Wyniki wyszukiwania - Biblioteka Nauki

1

Występowanie zakażeń nowymi gatunkami parwowirusów świń

100%

Podgorska K.

Magazyn Weterynaryjny

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2012

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tom 21

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nr 06 Choroby Świń - Monografia

2

Immunologiczne aspekty PMWS

100%

Podgorska K.

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2008

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tom 03

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nr 1

3

Kierunki badań nad zakażeniami cirkowirusem świń typu 2

100%

Podgorska K.

Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii

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2011

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tom [06]

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nr Monografia. Wyd. Spec.

4

Porcine circovirus type 2 viremia and seroconversion in pigs from a farm affected by postweaning multisystemic wasting syndrome

63%

Podgorska K. , Stadejek T.

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tom 14

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nr 4

EN

The aim of the study was to assess the usefulness of real-time PCR and serological methods as indicators of postweaning multisystemic wasting syndrome (PMWS) occurrence. Significantly higher level of porcine circovirus type 2 (PCV2) viral load in serum and significantly lower titre of specific antibodies in PMWS-affected pigs indicated that combination of quantitative PCR and serological methods may support diagnosis of PMWS.

5

Zakażenia cirkowirusowe świń - aktualna klasyfikacja oraz kryteria diagnostyczne

63%

Szczotka-Bochniarz A. , Podgorska K.

Medycyna Weterynaryjna

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2015

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tom 71

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nr 02

EN

Porcine circovirus type 2 (PCV2) belongs to the Circoviridae family, comprising the smallest viral pathogens. PCV2 is involved in the etiology of several diseases of swine called “porcine circovirus associated disease” (PCVD). The most important disease in this group is postweaning multisystemic wasting syndrome (PMWS). The role of PCV2 in the etiology of PCVD and the ambiguous interpretation of laboratory results have for years been subjects of controversy. Several, interchangeably used, names have been created for clinical syndromes related to PCV2, and unclear diagnostic criteria hampered the identification of diseases. Therefore, in 2012, new terminology and standardized diagnostic rules were proposed for clinical syndromes related to PCV2. The diagnosis of PCV2 systemic disease and of other PCV2-related diseases is based on the detection of the virus associated with characteristic histopathological lesions (by in situ hybridization or immunohistochemistry). Currently, 4 vaccines for PCV2 immunoprophylaxis are commercially available. The dominant genotype is PCV2b, and its prevalence has also been confirmed in Poland. However, all commercially available vaccines are based on the PCV2a genotype. The latest results, published in 2013, indicate that experimental vaccines based on the PCV2b genotype are more effective and offer better protection against infections with PCV2b alone and those involving both PCV2b and PCV2a compared to commercially available vaccines.

6

Przyczyny ujawniania się skutków zakażenia świń PCV2

63%

Podgorska K. , Pejsak Z.

Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii

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2010

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tom 05

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nr 2

7

Nowe parwowirusy świń

63%

Podgorska K. , Szczotka-Bochniarz A.

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tom 70

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nr 04

EN

During the last decade a variety of previously unknown porcine parvoviruses (PPV) were identified. New species were classified as PPV2, PPV3, PPV4, bocaviruses or hokoviruses. Preliminary investigations indicate that some of the newly emerging parvoviruses are relatively widespread in the Polish pig population. These findings suggest that the evolution and diversity of parvoviruses is much more complex than previously assumed. Current scientific efforts focus on issues such as the prevalence, pathogenic potential, genetic and antigenic variability, as well as the further evolution of parvoviruses. The aim of the present study was to summarize current knowledge regarding the newly described parvovirus species. First atypical strains of parvoviruses were detected in Asia and classified as PPV2. Latest studies have showed that PPV2 is present in swine in Hungary. In 2008, Lau et al. described animal parvoviruses similar to the human parvovirus PARV4. After the place of their first isolation (Hong Kong), the names “porcine hokovirus” (PHoV) and “bovine hokovirus” (BHoV) were proposed. In 2010, Cheung et al. suggested including PHoV in the Parvovirus genus and changing its name to “PPV3,” but this classification is still unofficial. The presence of PPV3 genotype was confirmed in pigs in Hungary, Great Britain, and Romania, as well as in the population of wild boars in Germany. Another species, PPV4, was found in 2005 in North Carolina (USA). PPV4 has also been detected in China, in both healthy and diseased pigs. In Hungary, PPV4 infection has been confirmed in 13 out of 57 herds analyzed, which suggests a large prevalence of this virus in the swine population. The presence of bocaviruses has been detected in pigs in Sweden, China, and Hungary, as well as in wild boars in Romania. Preliminary results suggest that in the swine population in Poland, not only classical PPV1, but also PPV2, PPV3, and PPV4 are present.

8

Aktualne dane na temat zakażeń cirkowirusowych świń

63%

Szczotka-Bochniarz A. , Podgorska K.

Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii

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2013

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tom [08]

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nr Monografia Wyd. Spec.

9

Charakterystyka nowych gatunków parwowirusów świń

63%

Podgorska K. , Szczotka-Bochniarz A.

Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii

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2013

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tom [08]

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nr Monografia Wyd. Spec.

10

Effect of porcine circovirus type 2 vaccination of sows on selected production parameters

51%

Pejsak Z. , Truszczynski M. , Porowski M. , Podgorska K.

Bulletin of the Veterinary Institute in Puławy

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2009

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tom 53

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nr 2

159-164

EN

An aim of the study was to evaluate the efficacy of Circovac vaccine in two Polish pig herds affected by post-weaning multisystemic wasting syndrome (PMWS) and reproductive failure. The effectiveness of vaccination was assessed indirectly by analysis of production performance of the examined herds. It was found that Circovac is extremely efficient in reducing losses attributed to PMWS and controlling the disease. Analysis of production parameters after vaccination showed significant improvement in the nursery and finishing sectors. Post-weaning mortality dropped nearly five times in herd P and 2.5 times in herd D. Mortality among fatteners decreased by half in both herds. Additionally, significant improvement was observed concerning average daily weight gains of weaners and fatteners. Despite the considerable benefits of vaccination in nursery and finishing pigs, a tendency to increase reproductive parameters was observed. The most striking differences concerned returns to oestrus and abortion rates, which reached more favourable levels even compared to the period before the PMWS episode. This observation suggests that subclinical infections with PCV2 may have negative effects on the reproduction.

11

Pictorial review of COVID-19 in the Lublin Region – Imaging disease progression with CXR and CT

51%

Kurys-Denis E. , Podgorska K. , Prystupa A.

Journal of Pre-Clinical and Clinical Research

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2021

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tom 15

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nr 1

12

Profile serokonwersji dla zespołu rozrodczo-oddechowego świń w wybranych stadach świń w Polsce

51%

Czyzewska E. , Podgorska K. , Dors A. , Pejsak Z.

Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii

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2012

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tom 07

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nr 4

13

Influence of long-term vaccination of a breeding herd of pigs against PCV2 on reproductive parameters

51%

Pejsak Z. , Kusior G. , Pomorska-Mol M. , Podgorska K.

Polish Journal of Veterinary Sciences

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2012

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tom 15

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nr 1

EN

The objective of the study was to evaluate an efficacy of sows vaccination protocols in the herd with serious problems affecting efficacy of reproduction. The study was performed in a large pig herd with about 1200 sows. Before vaccination against PCV2, farrowing rate in this farm was about 65%. Sows, boar and replacement gilts were immunized using Circovac vaccine (Merial, France) according to producer’s recommendations. Parameters of production were analyzed since 2007 until 2010 in selected batches of sows inseminated at the same weeks of the year (17th, 18th, 19th and 20th) to eliminate seasonal variability. In total, 940 sows were subjected to the study. No significant changes in management during these years were introduced. The applied protocol of sow herd long-term vaccination proved to be very efficient. All measured production parameters: reproduction rate, number of piglets born alive, birth weight of piglets and number of piglets weaned per a litter improved after implementation of immunization program. Moreover, further improvement was observed with vaccination in the following reproduction cycles. The most spectacular effect of vaccination regarded average farrowing rate that increased from 64.76% in control group to 86.93% after basic vaccination. Two years after implementation of vaccination program this parameter reached 93.6%. Number of piglets weaned per sow per a litter improved from 10.31 to 11.74 after one year of vaccination and remained relatively stable through the following year. Simultaneously, the percentage of newborn piglets with birth weight < 1 kg decreased significantly (p < 0.05). To summarize, vaccination against PCV2 influenced positively the insemination rate, number of piglets born alive and weaned per litter as well as birth body weight and percentage of piglets weighing < 1 kg.

14

Wybrane dane prezentowane podczas 25. Kongresu IPVS. Część III. Cirkowirusy i koronawirusy świń

51%

Pomorska-Mol M. , Podgorska K. , Urbaniak K.

Życie Weterynaryjne

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2019

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tom 94

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nr 05

15

Szczepionka przeciwko afrykańskiemu pomorowi świń. Obecny stan badań, trudności i perspektywy

45%

Walczak M. , Juszkiewicz M. , Wozniakowski G. , Szymankiewicz K. , Podgorska K.

Życie Weterynaryjne

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2023

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tom 98

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nr 11

16

Pierwszy przypadek poodsadzeniowego wielonarzadowego zespolu wyniszczajacego swin w Polsce

38%

Stadejek T. , Podgorska K. , Kolodziejczyk P. , Bogusz R. , Kozaczynski W. , Pejsak Z.

