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3 2014

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Precision-cut luteal slices: A promising approach for studying luteal function in pigs

100%

Przygrodzka E. , Lopinska M. , Ziecik A. J.

tom 14

nr 3

Effect of vaginal administration of prostaglandin E2 and/or 17Betta-estradiol on luteal function and histological characteristics of the cervix in cyclic pigs

100%

Przygrodzka E. , Andronowska A. , Janowski T. , Ziecik A. J.

nr 1

The overall objective of this study was to examine the effect of vaginal administration of prostaglandin E₂ (PGE₂) and/or17β-estradiol (E₂) on luteal function maintenance and histological properties of the porcine cervix. For this purpose, crossbred gilts were divided into three groups (n=5 per group) supplied on days 11-16 of the estrous cycle with suppositories containing: (1) placebo (Group I, Control); (2) 0.4 mg of E₂ (Group II); (3) 0.4 mg of E₂ and 2 mg of PGE₂ (Group III). Blood samples were collected on days 11-19 of the estrous cycle to determine the concentration of progesterone (P₄). Additionally, to examine local effects of the hormones applied, segments from the uterine and vaginal parts of the cervix and from the ovaries were collected post-mortem. Prolonged luteal function and extended synthesis of P₄ were observed in 2 of 5 gilts receiving PGE₂ and E₂ simultaneously (Group III). Then, these gilts were subdivided into Group IIIA (n=2; presence of corpora lutea on the ovaries) and Group IIIB (n=3; lack of corpora lutea). Increased levels of plasma P₄ were observed in Group IIIA on days 15-19 compared to Group IIIB and on days 16-19 compared to Group I and Group II (P<0.05; P<0.01; P<0.001, respectively). In the cervix of gilts in Groups II and III, enlarged blood vessels in the lamina propria of both parts of the cervix were observed. Furthermore, in Group II the epithelium of the uterine part of the cervix was thicker (P<0.001). Our study confirmed the proposed luteotrophic/antiluteolytic actions of E₂ and PGE₂ applied intravaginally. These results are significant considering that very low doses of E₂ were used when compared to previous attempts. Despite the inadequate response to treatments in some of the gilts, the local effects of these hormones on the histological properties of the porcine cervix suggest that further improvements in the vaginal administration route might help to elaborate new methods for enhancing the luteal function in the pig.

Effect of hCG and intravaginal application of estradiol and prostaglandin E2 on pregnancy rate and litter size in gilts and sows

88%

Ziencik A. J. , Lopinska M. , Przygrodzka E. , Wasielak M. , Kempa W.

nr 1

Three separate experiments were performed on fifty pregnant gilts (Experiment 1 and 2) and forty primiparous sows (Experiment 3) to examine the effect of human chorionic gonadotropin (hCG) administration on day 12 (Experiments 1 and 2) or 20 (Experiments 2 and 3) of pregnancy and intravaginal application of estradiol alone or estradiol + prostaglandin E2 (PGE2) on days 17-23 (Experiment 3) of pregnancy on pregnancy rate and litter size and weight at birth and weaning. None of the treatments affected the pregnancy rate which ranged between 90 and 80% in control and treated groups. In Experiment 1 no differences in the number of piglets born (13.2±1.1 or 13.0±0.8) and weaned between control and hCG group were noticed. In Experiment 2 hCG treatment increased the total number of piglets born. For treatment on day 12 this number amounted to 15.1±1.3, for day 20 – 16.4±1.4, while for control gilts 13.0±1.5. The similar trend occurred in the number of piglets born alive. The remaining indicators of piglets survival did not vary between groups. Though there were no significant differences in the number of total piglets born between groups of sows in Experiment 3, a trend to the increased number of piglets weaned was found in groups with intravaginal treatment of estradiol and PGE2 (P= 0.10 ) or hCG administered on day 20 of pregnancy. The study revealed, for the first time, that hCG or intravaginal estradiol and PGE2 administration during early pregnancy did not affect the pregnancy rate in gilts and sows and can be beneficial for litter size.