The effect of cryopreservation on seed germination and seed vigour, and seeds with different moisture regimes have been studied to find out if they are a good candidate for LN by storing seeds in liquid nitrogen (196 °C) for 24 and 72 hours from three rice seed cultivars, namely Lisem, Manen, and Mapok. For all the three cultivars studied, cryopreservation did not decrease germination percentages or seed vigour and it also appears that seed moisture content ranging from 10% to 18% is not a determining factor for the survival of seeds up to 72 hour storage of the seed in LN.
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