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Effects of centrifugal stress on cell disruption and glycerol leakage fromDunaliella salina

100%

Xu Y. , Milledge J. J. , Abubakar A. , Swamy R. A. R. , Bailey D. , Harvey P. J.

Microalgae Biotechnology

2015

tom 1

nr 1

Dunaliella salina accumulates large amounts of intracellular glycerol in response to the increases in salt concentration, thus is a potential source for producing fuel grade glycerol as an alternative to biodiesel-derived crude glycerol. D. salina lacks a cell wall; therefore the mode of harvesting Dunaliella cells is critical to avoid cell disruption caused by extreme engineering conditions. This study explored cell disruption and glycerol leakage of D. salina under various centrifugal stresses during cell harvesting. Results show a centrifugal g-force lower than 5000 g caused little cell disruption, while a g-force higher than 9000 g led to ~40% loss of the intact cells and glycerol yields from the recovered algal pellets. Theoretical calculations of the centrifugal stresses that could rupture Dunaliella cells were in agreement with the experimental results, indicating optimisation of centrifugation conditions is important for recovering intact cells of D. salina enriched in glycerol.

Problems and Prospects of Tree Planting in Kano Metropolis, Kano State, Nigeria

84%

Dantani A. , Khalil A. M. , Abanikanda J. O. , Magaji U. A. , Dantani M. , Abubakar A. , Sa’idu M. , Yunus A.

World News of Natural Sciences

2024

tom 53

186-202

The study assessed the problems and prospects of tree planting in Kano metropolis, employing a mixed-methods approach utilizing both primary and secondary data sources. Simple random sampling was used in selecting LGAs, 180 questionnaires were administered and retrieved using proportional allocation. Data screening, organization, and analysis, including descriptive statistics and Chi-square test were conducted using SPSS version 23. The result revealed demographic characteristics of the respondents, indicating that the age group between 20-30 years had the highest response rate, with 64% of respondents being male and 36% female. Additionally, singles constituted the majority of respondents (64.91%), while urban dwellers accounted for 76.61%. The occupational distribution showed that 39% were business people, with farming and civil service having the lowest representation at 15%. In terms of educational qualifications, HND/BSc holders comprised 38% of the respondents, while FSCL had the lowest representation at 4.7%. The research also identified challenges in tree planting among urban dwellers, including inadequate land, tendering to maturity, lack of public enlightenment, low private involvement, poor government policy, poverty, and insufficient funding and planting materials. These challenges were emphasized with a Likert mean score of 3.025. Solutions include initiatives such as tax breaks, subsidies, and green space regulations can incentivize tree planting, while collaborative efforts involving public-private partnerships and community-based education campaigns are vital for widespread awareness and active involvement. Adequate funding remains a linchpin, and its securement through diverse channels ensures the long-term success of tree planting programs. Effective land use planning, incorporating zoning regulations and strategic establishment of nurseries, further contributes to a resilient and green urban environment. By addressing these facets comprehensively, cities can navigate challenges and cultivate thriving urban forests that benefit both the environment and the well-being of their residents.