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BACKGROUND AND AIMS: In gonadotrope cells, Wnt/βcatenin signaling regulates SF-1 transcription factor gene activity. SF-1 response elements are found in the promoter regions of four gonadotrope signature genes: LHβ, FSHβ, α-GSU and GnRH-R. β-catenin and DAX-1 act as SF-1 transcriptional coactivator/corepressor, respectively, to affect SF-1 mediated transcription of its target genes. The relationship between hypothalamic GnRH neurons steady-state level and posttranscriptional regulation of transcriptional factors genes activity in anterior pituitary gland remains unknown. METHODS: The present study was to determine whether and how kisspeptin 1–10 and RFPR-3, both known as potent regulators of endogenous GnRH network, affect SF-1/β-catenin/ DAX-1 mRNA stability and what is their impact on SF-1/βcatenin/DAX-1 proteins intracellular expression and localization. 32 female rats received intracerebroventricular pulsatile microinjections of 2 nM GnRH, 1 nM kisspeptin 1–10, 2 nM RFRP-3 and 0.9% NaCl (control). To estimate mRNAs stability, USB poly(A) tail-length Assay Kit (Affymetrics) was used. For protein analysis, double-label immunofluorescence technique was applied. Formalin fixed 4 µm paraffin sections were stained and visualized on a FluoView FV-1000 OLYMPUS confocal microscope. RESULTS: Obtained results indicate that regulatory impact of kisspeptin 1–10 and RFRP-3 on pituitary SF-1/β-catenin/DAX-1 system activity can be exerted via modulation of mRNAs stability. Impact on poly(A) tail length is not related to effects exerted on mRNA expression level. Exogenous kisspeptin, ineffective in SF-1 as well as β-catenin genes transcription stimulation, promoted elongation of their poly(A) tails length. In contrast, exogenous RFRP-3 affected DAX-1 mRNA stability by promotion of poly(A) tail length shortening.
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