Wyniki wyszukiwania - Biblioteka Nauki

Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl

Ograniczanie wyników

Znaleziono wyników: 2

Liczba wyników na stronie

Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Genetic profiles of bovine herpesvirus 1 strains isolated from cattle in Poland

100%

Rola J. , Zmudzinski J.

2010

tom 54

nr 4

The purpose of the study was to determine subtypes of BHV1 strains isolated from cattle in Poland. In total five different strains of BHV1 were isolated during the last years. All isolates as well as four archival and two reference BHV1 strains were analysed by PCR and restriction enzyme analysis. Specificity of the strains was confirmed by PCR with primers complementary to the nucleotide sequence of gD gene. Subsequently, genomic DNA of the tested strains was digested with endonucleases Hind III and Hpa I. Restriction enzyme analysis revealed that all recently isolated BHV1 strains belonged to the BHV1.1 subtype. Among archival viruses, two strains had restriction pattern similar to the subtype BHV1.1 and two others to the subtype BHV1.2a. The presented study showed that currently subtype BHV1.1 is dominant in cattle population in Poland.

Badania nad określeniem genotypu szczepów terenowych herpeswirusa bydła typ 1 izolowanych od bydła w Polsce

80%

Rola J. , Socha W. , Zmudzinski J.

Medycyna Weterynaryjna

2011

tom 67

nr 02

The aim of this study was to determine the subtype of bovine herpesvirus 1 (BHV1) strains currently circulating in the cattle population in Poland. Altogether 224 nasal swabs, 36 lung tissue samples and 14 samples of fluid after embryo washing in cows were tested by virus isolation test. Five field strains of BHV1 were isolated. These isolates as well as 4 archival and 2 reference strains of BHV1 were subjected to restriction enzyme analysis. Viral DNA was cleaved with Hind III, Hpa I and Pst I enzymes. After digestion with Hind III typical patterns for BHV1.1 subtype were observed in all field strains. Among archival strains 2 belonged to BHV1.1 and 2 others to B-HV1.2a subtype. These findings were confirmed by digestion with other enzymes. The presented study showed that strains of BHV1.1 subtype are dominant in cattle in Poland.