Wyniki wyszukiwania - Biblioteka Nauki

1

Wartosc diagnostyczna oraz epizootyczne aspekty zastoswoania testow RTCIT i RFFIT w diagnostyce wirusa wscieklizny

100%

Otachel-Hawranek J.

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nr 196

1-100

2

Wykorzystanie probek mleka do kontroli zakazenia stad wirusem bialaczki bydla

100%

Otachel-Hawranek J.

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tom 72

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nr 08

320-322

3

Przydatnosc odczynu syncytialnego do wczesnej diagnostyki zakazen wirusem bialaczki bydla [BLV] u cielat

100%

Otachel-Hawranek J.

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nr 07

315-317

EN

The studies were carrid out on 70 calves coming from two farms in the Wrocław district. The calves from cows with positive serological titers (assessed by AGID) against bovine leukaemia virus (BLV). In the farms under study the last case of nodular leukaemia was observed 7 years before and the results of haematological examinations were within the normal range. Out of 7 calves aged 6 months with the presence of specific antibodies against BLV only in two cases the syncytial test appeared to be positive in previous examinations, i.e. in calves aged 1 month. Besides, the findings of serological examinations performed on calves at the age of six months indicated that the percentage of calves infected with BLV in the prenatal period was slight. Calves from cows with seropositive results can in the majority of cases avoid infection with BLV in the postnatal period by their isolation; the contacts of calves with their mothers ought to be possible only during feeding them with colostrum.

4

Eradication of enzootic bovine leukosis in dairy cattle from the Lower Silesia region

100%

Otachel-Hawranek J.

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tom 51

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nr 4

5

Przydatnosc proby izolacji wirusa wscieklizny na komorkach mysiej neuroblastomy w diagnostyce wscieklizny

100%

Otachel-Hawranek J.

Medycyna Weterynaryjna

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2009

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tom 65

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nr 11

775-780

EN

In the study, the usefulness of rabies virus isolation in neuroblastoma cell culture (RTCIT) as a new method for the diagnosis of rabies was examined. RTCIT results were compared with those obtained by conventional diagnostic methods (FAT and MIT) in terms of their sensitivity in detecting street strains of rabies virus in the brain tissue of suspected animals. The presence of rabies antigen in the brain tissue of animals was confirmed in 93% samples, using FAT and RTCIT, and in 91.6% samples, using MIT. Two results were MIT positive and RTCIT negative and two other results were MIT negative and RTCIT positive. The samples positive only in RTCIT reacted doubtfully in the FAT direct method. Moreover, none of the seven RFFIT-positive highly diluted (10⁻⁴-10⁻⁶) samples of animal brain gave positive reaction in MIT. A thirty-day cycle of observation appeared to be too short for the expression of rabies virus in the infected mice. On the other hand, cytotoxic effect prevented the detection of rabies virus by the RTCIT method in two contaminated samples of brain tissue. The samples were MIT positive. The results of this study indicate that RTCIT was able to detect rabies virus in the brain tissue of rabid animals even when its quantity in the inoculum was very low. Owing to its high sensitivity, RTCIT can replace the mouse inoculation test in routine rabies diagnosis.

6

Uwalnianie stad krow woj.wroclawskiego od enzootycznej bialaczki

100%

Otachel-Hawranek J.

Medycyna Weterynaryjna

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1996

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tom 52

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nr 09

587-590

EN

The purpose of the work was to assess the results of relieving cows from bovine enzootic leukaemia taking into account some laboratory tests, ie agar gel diffusion test, ELISA and syncytial test. In the Wrocław district immunodiffusion testing was introduced to standard practice in 1982. Up to 1990 only a small number of animals (9-14%) were evaluated for leukaemia and the percentage of positive seroreactions ranged from 27-7%. The process of the spreading of the EBL virus at that time was significant and was confirmed by positive results of the virus isolation from the peripheral blood of cows and calves coming from herds being treated for enzootic leukaemia. In 1991-1995 mass serological examinations involved the majority of cattle and the percentage of positive results declined to 5.8% in 1991 and 1.67% in 1995. In light of the findings the best results were obtained when the serological tests were introduced as obligatory ones and the cost of the studies were covered by Ministry of Agriculture.

7

Reproductive losses in dairy cattle infected with BVD-MD virus - a field study

100%

Otachel-Hawranek J.

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nr 4

8

Wstepna obserwacja nad zastosowaniem testu ELISA do diagnostyki wiosennej wiremii karpi

63%

Niemczuk W. , Otachel-Hawranek J.

