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EN
The aim of this study was designing of nanostructured bioelectrodes and assembling them into a biofuel cell with no separating membrane. Carbon nanotubes (CNTs) chemically connected with residues of typical mediators, i.e. ferrocene (Fc) and 2,2'-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) deposited on glassy carbon electrodes (GCE) were found useful as mediators for the enzyme catalyzed electrode processes. The electrodes were in turn covered with glucose oxidase from Aspergillus niger AM-11 and laccase from Cerrena unicolor C-139, respectively, incorporated in a liquid-crystalline matrix. The nanostructured electrode coating with the cubic phase film containing enzymes acted as the catalytic surface for the enzymatic reactions that is oxidation of glucose at anode and reduction of oxygen at cathode. For the system with mediators anchored to CNTs the catalysis was almost ten times more efficient than on bare GCE electrodes: catalytic current of glucose oxidation was 1 mAcm-2 and oxygen reduction current exceeded 0.6 mAcm-2. The open circuit voltage of the biofuel cell was 0.43 V. Application of the carbon nanotubes increased maximum power output of the constructed biofuel cell to 100 \miWcm-2 without stirring the solution. It is ca. 100 times more efficient than using the same bioelectrodes without nanotubes on the electrode surface.
EN
Monitored natural attenuation can be a successful management strategy for groundwater contamination if requisite microbial and geochemical conditions are present. Subsurface microbial ecology of a contaminated aquifer is often investigated by sampling groundwater from the plume. However, the subsurface microbial ecology of a contaminated aquifer is better represented by in situ biofilms than planktonic organisms. Conventional sampling to collect the sessile subsurface microbial community requires coring of the aquifer sediments. However, the efficiency of extracting viable microorganisms and biomarkers from these sediments varies with site geochemistry. Therefore, we have developed a rapid and efficient microbial-sampling system based on Bio-Sep® technology. Bio-Sep® consists of 3-4 mm diameter spherical porous beads engineered from a composite of 25 % aramid polymer and 75 % powdered activated carbon. These beads encourage microbial growth by providing high internal surface area with low-shear conditions, and by concentrating limiting nutrients within the groundwater environment. Biomarkers can be efficiently extracted from Bio-Sep® to provide measures of viable biomass, redox environment, and microbial community composition. We have introduced Bio-Sep® beads into aquifers contaminated with tetrachloroethylene, BTEX, MTBE, and uranium where we used Bio-Sep® to evaluate the effects of potential remediation amendments and to demonstrate in situ biodegradation. This paper presents a review of the Bio-Sep technology, and its applications for documenting subsurface microbial ecology and bioprocesses in contaminated aquifers.
PL
Monitoring fauny bakteryjnej obecnej w wodach podziemnych jest dogodnym narzędziem w ocenie jakości środowiska. Tradycyjne techniki pobierania próbek wód podziemnych oraz osadów wymagają wykonania odwiertów w celu pobrania żyjących w wodzie mikroorganizmów. W pracy przedstawiono szybki i sprawny system pobierania próbek mikrobowych, korzystający z technologii Bio-Sep®, składający się z kulek porowatych o średnicy 3-4 mm, stwarzających dogodne warunki do wzrostu mikroorganizmów. Kulki Bio-Sep®, zostały wprowadzone do wód zanieczyszczonych tetrachloroetylenem, BTEX, MTBE oraz uranem. W pracy zaprezentowano technologię Bio-Sep® oraz jej zastosowanie.
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