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2 Oceanologia

2 1998

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Stress proteins induced by cadmium in the abdominal muscle of the shrimp Crangon crangon [commun.]

100%

Napierska D. , Radłowska M.

tom No. 40 (2)

157-162

Shrimps were exposed to various concentrations of CdCl2 under laboratory conditions for 96 h. Abdominal muscles were isolated from exposed and control animals. The induction of stress proteins (heat shock proteins of the Hsp70 family and metallothionein) was detected following polyacrylamide gel electrophoresis in the presence of SDS (SDS-PAGE) and specific staining (Western blotting method in the case of the Hsp70 or Coomassie blue and silver staining in the case of metallothionein). The short-term cadmium poisoning in the shrimp Crangon crangon resulted in the induction, in a concentration-dependent manner, of metallothionein and a new protein with an approximate molecular weight of 70 kDa in abdominal muscle. This protein was immunologically cross-reactive with the 70 kDa heat shock protein of the mouse.

Induction of stress proteins in the presence of cadmium in the Baltic blue mussel Mytilus trossulus [commun.]

100%

Radłowska M. , Pempkowiak J.

tom No. 40 (2)

153-156

The exposure of organisms to environmental stressors affects the expression levels of certain 'stress proteins' that play an important role in protein homeostasis and stress tolerance. Examining the protein profiles by SDS-PAGE and Western blotting analysis, mussels Mytilus trossulus were exposed to cadmium, which induced a number of Hsp70 proteins in accordance with the metal concentrations. In immunodetection two commercial monoclonal antibodies were used to monitor this response in gill tissue. It follows that Hsp70, which is typically induced by moderate heat-shock treatment, is in most cases also induced in the presence of cadmium.