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nr 06

225-227

9

Przydatność odczynu syncytialnego w diagnostyce zakażeń wirusem białaczki (BLV) u krów w okresie okołoporodowym oraz ich potomstwa

63%

Otachel-Hawranek J. , Boruta J.

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tom 46

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nr 10

EN

The studies were carried out on 15 cows being at the 9th month of pregnancy and on their offspring. The newborn calves were fed on mothers’ milk. The cows were selected on the basis of positive immunodiffusion tests (ID). The studies comprised haematological, serological and virological tests, i.e. the isolation of bovine leukaemia virus on spleen foetus cells of cattle. In the light of the studies the syncytial test cannot be regarded as the only method in the diagnosis of early infections in cattle. The positive result of the test is dependent to much extent on the number of lymphocytes in the peripheral blood of animals.

10

Izolacja wirusa wscieklizny na komorkach mysiej neuroblastomy

63%

Molenda J. , Otachel-Hawranek J.

Medycyna Weterynaryjna

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1998

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tom 54

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nr 12

823-825

EN

Two official methods are most commonly employed in laboratory proceedings aimed at confirming the existence of rabies in animals: the fluorescent antibody test (FAT) and mouse innoculation test. Introducing the rabies tissue culture infection test (RTCIT), that serves to multiply the virus into cell culture may improve and reduce laboratory procedure time. In our examination we managed to detect the virus in naturally and experimentally infected animals. The method proved to be as efficient as the mouse inoculation test, but its results could be obtained more rapidly. The advantages and limitations of the test are discussed in this article.

11

Identyfikacja antygenu wirusa BVD-MD u zakazonego bydla przy uzyciu immunoenzymatycznego testu ELISA

51%

Otachel-Hawranek J. , Krolinski J. , Kosicki M.

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tom 60

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nr 07

728-731

12

Uwalnianie zarodkow bydlecych od kruszywa komorkowego uzyskanego z wypluczyn z macicy krow zakazonych wirusem BL

51%

Krolinski J. , Lukaszewski Z. , Marcinkowski K. , Otachel-Hawranek J.

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tom 51

113-116

13

Wykorzystanie transferu zarodkow bydlecych do uzyskiwania zdrowego potomstwa od bialaczkowych matek

51%

Krolinski J. , Marcinkowski K. , Lukaszewski Z. , Otachel-Hawranek J.

Medycyna Weterynaryjna

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1992

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tom 48

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nr 02

79-81

EN

The purpose of the work was to confirm the possibility of embryo transfer to protect offspring of leukaemic cows of high breeding value from infection with EBLV. The studies were carried out on 229 donors of embryos infected with the EBLV. Heifers free from enzootic leukaemia were receivers of the embryos. The disease was diagnosed both in the donors, receivers and new-born calves by the gel precipitation test or ELISA. From 152 donors 1390 embryos were obtained and only 676 (48.6%) were qualified as suitable. There were transfered 585 embryos resulting in 278 pregnant cows (the efficacy of the transfer was 47.5%). There were born 274 calves free from leukaemia and 4 died during parturition. The transferes and new-born calves were housed in isolation under conditions which protected them against EBLV. The studies showed that the transfer of embryos was an effective method allowing to get healthy offspring from leukaemic cows on condition that strict prophylactic measures during the operation and pregnancy and after calving were applied.

14

Przydatnosc testu ELISA do wykrywania przeciwcial przeciw wirusowi EBB w pojedynczych i zbiorczych probach mleka

38%

Otachel-Hawranek J. , Korfanty E. , Szachnowski S. , Erbert M. , Ciszewski G. , Jakubiak J.

Medycyna Weterynaryjna

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1995

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tom 51

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nr 07

402-404

EN

The aim of the studies was to determine the usefulness >f the ELISA test to detect antibodies against EBB virus n the milk of cows from the Wroclaw voivodship, where he test is to be used for the routine examination of milk. The results of this test were compared with blood sera ;xaminations by the ID test. The studies were done on 29 nilk samples including 17 bulk milk samples. A kit of ,Leukotest” acc. to Bommeli was used. Except for 4 cases, he results of both tests were identical. Positive results in he ELISA test obtained with milk samples negative in he ID test point to a high sensitivity of ELISA. The results obtained confirmed the usefulness of the ID :est with blood sera to detect and control EBB. On the 'arms where the program of EBB eradication has been ntroduced, the latest negative results in the ID test have jeen in most cases confirmed by ELISA. ELISA, as one )f the serological diagnostic methods, can be used to :xamine bulk milk in large farms free of EBB.