Wyniki wyszukiwania - Biblioteka Nauki

1

Performance of classification methods in a microarray setting: a simulation study

100%

Van Sanden S. , Lin D. , Burzykowski T.

Biocybernetics and Biomedical Engineering

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2007

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tom Vol. 27, no. 3

15-28

EN

Dudoit et al., Lee et al., and Statnikov et al. investigated the performance of several classification methods applied to real-life microarray data. Due to the availability of only a few datasets, only a limited number of settings could be evaluated. Also, the true classification and the set of truly differentially expressed genes were unknown. In order to overcome these limitations, a simulation study was conducted, by using a linear mixed effects model to simulate microarray data under different scenarios. Several classification methods were compared with respect to their ability to discriminate between two classes of biological samples in various experimental settings.

2

Excess iron alters the fatty acid composition of chloroplast membrane and decreases the photosynthesis rate: a study in hydroponic pea seedlings

88%

Xu S. , Lin D. , Sun H. , Yang X. , Zhang X.

Acta Physiologiae Plantarum

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2015

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tom 37

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nr 10

EN

As an essential micronutrient, iron (Fe) is directly involved in several fundamental processes in the photosynthetic cells. However, it is not clear if photosynthetic traits affected by high ferrous level are associated with changes in fatty acid composition in chloroplast membranes. To accomplish this, the effects of excess Fe2+ on the fatty acid composition and the fluidity properties of the chloroplast membrane, photosynthesis rate and the chlorophyll fluorescence were investigated in pea (Pisum sativum L.) seedlings grown hydroponically in nutrient solutions with 100, 200, 400 and 600 lM Fe2+ supplied as FeSO4. Increased fluidity of the chloroplast membranes was found under higher Fe2+ treatments, and this might be attributed to the increase in relative content of unsaturated fatty acids (USFA). Excess Fe2+ decreased the chlorophyll content and the electron transport rate, deactivated reaction center of photosystem II, and declined plant net photosynthetic rate. Finally, the reduced plant dry weight was observed. The results indicate that the effects of excess Fe on photosynthesis and fluidity of chloroplast membrane depend on the stress strength and duration, and the increased fluidity of chloroplast membrane may be critical in maintenance of cellular integrity under excess but not lethiferous Fe2+ treatment.

3

Effects of iletamine-zolazepam-xylazine-tramadol combination on biochemical and haematological parameters in cats

75%

Li L. , Dong J. , Lu D. , Jiang S. , Lin D. , Fan H.

Bulletin of the Veterinary Institute in Puławy

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2012

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tom 56

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nr 3

EN

Cats were injected with 2.4 mg/kg of tiletamine-zolazepam, 0.36 mg/kg of xylazine, and 0.8 mg/kg of tramadol, then blood was collected from their peripheral veins at 0.5, 1, 4, 8, 24, 48, 72, and 168 h after the injection. Before injection, the same cats were used as control group (0 h). Biochemical and haematological parameters were measured. The results revealed that the combination of the compounds produced good immobilisation, with minimal changes over time in biochemical and haematological parameters, which were within biologically acceptable limits.

4

Ability of three Pleurotus species for effective use of giant grass compost

75%

Niyimbabazi O. , Nsanzinshuti A. , Hatungimana M. , Lin H. , Zhang L. , Lin D. , Zhanxi L.

Journal of Horticultural Research

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2022

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tom 30

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nr 1

5

Seroepidemiology of Toxoplasma gondii in pet dogs and cats in Beijing, China

75%

Yu J. , Ding J. , Xia Z. , Lin D. , Li Y. , Jia J. , Liu Q.

Acta Parasitologica

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2008

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tom 53

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nr 3

EN

Sera from 534 pet dogs and 335 pet cats from Beijing (China) were tested for anti-Toxoplasma gondii antibodies using an enzyme-linked immunosorbent assay or the latex agglutination test. The seropositivity by year, season, sex and age was analysed. Overall, 128 dogs (24.0%) and 50 cats (14.9%) had antibodies to T. gondii. When analysed by season, the highest seroprevalence was found in spring for dogs (31.3%) and cats (25.1%), and the differences in seroprevalence by season was statistically significant in cats (P<0.01) but not in dogs. The seroprevalence in male dogs (23.7%) and cats (15.1%) were slightly higher than their female counterparts (18.0% in dogs and 12.3% in cats). There was no obvious pattern of seropositivity or significant difference in different age groups in dogs or cats; nonetheless, a high proportion of dogs at 4 years of age were positive to T. gondii (31.8%) while cats with relatively high seropositivity rates were at 1 or 3.4 years of age (13.14%).

6

Development of Hydrophobic Surgical Forceps using Powder Injection Molding and Surface Treatment

63%

Park D. Y. , Park B. R. , Gal C. W. , Lin D. , Han J. S. , Jeong M.-S. , Bollina R. , Hwang W. , Park S. J.

Archives of Metallurgy and Materials

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2018

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tom Vol. 63, iss. 1

473--480

EN

Hydrophobic surgical forceps of end-effectors for laparoscopic operations or minimally invasive surgery were developed through powder injection molding (PIM) and surface treatment. Processing conditions for mixing, debinding, and sintering were investigated to produce defect-free components. An optimum solid loading was determined by torque rheometry experiments. The optimized processing conditions for debinding and sintering were designed through the measurement of weight loss and shrinkage behavior by thermogravimetric analysis and dilatometry experiments. After producing the surgical forceps based on the optimized processing conditions via PIM, surface treatment was carried out to generate the hydrophobic structure on the surface.

7

Cloning, in silico characterization, subcellular localization, and expression of a heat shock cognate 70 kDa protein/gene (EjHsc70-2) from Eriobotrya japonica

51%

Lin S. , Wu T. , Li M. , Huang X. , Zhang Y. , Han L. , Wu B. , Chen Y. , Lin S. , Lin D. , Wu M. , Wu J.

Acta Physiologiae Plantarum

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2019

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tom 41

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nr 07

EN

Heat shock protein 70 kDa proteins (Hsp70 s) are among the most important molecular chaperone groups and play a significant role in the stress responses and development of plants. In the present study, the full-length cDNA of the heat shock cognate 70 protein 2 gene EjHsc70-2, which encodes a loquat Hsp70 s member, was cloned and characterized, and its expression and subcellular localization were also investigated. The full-length cDNA of EjHsc70-2 consists of an open reading frame (ORF) of 1950 bp, a 5′-UTR of 103 bp, and a 3′-UTR of 62 bp, and the ORF encodes 649 amino acid residues. The structure of the loquat Hsc70-2 protein was analysed using several bioinformatics tools, and the results showed that the protein was, indeed, a member of the Hsp70 s. Phylogenetic tree analysis suggested that the genetic evolution of Hsc70-2 genes conformed well to the morphology based taxonomic classification of seed plants. BLAST and multiple alignment analyses determined that the Hsc70-2 genes and Hsc70-2 proteins were both highly conserved among loquat and other seed plants, suggesting that the functions of EjHsc70-2 might be similar to those of other Hsc70-2 genes. The bioinformatics and experimental subcellular localization analyses both supported that EjHsc70-2 was a cytoplasmic and/or nuclear protein. Quantitative real-time RT-PCR (RT-qPCR) suggested its conserved functions involved in loquat organ development. Moreover, EjHsc70-2 were also inducible, which may contribute to the low-temperature adaptation of loquat fruits in cold storage. These results provide new insights into the characteristics and functions of Hsp70 s in Eriobotrya japonica.