Based on a three-year field experiment under controlled condition in Ji’nan, China, the effects of peanut growth on the variation in the abundance and community structure of ammonia oxidizing bacteria (AOB) and archaea (AOA) before and after peanut growth were investigated through quantitative PCR and cluster analysis of terminal-restriction fragment length polymorphism. Our results show that the community composition of AOA and AOB was greatly affected by the peanut growth leading to the decreased abundance of AOA and increased abundance of AOB. Furthermore, AOA and AOB community structures varied before and after peanut growth. Phylogenetic analysis indicated that all AOA and AOB community sequences were clustered into the uncultured group. Altogether, the results suggested that the abundance of AOA and AOB in soil and their community compositions can be greatly affected by the peanut growth.
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To understand the high temperature effects on the mechanical and failure behaviors of rock, uniaxial compression tests were carried out on granite specimens containing three pre-existing holes using a rock testing system. Based on the experimental results, the influences of testing temperature on the physical and mechanical parameters of granite were analyzed in detail. An obvious color change of tested granite occurs from gray at room temperature to reddish after 450 °C and to red-brown after 900 °C high temperature treatment. The granite volume increases, mass decreases and density decreases with increasing testing temperature. As the temperature increases, the peak strength first increases and then decreases, while the elastic modulus decreases. However, the peak strain changes slightly before 450 °C, increases dramatically up 450 °C. As the bridge angle increases, the mechanical parameters of granite specimens first decrease and then increase. And then, the crack initiation, propagation and coalescence behavior of granite specimens after high temperature exposure was investigated using an acoustic emission (AE) and photography monitoring technique. The cracking process shows that the propagation of crack from the surface of holes leads to the coalescence between adjacent holes. A large AE count and a stress drop are observed during the crack initiation and propagation. The failure modes can be generally classified into three categories: splitting mode, shear mode and mixed mode and they are closely related to heat treatment temperature and bridge angle. Finally, the mechanism causing the differences in the mechanical parameters observed with increasing temperature was discussed based on the SEM observations.
Hydrogen sulfide (H₂S) has the ability to strengthen plant stress tolerance; however, the effects of H₂S on wheat seedlings under salt stress and the underlying molecular mechanism are still unclear. This study examined the effects of exogenous NaHS as H₂S donor on photosynthesis, antioxidant system, and the expression profile of genes related to antioxidant defense responses, the salt overly sensitive (SOS) and mitogen-activated protein kinase (MAPK) pathways in wheat seedlings treated with NaCl stress. H₂S application improved photosynthesis, and decreased H₂O₂ and malondialdehyde (MDA) contents in wheat seedling leaves under NaCl stress. In addition, antioxidant enzyme activity and the content of ascorbic acid and reduced glutathione increased with H₂S application. Moreover, H₂S pretreatment up-regulated expression levels of genes related to antioxidant system, SOS pathway and MAPK pathway as well as the transcription factor dehydration-responsive element binding gene. Overall, these findings suggest that H₂S alleviates salt stress in wheat seedlings not only by strengthening antioxidant defense systems, but by coordinating signal transduction pathways related to the stress response at a transcriptional level.
This study comprehensively investigated the concentrations, distribution patterns, sources, and health risks of 16 polycyclic aromatic hydrocarbons (PAHs) found in soils around two thermal power plants in Shandong, China. The total concentrations of the 16 total PAHs (Σ₁₆PAH) in the Longguang (LG) and Xinyuan (XY) thermal power plants were 1,031.78-2,744.06 and 1,383.8-2,924.8 μg/kg, respectively, with mean values of 1,925.2 and 2,303.1 μg/kg, respectively. Seven carcinogenic PAHs accounted for 45.5% and 55.7% of Σ₁₆PAHs in soils around LG and XY thermal power plants, respectively. A similar composition pattern of PAHs was observed in soils around the two power plants. The contributions of four-ring PAHs, namely, fluoranthene (FLA), pyrene (PYR), benz[a]anthracene (BaA), and chrysene (CHR), were high; thus, the four-ring PAHs dominated in soils around the two power plants. With the increase of distance, most individual PAH concentrations decreased, and ∑₁₆PAHs concentrations decreased significantly from 200 m to 500 m. However, the contributions of two- to three-ring PAHs increased with distance, whereas the contributions of four-ring PAHs decreased. Four PAH sources in soils around the two thermal power plants were identified by positive matrix factorization. Coal combustion was the main source of soil PAHs. Health-risk assessment based on toxic equivalency factors of benzo[a]pyrene indicated no risk of PAH contamination in soils around LG power plant and a low risk in those around XY power plant. This study highlights the distribution and sources of PAHs in soil around thermal power plants.