Wyniki wyszukiwania - Biblioteka Nauki

1

Influence of age on transmembrane potential and cell surface change of human peripheral blood lymphocytes estimated with fluorescent probes

100%

Witkowski J. M. , Mysliwski A.

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nr 3

2

Influence of cortisone on ultrastructure of the sublingual salivary gland in rats

80%

Kostula A. , Kozlowska K. , Mysliwski A.

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nr 1

PL

Wpływ kortyzolu na ultrastrukturę ślinianki podjęzykowej szczura

3

Anti-tumor action of tumor necrosis factor against Bomirski Ab melanoma in hamsters

80%

Koszalka P. , Szmit E. , Mysliwski A. , Bigda J.

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nr 4

4

Compensatory effect of TNFalpha on low natural killer activity in the elderly

80%

Mysliwska J. , Bryl E. , Trzonkowski P. , Mysliwski A.

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nr 2

EN

Regulatory effect of CD25, an activation antigen the alpha subunit of interleukin 2 receptor (IL2R) on the activity of natural killer (NK) cells was studied in fifty elderly (57-70 years old) and fifty young people (19-35 years old). Cytotoxic NK activity was assessed by 51Cr release assay, the levels of interleukin 2 (IL2) and tumour necrosis factors alpha (TNFalpha) were measured using bioassays and expression of CD16 and CD25 proteins by flow cytometry. Low NK activity in the elderly was associated with decline of full health, lowered serum concentration of IL2 and increased production of TNFalpha during NK reaction. Inhibition of TNFalpha activity by anti-TNF monoclonal antibody suppressed exclusively NK activity of low NK responders. Moreover, stimulation in vitro of blood mononuclear cells, with TNFalpha induced in the elderly low NK responders a significantly higher increase of the CD25 expression on the surface of NK cells as compared with that in the elderly high responders. Since the CD25 molecule constitutes a subunit of the high affinity receptor, binding IL2 to immunocompetent cells, its increased expression on NK cells of low NK responders would enable them to bind even low amounts of the endogenous IL2 available in this group of the elderly. Thus, an overproduction of TNFalpha seems to be a mechanism compensating, in the non-fully healthy elderly, for the decreased IL2 production, promoting efficient cytotoxic reaction.

5

The effect of angiogenesis inhibitor TNP-470 on the blood vessels of the lungs, kidneys and livers of treated hamsters

80%

Mysliwski A. , Kubasik-Juraniec J. , Koszalka P. , Szmit E.

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nr 1

EN

The growth of solid tumours and their metastases is dependent on the development of new blood vessels (angiogenesis). Therefore angiogenesis inhibitors are potential antitumour drugs. In our previous studies it was found that the angiogenesis inhibitor TNP-470 given to transplantable melanoma-bearing hamsters can decrease the rate of the tumour growth, although the survival time of the animals treated was not significantly affected. It was found finally that TNP-470 given in the vicinity of the growing tumour can cause complete remission of the melanoma in hamsters treated in this way. To check what side-effects could be evoked by such treatment, an examination of the morphology of the blood vessels of the lungs, kidneys and livers of the treated animals was carried out. It was found that the angiogenesis inhibitor applied did not cause any changes which could be observed by light and electron microscopes in the structure of the examined blood vessels of the treated animals.

6

CD4plus CD25plus T regulatory cells decrease the cytotoxicity of CD8 HIGHplus T lymhocytes but not that of NK cells against the antigens of the anti-influenza vaccine

70%

Trzonkowski P. , Szmit E. , Mysliwska J. , Dobyszuk A. , Mysliwski A.

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nr Suppl.

7

Thyroid C cell function during fasting and refeeding of young and old rats

70%

Kmiec Z. , Kotlarz G. , Mysliwski A. , Wyrzykowska M. , Pokrywka L.

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nr 2

8

Studies of leptin receptors expression in the hypothalamus fasted and refed young and old rats

61%

Kotlarz G. , Mlotkowska M. , Kmiec Z. , Hinc K. , Kubasik-Juraniec J. , Mysliwski A.

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tom 53

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nr Supplement 1

9

Ultrastructure of the ventromedial hypothalamic nucleus in fasted and refed young and old rats

61%

Kubasik-Juraniec J. , Kmiec Z. , Tukaj C. , Adamowska A. , Kotlarz G. , Pokrywka L. , Mysliwski A.

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nr 2

EN

Many hypothalamic nuclei are involved in the regulation of food intake and energy homeostasis. An ultrastructural investigation of the hypothalamic ventromedial nucleus (VMN), a hypothetical “satiety centre” was performed to explore the morphological basis of altered feeding behaviour of old rats in an experimental model of fasting/refeeding. Young (5 months old, n = 12) and old (24 months old, n = 12) male Wistar rats were fasted for 48 hours, then refed for 24 hours and sampled thereafter. Brain tissue was fixed by perfusion, histological and ultrathin sections were obtained by routine methods. Although food intake was similar in control young and old rats, during refeeding old animals consumed less chow than young ones. The EM analysis of VMN neurones of old control rats revealed, besides typical age-related residual bodies, deep indentations of the nuclear envelope and the presence of long, undulating rough endoplasmic reticulum cisternae in the cell periphery. In both young and old rats fasting for 48 hours led to the expansion of Golgi complexes and increased folds of the nuclear envelope, which is suggestive of enhanced cellular activity of the VMN neurones. These fasting-induced alterations were sustained in the VMN neurones of refed rats in both age groups. The results showed that the VMN neurones of old control rats differ at the ultrastructural level from young ones. However, starvation and subsequent refeeding cause similar alterations in the hypothalamic neurones of “satiety centre” of both young and old rats.

10

The effect of fasting and refeeding on the ultrastructure of the hypothalamic paraventricular nucleus in young and old rats

61%

Kubasik-Juraniec J. , Kmiec Z. , Tukaj C. , Rudzinska-Kisiel T. , Kotlarz G. , Pokrywka L. , Mysliwski A.

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nr 1

EN

In order to explore the morphological basis of the altered feeding behaviour of old rats, an ultrastructural investigation of the magnocellular neurons of the hypothalamic paraventricular nucleus (PVN) was performed. Young and old male Wistar rats, 5 and 24 months old, respectively, and with each age group comprising 12 animals, were divided into 3 groups. The rats in Group I were used as controls (normally fed), the rats of Group II were fasted for 48 hours and in Group III the rats were fasted for 48 hours and then refed for 24 hours. The brains were fixed by perfusion and histological and ultrathin sections were obtained by routine methods. Common features of the magnocellular PVN neurons of young and old rats were abundant Golgi complexes and short fragments of RER localised at the cell periphery. In contrast to young rats, the PVN neurons of old animals showed deep indentations of the nuclear envelope and agerelated residual bodies. In both age groups fasting for 48 hours led to the expansion of the Golgi complexes and dilatation of RER cisternae. In contrast to those in fed rats, RER cisternae in the neurons of old fasted animals were situated between the nuclear envelope and the Golgi zone. Prolonged RER cisternae were distributed in the peripheral cytoplasm of refed old rats. Our observations suggest that at the ultrastructural level the process of ageing does not change the responsiveness of magnocellular PVN neurons to fasting-refeeding.