The development of modern clinical medicine and the truthful medical diagnosis require hard physicochemical data. Furthermore, the social demands for more accurate and collected data, which refer to human health in real conditions and in real time, force the quest for techniques which would be possible to be applied in a broadly understood clinical field. In this article two techniques are described. A high vacuum technique which is an X-ray photoelectron spectroscopy (XPS), and a functional near infrared spectroscopy (fNIRS), which might be applied in vivo condition. For the both techniques, the results pointing their applicability in the clinical and modern biochemical research are described.
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A high level of the nucleotide sequence conservation was found for mitochondrial nad3 gene of carrot. Three silent nucleotide substitutions differentiate nad3 open reading frames from cytoplasmic male sterile and male fertile carrots. All these differences are preserved on the RNA level. Partial and silent editing also distinguished both carrots. Three of the C to U conversions were specific to the fertile line. In the two examined carrot lines editing did not affect the mode of alteration of encoded amino acids.
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