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EN
A validated method for the determination of δ99-1, were the following: limit of detection -0.25; limit of quantification- 0.5, for both analytes; linearity range-0.5-100 for 9THC and THCCOOH in blood, and 0.5-200 for THCCOOH in urine. Internal standardization was carried out using deuterated analogues of the analytes. Intra- and inter-day precision did not exceed 25% for 9THC and 7% for THCCOOH. Accuracy was confirmed using Medidrug reference materials. The method was applied to the determination of 9THC and THCCOOH in 15 blood and 2 urine samples collected from drivers suspected of driving under the influence of cannabis.
PL
Przedstawiono zwalidowaną metodę oznaczania δ99
EN
The aim of the study was to estimate the effectiveness of intestinal scraping technique (1ST) in the detection of Echinococcus multilocularis. The experimental assessment of the limit of detection and comparison with "gold standard" (sedimentation and counting technique - SCT) was also performed. Samples of fox small intestines experimentally enriched with known numbers of E. multilocularis tapeworms, were used. Twenty four samples containing 10, 30, 60, and 90 E. multilocularis tapeworms were prepared. Moreover, in order to compare IST with SCT, 127 intestines of foxes were examined using both methods. The limit of detection was estimated at 30 E. multilocularis tapeworms per sample of the intestine. Moreover, mean number of Echinococcus found by IST were several dozen times lower than the real content of these tapeworms in the samples (on average only 2 to 3.2% of worms were recovered). Among 127 intestinal samples examined with the use of two methods, eight samples (8.2%) were positive by SCT and only two ( 1.6%) when 1ST was used. A relatively high limit of detection estimated experimentally in the first part of the study, as well as, the results obtained in field investigations showed clearly that IST method could significantly decrease the reliability of the results of investigations, especially carried out in regions where a very low prevalence of E. multilocularis occurs or in countries, which want to demonstrate that they are free from this parasite.
PL
Celem badań było określenie wpływu środowiska wodnego na DTS żywic kompozytowych stosowanych do wykonawstwa protez stałych. Badania wykazały, że wszystkie poddane ocenie materiały po 1 tygodniu przechowywania w środowisku wodnym wykazywały istotny wzrost wartości DTS, przy jednoczesnym zróżnicowanym stopniu chłonięcia wody, a po ekspozycji przez 1 miesiąc zarejestrowano istotny spadek wartości DTS, przy równoczesnym zdecydowanym wzroście sorpcji wody.
EN
Four com. dental composites were light cured, stored in distd. water at 37°C for 7 or 30 days and studied for water absorbability and diam. tensile strength. The composites differed in water uptake. Their strength showed a max. when plotted against the time of storage in water.
EN
The aim of the study was to estimate the prevalence of gastrointestinal parasites in raccoons with particular regard to zoonotic parasites. Fifty-five raccoons, hunted or found dead on roads, were examined. The small and large intestines were collected from all raccoons and, additionally, the stomach was collected from 43 animals. The samples were examined with the use of sedimentation and counting technique. The intestines and stomach were examined separately. Samples of raccoon faeces were collected from their environment localised in Słubice district, Lubuskie province (Poland). The samples were collected once a month in 2012. In total, 154 faecal samples were obtained and examined with the use of McMaster flotation technique. The following parasites were detected in the intestinal and stomach contents: tapeworms Mesocestoides sp. (67.3%), Echinostomatidae flukes (34.5%), and nematodes Capillaria sp. (25.5%). Moreover, Acanthocephala were found in the intestines of three raccoons. The highest intensity of infection were observed in case of Mesocestoides sp. Mesocestoides sp. and Echinostomatidae were found statistically more often in the intestines than in the stomach. In the case of these two parasites, there was positive correlation between the intensity of infection in the intestines and the presence of the same parasites in the stomach. Moreover, significantly higher prevalence and intensity of Mesocestoides sp. in males than in females were also observed. Faecal samples contained Baylisascaris procyonis eggs (mean 60 epg). These eggs were found in three samples collected in November and December. Furthermore, in some faecal samples eggs of flukes, Capillaria sp., Mesocystoides sp., and coccidian oocy sts were found. It is one of rare reports concerning Baylisascaris procyonis in Poland confirming the presence of this dangerous parasite in Polish raccoon population.
14
Content available remote Profilometr laserowy do odtwarzania geometrii powierzchni
38%
PL
W artykule przedstawiono opracowany profilometr z laserową głowicą triangulacyjną i mechatronicznym układem pozycjonowania badanych obiektów. Podczas skanowania powierzchni z zastosowaniem techniki triangulacyjnej pojawiają się charakterystyczne zakłócenia związane głównie z występowaniem dużych obszarów cienia, w wyniku zjawiska przesłaniania. W opracowanym profilometrze zastosowano oryginalną metodę ograniczenia zakłóceń, polegającą na wielokrotnym skanowaniu powierzchni obiektu przy wybranych położeniach kątowych.
EN
The paper presents a profilometer equipped with a laser triangulation head and a mechatronic positioning system for tested objects. The triangulation technique produces characteristic disturbances - mainly related to the shadowed areas known as occlusions. In the presented approach the occlusions are eliminated by multiple scanning of the object in several different angular orientations of a scanning line sensor relative to the object in question.
19
Content available remote Szybki skaner optyczny wykorzystujący sensory konfokalne
38%
PL
Do badania kształtów wykorzystywane są różne techniki pomiarowe. Wybór konkretnego typu urządzenia zależy od wielu parametrów, takich jak wielkość i stopień skomplikowania kształtu mierzonego obiektu, jego budowa, żądany czas pomiaru oraz wymagana dokładność. Niniejszy artykuł przedstawia metodykę szybkiego skanowania z wykorzystaniem sensorów konfokalnych, przy czym główny nacisk położono na zminimalizowanie czasu pomiaru przy zachowaniu jego dokładności. Przeprowadzono również prace porównawcze otrzymanych rezultatów z wynikami uzyskanymi za pomocą innych optycznych urządzeń pomiarowych.
EN
There is a large collection of shape characterising measurement systems. Selecting a particular instrument depends on: type of the shape, its configuration and complexity, and required time and accuracy of measurements. The paper demonstrates methodology of fast scanning system equipped with chromatic confocal sensors. A comparative analysis includes measurement results received from other types of profilometers.
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