Wyniki wyszukiwania - Biblioteka Nauki

1

Doksorubicyna - nowe oblicze starego leku

100%

Lipinska N. , Kopczynski P. , Holysz H. , Rubis B.

Postępy Biologii Komórki

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2013

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tom 40

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nr 4

2

Clinical usefulness of assessing VEGF and soluble receptors sVEGFR-1 and sVEGFR-2 in women with breast cancer

88%

Thielemann A. , Baszczuk A. , Kopczynski Z. , Kopczynski P. , Grodecka-Gazdecka S.

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2013

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tom 20

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nr 2

EN

Introduction. The biological activity of VEGF depends on the presence of its specific receptors on the endothelial surface: VEGFR-1, VEGFR-2, and on their soluble forms sVEGFR-1 and sVEGFR-2. The binding of the membrane-bound receptors with VEGF affects the permeability, proliferation and migration of vascular endothelial cells. This creates the necessary conditions for the vascularisation of solid tumours and for the spread of remote metastases. The sVEGFR-1 and sVEGFR-2 receptors are believed to be natural inhibitors of VEGF. Objective. To determine the clinical usefulness of VEGF and the sVEGFR-1 and sVEGFR-2 receptors level assay in women with primary breast cancer. The assessment also took into account: patient’s age, stage of the disease, histological grade, status of the axillary lymph nodes and size of the primary tumour. Material and methods. The concentrations of VEGF, sVEGFR-1 and sVEGFR-2 were ascertained in 103 women with primary breast cancer. The concentrations of VEGF in the plasma, and those of the soluble receptors sVEGFR-1 and sVEGFR-2 in the serum, were assessed by ELISA, R&D Systems. Results. The study found significantly raised concentrations of VEGF, sVEGFR-1 and sVEGFR-2 in the serum of women with breast cancer, relative to the values obtained from the control group. It was found that with increasing clinical stages of the disease, the levels of VEGF and concentrations of sVEGFR-1 and sVEGFR-2 also increased. Similar findings were noted when assessing the degree of the histological grade of the tumours. Significantly higher values of VEGF protein and the assessed receptors were obtained from women with metastases to the axillary lymph nodes. A positive relationship, though without statistical significance, was noted between the concentration of sVEGFR-2 and the size of the tumour. Conclusions. The high concentrations of the VEGF cytokine and the sVEGFR-1 and sVEGFR-2 receptors in women with breast cancer are responsible for giving rise to the processes of tumour angiogenesis. The concentrations of the VEGF protein and the soluble forms of the receptors sVEGFR-1 and sVEGFR-2 in the serum of breast cancer patients showed positive correlations with the clinical stage of the disease. These results point to the usefulness of VEGF assessment and its soluble receptors in the clinical evaluation of patients with breast cancer.

3

Udział paraoksonazy 1 (EC 3.1.8.1) w rozwoju miażdżycy

88%

Banaszewska A. , Baszczuk A. , Kopczynski Z. , Thielemann A. , Kopczynski P.

Postępy Biologii Komórki

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2014

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tom 41

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nr 3

4

Plasma ghrelin and interleukin-6 levels correlate with body mass index and arterial blood pressure in males with essential hypertension

75%

Gibas-Dorna M. , Nowak D. , Piatek D. , Pupek-Musialik D. , Krauss H. , Kopczynski P.

Journal of Physiology and Pharmacology

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2015

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tom 66

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nr 3

5

Activity of antioxidant enzymes and concentration of Mn, Cu, Zn and protein in porcine follicular fluid

75%

Lasota B. , Ogonski T. , Blaszczyk P. , Seremak B. , Ligocki M. , Telesinski A. , Kopczynski P.

Acta Scientiarum Polonorum. Zootechnica

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2011

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tom 10

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nr 1

EN

Oxidative metabolism is essential for the gamete and the embryo energy production and is unavoidably associated with generation of reactive oxygen species (ROS). Enzymatic antioxidant defenses are present in the mammalian oocytes, embryos and follicular fluid (FF). An addition of porcine FF to maturation media have positive effects on the IVM and IVF results. The aim of this study was to study the CAT, SOD and GSH-Px activity, as well as Cu, Mn, and Zn concentration in porcine FF collected from the left and the right ovary. The ovaries were collected from 77 gilts at age 8 months. All the analyzed samples of FF revealed active enzymes (24.2·10–3 U · l–1, 2.65·10–3 U · l–1, and 525 U · l–1 for CAT, GSH-Px, and SOD, respectively) and contained Zn, Cu and Mn (13.8·103mol · l–1, 33.3·103 mol · l–1, and 133·10–9 mol · l–1, respectively). In the pFF collected from the left ovary, SOD and GSH-Px activity was higher compared to pFF from the right ovary. On the contrary, the concentration of Cu and Mn was significantly lower in the left ovary pFF. The concentration of Cu ions was negatively correlated with SOD activity. The CAT activity in pFF form left or right ovary did not show any differences.

PL

Metabolizm oksydacyjny jest niezbędny do produkcji energii na potrzeby gamet i zarodków, co związane jest z wytwarzaniem reaktywnych form tlenu. Antyoksydacyjny mechanizm ochrony enzymatycznej znajduje się w oocytach i zarodkach ssaków, a także w płynie pęcherzykowym. Dodatek świńskiego płynu pęcherzykowego do odżywek hodowlanych korzystnie wpływa na wyniki zapłodnienia i dojrzewania in vitro. Celem badań było określenie aktywności katalazy (CAT) dysmutazy ponadtlenkowej (SOD) i persoksydazy glutationowej (GSH-Px) oraz koncentracji Cu,Mn i Zn wpłynie pęcherzykowym świń pobranym z prawego i lewego jajnika. Ogółem pobrano jajniki od 77 loch w wieku 8 miesięcy.Wewszystkich analizowanych próbkach badane enzymy wykazywały aktywność (24,2; 2,65 10–3 U · l–1 i 525 U · l–1 odpowiednio w przypadku CAT, GSH-Px i SOD) i zawierały jomy Zn, Cu i Mn (odpowiednio 13,8 · 103 mol · l–1; 33,3 · 103 mol · l–1 i 133 · 10–9 mol · l–1). W płynie pęcherzykowym pobranym z lewego jajnika aktywność SOD i GSH-Px była wyższa niż w płynie pęcherzykowym prawego jajnika. Natomiast koncentracja Cu i Mn była istotnie niższa w płynie z lewego jajnika. Koncentracja jonów Cu była skorelowana ujemnie z aktywnością SOD. Aktywność CAT w płynie pęcherzykowym z prawego lub lewego jajnika nie wykazała różnic.