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Hamowanie inwazyjności komórek czerniaka poprzez stosowanie kombinacji siRNA dla N-kadheryny i inhibitorów kinaz białkowych

100%

Ciolczyk-Wierzbicka D. , Gil D. , laider P.

Postępy Mikrobiologii. Suplement

2017

tom 56

nr 2

Oligosaccharide structures of N-cadherin from human normal and cancer bladder cell lines by matrix-assisted laser desorption mass spectrometry

88%

Hoja-Lukowicz D. , Litynska A. , Amoresano A. , Ciolczyk-Wierzbicka D. , Laidler P.

Acta Biologica Cracoviensia. Series Zoologia

2007

tom 49

31-39

Carbohydrate moieties of N-cadherin from human melanoma cell lines

88%

Ciolczyk-Wierzbicka D. , Gil D. , Hoja-Lukowicz D. , Litynska A. , Laidler P.

Acta Biochimica Polonica

2002

tom 49

nr 4

Expression of N-cadherin an adhesion molecule of the cadherin family, in tumor cells is associated with their increased invasive potential. Many studies suggested the role of N-linked oligosaccharides as important factors that contribute to metastasis by influencing tumor cell invasion and adhesion. N-cadherin is a heavily glycosylated protein. We have analysed the carbohydrate profile of this protein synthesized in human melanoma cell lines: WM35 from the primary tumor site and WM239, WM9, and A375 from different metastatic sites. N-cadherin was immunoprecipitated with anti-human N-cadherin polyclonal antibodies. Characterisation of its carbohydrate moieties was carried out by SDS/PAGE electrophoresis and blotting, followed by im- munochemical identification of the N-cadherin polypeptides and analysis of their glycans using highly specific digoxigenin or biotin labelled lectins. The positive reaction of N-cadherin from the WM35 cell line with Galanthus nivalis agglutinin (GNA), Datura stramonium agglutinin (DSA) and Sambucus nigra agglutinin (SNA) indicated the presence of high-mannose type glycans and biantennary complex type oligosac- charides with a2-6 linked sialic acid. N-cadherin from WM239, WM9, and A375 cell lines gave a positive reaction with Phaseolus vulgaris leukoagglutinin (L-PHA) and lotus Tetragonolobus purpureas agglutinin (LTA). This indicated the presence of tri- or tetra-antennary complex type glycans with a-fucose. In addition, N-cadherin from WM9 (lymphomodus metastatic site) and A375 (solid tumor metastatic site) contained complex type chains with 2–3 sialic acid (positive reaction with Maackia amurensis agglutinin — MAA). The results demonstrated that N-glycans of N-cadherin are altered in metastatic melanomas in a way characteristic for invasive tumor cells.

Identification of PNA-positive proteins in the primary uveal melanoma cell line by mass spectrometry

75%

Hoja-Lukowicz D. , Litynska A. , Pochec E. , Przybylo M. , Kremser E. , Ciolczyk-Wierzbicka D. , Laidler P.

Acta Biologica Cracoviensia. Series Zoologia

2006

tom 48

39-47