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Determination of lysozyme enthalpic pairwise interaction parameters using a titration calorimeter at 288.15, 298.15 and 308.15 K

100%

Wszelaka-Rylik M.

Bulletin of the Polish Academy of Sciences. Chemistry

2003

tom Vol. 51, No. 2

135-141

The titration Omega MicroCal microcalorimeter (ITC) was used to obtain the enthalpy changes accompanying dilution of buffer solutions of lysozyme. Titration calorimetry affords a convenient method for the estimation of enthalpic pairwise solute-solute interaction parameters (hxx}- In this paper the results of determination of the enthalpy of dilution and then evaluated pair interaction coefficients (hxx) of lysozyme in a buffer containing 0.1 M sodium acetate, pH 4.25 at temperatures 288.15, 298.15, and 308.15 K are presented.

A diadenosine 5',5''-P1P4 tetraphosphate [Ap4A] hydrolase from Arabidopsis thaliana that is activated preferentially by Mn2plus ions

44%

Szurmak B. , Wyslouch-Cieszynska A. , Wszelaka-Rylik M. , Bal W. , Dobrzanska M.

Acta Biochimica Polonica

2008

tom 55

nr 1

Asymmetrical diadenosine 5',5''-P 1P 4 tetraphosphate (Ap4A) hydrolases are key enzymes controlling the in vivo concentration of Ap4A - an important signaling molecule involved in regulation of DNA replication and repair, signaling in stress response and apoptosis. Sequence homologies indicate that the genome of the model plant Arabidopsis thaliana contains at least three open reading frames encoding presumptive Ap4A hydrolases: At1g30110, At3g10620, and At5g06340. In this work we present efficient overexpression and detailed biochemical characteristics of the AtNUDX25 protein encoded by the At1g30110 gene. Aided by the determination of the binding constants of Mn(Ap4A) and Mg(Ap4A) complexes using isothermal titration calorimetry (ITC) we show that AtNUDX25 preferentially hydrolyzes Ap4A in the form of a Mn 2+ complex.

Wind rose and modelling of the fate of polynuclear aromatic hydrocarbons in the Central Poland

38%

Gierycz P. , Malanowski S. K. , Wszelaka-Rylik M. , Kacperski W. T. , Kowalczyk D. , Świetlik R.

Environment Protection Engineering

2016

tom Vol. 42, nr 4

17--35

The wind rose analysis has been performed for the town of Radom in Central Poland. It was found that West and South-West wind directions significantly dominate in the wind rose, causing PAHs influx from coal power stations. The analysis of the PAHs accumulation in water, soil and bottom sediments based on the fugacity and partition coefficients model has been performed for Domaniów Lake. The results have been compared with the experimental PAHs concentrations in the lake, in- and out-flowing waters as well as in the dust over the lake.