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tom 62

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nr 03

297-301

EN

The present study describes first clinically detected and confirmed by laboratory methods case of PMWS in Poland. The investigation was carried out in a large farrow-to-finish farm producing 40 000 fatteners per year. In the spring of 2000, growth retardation, wasting and respiratory disorders affected 20% of weaners and 30% of fatteners. Nineteen weaners and 11 fatteners with different degrees of wasting, respiratory disorders, skin paleness and hemorrhagic skin lesions (fatteners only) were necropsied. Post-mortem examination revealed the presence of inflammatory signs in lymphoid tissues, enlarged lymph nodes, non-collapsed lungs, enlarged livers, enlarged and pale kidneys with generalized cortical petechiae. Sections of lymph nodes were submitted for histopathological examination and in situ hybridization. Moreover, virus isolation in PK15 cell line was attempted. Histopathological analysis of lymph nodes revealed different degrees of lymphatic depletion and histiocitic infiltrations, giant cells and intracytoplasmic inclusion bodies. In situ hybridization with the use of a specific for PCV2 digoxigenin labelled DNA probe confirmed the abundant presence of PCV2 DNA within lesions in lymph nodes. A peroxidase assay indicated PCV2 replication in the inoculated cell line. Gross and histopathological lesions and the presence of PCV2 antigens in lymphoid tissues confirmed the presence of PMWS in a Polish swine herd. Clinical signs and laboratory examinations indicated that PDNS and PMWS coexisted in the herd. This suggests that PCV2 could be an important factor in an aetiology of PDNS.

17

Wystepowanie poodsadzeniowego wielonarzadowego zespolu wyniszczajacego w krajowej populacji swin

38%

Podgorska K. , Stadejek T. , Szczotka A. , Kozaczynski W. , Porowski M. , Pejsak Z.

Medycyna Weterynaryjna

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2009

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tom 65

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nr 05

330-333

EN

The aim of the study was to assess the occurrence of the postweaning wasting syndrome (PMWS) in Polish pig herds. Thirty-two herds were selected from 195 visited pig production sites based on the appearance of clinical lesions. The population of breeding herds ranged from 25 to 2000 sows and the percentage of losses varied from 2 to 20%. In each farm 3 pigs affected by wasting were autopsied and lymph nodes were collected. Sections of lymph nodes were examined for the presence of histopathological lesions and tested with in situ hybridization to detect the DNA of porcine circovirus type 2 (PCV2), an etiological agent of PMWS. The results were considered as positive when characteristic histopathological changes were detected along with an abundant amount of viral DNA. PMWS was recognized in 26 pigs (27.9%) from 16 farms (50%). In two of those farms PMWS was diagnosed as sporadic, based on the analysis of production parameters and low rate of losses. In 12 herds (37.5%) no pig had DNA of PCV2 in lymph nodes, while in the remaining 4 herds (12.5%) low amounts of DNA were detected in lymph nodes from at least one pig. PMWS was confirmed in all tested farms with breeding herds of more than 1000 sows, while none of the small farms with breeding herds of less than 100 sows were affected. In two other groups of farms counting 100-499 and 500-1000 sows the percentage of affected herds was respectively 33.3% and 57.1%. The established rate of PMWS occurrence was similar to that claimed by most other European countries. Results obtained in this study suggest that the incidence of PMWS outbreaks increases with the size of the herd. A higher number of properly selected samples markedly enhances the probability of disease detection.

18

Application of real-time PCR for detection of Lawsonia intracellularis and Brachyspira hyodysenteriage in fecal samples from pigs

38%

Zmudzki J. , Szczotka A. , Podgorska K. , Nowak A. , Grzesiak A. , Dors A. , Pejsak Z.

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nr 2

EN

The aim of the study was to develop and validate real-time PCR method for the quantification of Lawsonia intracellularis and Brachyspira hyodysenteriae in porcine feces. Before the optimization process was performed two different extraction methods were compared to select the more efficient one. Based on the results achieved at this stage the boiling procedure was rejected and a commercially available silica-membrane based method was chosen for further analysis. The primers and the Taqman probe for B. hyodysenteriae and L. intracellularis were based on the sequence of NADH oxidase gene and 16S rDNA gene, respectively. The detection limit of the real-time PCR for suspension of feces inoculated with B. hyodysenteriae and L. intracellularis was determined to be 1.5x103 CFU/ml and 6.5x101 CFU/ml, respectively. The results of this study demonstrate that our real-time PCR is able to detect low number of B. hyodysenteriae and L. intracellularis cells which is satisfying in routine diagnosis of swine dysentery and proliferative enteropathy. Therefore, it is possible to identify both subclinically infected pigs and those representing an acute form of mentioned diseases. In summary, the quantitative real-time PCR is useful for routine diagnosis of L. intracellularis and B. hyodysenteriae. Compared to conventional PCR, the new validated quantification method based on real-time PCR is fast and with reduced risk of laboratory contamination. The novel technique is specific and even more sensitive than the previously used one. Furthermore, the new real-time PCR enables quick detection and quantification of both pathogens in fecal samples, which helps to estimate the health status of a pig herd.

19

Proinflammatory cytokine changes in bronchoalveolar lavage fluid cells isolated from pigs infected solely with porcine reproductive and respiratory syndrome virus or co-infected with swine influenza virus

32%

Czyzewska-Dors E. , Pomorska-Mol M. , Dors A. , Pluta A. , Podgorska K. , Kwit K. , Stasiak E. , Lukomska A.

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2019

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tom 63

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nr 4