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Somaclonal variation at the nucleotide sequence level in rice (Oryza sativa L.) as revealed by RAPD and ISSR markers, and by pairwise sequence analysis

100%

Ngezahayo F. , Dong Y. , Liu B.

Journal of Applied Genetics

2007

tom 48

nr 4

329-336

The nature of somaclonal variation at the nucleotide sequence level was studied in rice cv. Nipponbare. First, we investigated genomic variations by using 2 molecular marker systems: RAPD (random amplified polymorphic DNA) and ISSR (inter-simple sequence repeat). This was followed by sequencing of selected bands that represented genomic variations, and pairwise sequence analysis taking advantage of the whole genome sequence of rice. In addition, transpositional activity of the active MITE, mPing, was analysed by locus-specific PCR amplifications. The 2-year-old calli and their regenerated plants, analysed with 24 RAPD and 20 ISSR primers, showed moderate levels of genomic variation (20.83% and 17.04%, respectively). To test whether DNA methylation plays a role in somaclonal variation, the calli were treated with 5-azacytidine, a chemical agent that reduces cytosine methylation by blocking the activity of DNA methyltransferase. Though dwarfism occurred in regenerants from treated calli (a hallmark of the drug treatment), there was only a slight increase in the frequency of somaclonal variation detected in the treated calli and their regenerated plants relative to untreated controls. The transposon mPing also remained immobile in both treated and untreated calli. Nevertheless, dendrograms constructed according to the Jaccard coefficient calculated by UPGMA of the ISSR bands revealed that the 5-azacytidine-treated and untreated somaclones were grouped into 2 distinct clusters, suggesting a possible indirect effect of the treatment on the genomic changes, depending on the marker used. Sequence analysis indicated a low level of variation (0.31%), with single-base-pair substitutions predominating.

A Study of Monitoring Performances with the INSIDE System

26%

Pennazio F. , Attili A. , Battistoni G. , Belcari N. , Bisogni M. , Camarlinghi N. , Cappucci F. , Cecchetti M. , Cerello P. , Ciciriello F. , Cirrone G. , Coli S. , Corsi F. , Cuttone G. , De Lucia E. , Ferretti S. , Faccini R. , Fiorina E. , Frallicciardi P. , Giraudo G. , Kostara E. , Kraan A. , Licciulli F. , Liu B. , Marafini M. , Marino N. , Marzocca C. , Matarrese G. , Morone C. , Morrocchi M. , Muraro S. , Patera V. , Peroni C. , Piersanti L. , Piliero M. , Pirrone G. , Rivetti A. , Romano F. , Rosso V. , Sala P. , Sarti A. , Sciubba A. , Sportelli G. , Voena C. , Wheadon R. , Del Guerra A.

Acta Physica Polonica A

2015

tom 127

nr 5

1468-1470

The INSIDE collaboration aims to build an on-line hadrontherapy monitoring system, based on a dual-head in-beam PET scanner and a secondary charged particles profiler. In this work preliminary experimental results are presented. The validation of the FLUKA-based Monte Carlo simulation tool is shown together with the expected scanner performances.

The INSIDE Project: Innovative Solutions for In-Beam Dosimetry in Hadrontherapy

26%

Marafini M. , Attili A. , Battistoni G. , Belcari N. , Bisogni M. , Camarlinghi N. , Cappucci F. , Cecchetti M. , Cerello P. , Ciciriello F. , Cirrone G. , Coli S. , Corsi F. , Cuttone G. , De Lucia E. , Ferretti S. , Faccini R. , Fiorina E. , Frallicciardi P. , Giraudo G. , Kostara E. , Kraan A. , Licciulli F. , Liu B. , Marino N. , Marzocca C. , Matarrese G. , Morone C. , Morrocchi M. , Muraro S. , Patera V. , Pennazio F. , Peroni C. , Piersanti L. , Piliero M. , Pirrone G. , Rivetti A. , Romano F. , Rosso V. , Sala P. , Sarti A. , Sciubba A. , Sportelli G. , Voena C. , Wheadon R. , Del Guerra A.

Acta Physica Polonica A

2015

tom 127

nr 5

1465-1467

The INSIDE project addresses the online dose monitoring in particle therapy issue. The proposed detectors exploit the secondary neutral and charged particles emitted during the treatment by the irradiated volumes. The new detection techniques allow to return and online a measurement of the released dose and the Bragg peak position.