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Free fatty acids concentration in the Baltic Sea surface waters

100%

Pazdro K.

tom 25

nr 3

25-38

Free fatty acids concentrations and composition in dissolved and particulate fraction of the Baltic Sea surface waters (The Gulf of Gda?sk, the Gda?sk Deep and the Gotland Deep) and the Vistula waters were determined by means of fatty acids coumaryl esters HPLC analysis. Total concentrations of dissolved fatty acids were stated between 0.14 and 13 mg ?dm-3, whereas of particulate fatty acids between 16 and 50 mg ? dm-3. The concentrations depended strongly on the time and location of sampling. Palmitic acid was the dominant one in all inves-tigated samples. It was revealed that the total concentration as well as the percentage composi-tion of individual acids (especially polyunsaturated acids) in surface waters were related to the intensity of biological processes of the investigated area. The autochthonous phytoplankton blooms were the main source of fatty acids in the Baltic Sea surface waters.

Determination of free fatty acids in marine environment by means of High Performance Liquid Chromatography (applying their UV absorbing and fluorescent esters)

63%

Pazdro K. , Falkowski L.

Oceanological Studies

1994

nr 67/10

15-26

High performance liquid chromatography was applied for identification and quantitative determination of fatty acids in marine environment, after theirtransformation into UV absorbing or emmiting derivatives.The following esters have been prepared and their chromatographic properties examined: p-nitrobenzyl, 4-methyl-7-metaxocoumaryl and 9-methyl-antranyl.Very low detection limit and efficient separation have been revealed.Reversal phase HPLC has been used for separation and identification of individual fatty acids in marine organisms and selected components of the marine environment, whereas their total concentration has been determined using normal phase chromatography.

The use of the Vibrio harveyi luminescence mutagenicity assay as a rapid test for preliminary assessment of mutagenic pollution of marine sediments

51%

Podgorska B. , Pazdro K. , Wegrzyn G.

Journal of Applied Genetics

2007

tom 48

nr 4

409-412

Mutagenic pollution of the natural environment is currently one of the most serious environmental problems. It includes the pollution of marine sediments. Therefore, rapid detection of the presence of mutagens is an important issue. Recently, we have developed a novel microbiological assay for rapid assessment of mutagenicity of samples from the natural environment. This assay is based on bioluminescence of a mutant Vibrio harveyi strain, and was shown to be useful in testing samples of marine water and plant tissues. Here we demonstrate the usefulness of this assay in preliminary assessment of mutagenic pollution of marine sediments. Mutagenicity of environmental samples taken from the Baltic Sea, is documented and compared here with a commercially available standard sediment sample (IAEA 383), which contains known amounts of mutagenic compounds. The whole procedure, from obtaining a sample in the laboratory to getting final results, is very short (less than 4 h).