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1 Biotechnologia

1 2001

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Agronomic performance of wheat doubled haploids produced through wheat x maize crosses

100%

Guzy-Wrobelska J. , Szarejko I. , Nawrot M. , Madajewska M.

Biotechnologia

2001

nr 2

72-79

Wheat x maize crosses are used as a method of wheat doubled haploid (DH) production, alternative to anther culture. The study was conducted to compare the agronomic performance of DH lines produced through wide crosses with lines obtained by the single seed descent (SSD) method from the same plant material. F1 progeny of spring wheat varieties: Eta x Sigma (both Polish) and Eta x Darkhan 15 (Mongolian) were used for DH and SSD production. Doubled haploids (DH3, DH4) and SSD (F5) lines from both genotypes were evaluated for plant height, spike length, tillering, grain weight and number per plant and 1000 grain weight in a randomised, three replicated experiment. Mean performance, coefficient of variation and frequency distribution of DH and SSD lines were similar for most of the analysed traits. The comparison of the best 10% of DH and SSD lines from both crosses (selected on the basis of grain weight per plant) confirmed their similar performance. For the majority of the analysed characters, the best DH lines derived from both DH populations did not differ from better parents and check varieties. Ten percent of Eta x Sigma DH lines performed significantly better for 1000 grain weight than both parental genotypes, heterotic F1 and check varieties. The results indicate that maize pollination system is an efficient method of producing high yielding homozygous lines of wheat and it may be recommended for a wide use in wheat breeding programmes.