically analysed. They were classified as trichoblastomas, trichoepitheliomas, sebaceous adenomas and epitheliomas, hepatoid gland adenomas and epitheliomas, and aporcine adenocarcinomas. Moreover, hepatoid gland angioadenoma and angioepithelioma were recognised. Studies of follicular tumours revealed coexpression of cytokeratin MNF116 and LP34, but sebaceous and hepatoid gland tumours as well as apocrine adenocarcinomas showed differences in the expression of both cytokeratins. All the tumours were negative for vimentin except two hepatoid gland adenomas in which coexpression of vimentin and cytokeratin was observed. Positive reaction for neuron-specific enolase (NSE) was observed in follicular tumours, whereas expression of α-smooth muscle actin (α-SMA) was found only in apocrine adenocarcinomas. Moreover, the presence of NSE observed in hepatoid gland adenomas was considered as non-specific just as both glial fibrillary acidic protein in sebaceous gland tumours and hepatoid gland adenomas, and also S100 protein in sebaceous adenomas. All the examined tumours showed lack of expression of both desmin and factor FVIII. Thus, among the used antibodies, cytokeratins, vimentin, NSE, and α-SMA play a main role in the evaluation of canine adnexal histogenesis.

2

Influence of selected technological treatments on the BLV provirus DNA occurring in the tissues of leukaemic cattle

88%

Reichert M. , Grundboeck-Jusko J. , Rulka J. , Stec J. , Kozaczynski W.

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nr 2

3

Przypadek zespolu skorno-nerkowego w wielkotowarowej fermie swin

75%

Pejsak Z. , Kolodziejczyk P. , Kozaczynski W. , Stadejek T. , Lipowski A. , Roszkowski J.

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nr 08

591-594

4

Wystepowanie poodsadzeniowego wielonarzadowego zespolu wyniszczajacego w krajowej populacji swin

75%

Podgorska K. , Stadejek T. , Szczotka A. , Kozaczynski W. , Porowski M. , Pejsak Z.

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nr 05

330-333

EN

The aim of the study was to assess the occurrence of the postweaning wasting syndrome (PMWS) in Polish pig herds. Thirty-two herds were selected from 195 visited pig production sites based on the appearance of clinical lesions. The population of breeding herds ranged from 25 to 2000 sows and the percentage of losses varied from 2 to 20%. In each farm 3 pigs affected by wasting were autopsied and lymph nodes were collected. Sections of lymph nodes were examined for the presence of histopathological lesions and tested with in situ hybridization to detect the DNA of porcine circovirus type 2 (PCV2), an etiological agent of PMWS. The results were considered as positive when characteristic histopathological changes were detected along with an abundant amount of viral DNA. PMWS was recognized in 26 pigs (27.9%) from 16 farms (50%). In two of those farms PMWS was diagnosed as sporadic, based on the analysis of production parameters and low rate of losses. In 12 herds (37.5%) no pig had DNA of PCV2 in lymph nodes, while in the remaining 4 herds (12.5%) low amounts of DNA were detected in lymph nodes from at least one pig. PMWS was confirmed in all tested farms with breeding herds of more than 1000 sows, while none of the small farms with breeding herds of less than 100 sows were affected. In two other groups of farms counting 100-499 and 500-1000 sows the percentage of affected herds was respectively 33.3% and 57.1%. The established rate of PMWS occurrence was similar to that claimed by most other European countries. Results obtained in this study suggest that the incidence of PMWS outbreaks increases with the size of the herd. A higher number of properly selected samples markedly enhances the probability of disease detection.

5

The effect of genetically modified maize (MON 810) and soyabean meal (Roundup Ready) on rearing performance and transfer of transgenic DNA to calf tissues

63%

Furgal-Dzierzuk I. , Strzetelski J. , Kwiatek K. , Twardowska M. , Mazur M. , Sieradzki Z. , Kozaczynski W. , Reichert M.

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tom 23

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nr 1

EN

The experiment was performed to determine whether genetically modified maize (MON 810) and soyabean meal (Roundup Ready, MON 40-3-2) used as the main source of feed in a concentrate can affect the performance parameters, basal chemical composition of the musculus thoracis (MT), fatty-acid composition of intramuscular fat, and transfer of transgenic DNA (tDNA) to calf tissues, as well as affect the results of histological examination of calf organs and tissues. In the experiment, forty Polish Black-and-White HF bulls aged 10 days were allocated to 4 groups fed non-modified (traditional) maize and soyabean meal (group TMG/TS), non-modified maize and GM soyabean (group TMG/MS), GM maize and non-modified soyabean meal (group MMG/TS), or GM maize and GM soyabean meal (group MMG/MS). The experiment was terminated at the age of 90 days. Calves were housed in individual pens and fed ad libitum diets with balanced energy and protein according to the IZ-INRA (2009) system. All mixtures contained similar amounts (%) of maize (56), soyabean meal (25), oat (15), premix and limestone (1). There were no major differences in the feed value of Bt maize and RR soyabean meal and their non-modified isogenic counterparts and feed mixtures. There were no effects of GM components on final liveweight, average daily weight gain, MT chemical composition, or fatty-acid profile of intramuscular fat. The calf rumen fluid contained tDNA, but there was no tDNA in the intestinal content, blood, studied organs, or meat. Histological examination of the investigated organs and muscles found no differences among treatments.