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EN
Lawsonia intracellularis, a Gram-negative, curved rod was identified as the causative agent of porcine proliferative enteritis (PPE) in the 1990's. The bacterium is an obligate intracellular parasite, and thus cannot be multiplied in-vitro on standard media. Nowadays, two types of the disease have been distinguished: acute and chronic. Pigs with PPE usually demonstrate clinical signs two weeks after a period of stress caused by transportation, heat, overcrowding or weaning piglets from sows. The infected animals suffer from diarrhea and indicate reduced growth rate and weight gain. In some cases the disease leads to death. PPE is difficult to diagnose as both its clinical signs and the pathological changes it causes are unspecific. The pathogen is identified in laboratory conditions by the use of PCR, and IFAT is applied to track specific antibodies. Tiamulin and tylozyne are the most popular drugs used to prevent PPE.
EN
Principal aim of this study was to examine fecal samples from pigs suffering from diarrhea for the presence of Lawsonia intracellularis, Brachyspira hyodysenteriae and Brachyspira pilosicoli. The molecular techniques such as PCR and nested PCR were employed to detect the presence of p78 fragment of genomic DNA specific for Lawsonia intracellularis as well as fragment of tlyA gene specific for Brachyspira hyodysenteriae and 16S rDNA gene of Brachyspira pilosicoli. We assumed that about 25% of pigs were infected with Lawsonia intracellularis, about 10% with Brachyspira hyodysenteriae and only 0,8% with Brachyspira pilosicoli. In about 3% mixed infection with L. intracellularis and B. hyodysenteriae was observed. Results were comparable in herds that differed in quantity, breeding technology, hygienic standards and preventive treatment with different chemotherapeutics.
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