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1 Open Chemistry

1 2010

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Qualitative and quantitative analysis of gallic acid in Alchemilla vulgaris, Allium ursinum, Acorus calamus and Solidago virga-aurea by chip-electrospray ionization mass spectrometry and high performance liquid chromatography

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Condrat D. , Mosoarca C. , Zamfir A. , Crişan F. , Szabo M. , Lupea A.

Open Chemistry

2010

tom 8

nr 3

530-535

This study presents the results obtained from qualitative and quantitative analysis of gallic acid from hydro-alcoholic extracts (methanol, ethanol) of plants from Plantae regnum. Plant qualitative analysis was performed using a novel mass spectrometric (MS) method based on fully automated chip-nanoelectrospray ionization (nanoESI) high capacity ion trap (HCT) while quantitative analysis was carried out by high performance liquid chromatography (HPLC). These methods were applied to Alchemilla vulgaris - common lady’s-mantle (aerial part), Allium ursinum - bear’s garlic (leaves), Acorus calamus - common sweet flag (roots), Solidago virga-aurea - goldenrod (aerial part). Obtained results indicated that methanol extracts (96%, 80%) have a gallic acid content ranging between 0.0011–0.0576 mg mL−1 extract while the ethanol extracts (96%, 60%) exhibit a gallic acid concentration that varies between 0.0010–0.0182 mg mL−1 extract. [...]