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EN
This paper presents the analysis of the effect of differential signal coupler positioning accuracy on its high frequency performance parameters for contact-less high speed chip-to-chip data transmission on PCB application. Our considerations are continuation of the previous works on differential signal coupler concept, design methodology and analysis for high speed data transmission monitoring. The theoretical analysis of possible coupler positioning innaccuracies is extended for representative design cases by simulations carried out using EM simulator. The results reported here confirm that the concept of contactless monitoring of high speed chip-to-chip data transmission in a pair of coupled microstrip lines is of practically usefulness without applying the expensive and precise positioning system.
EN
This paper presents a technique of decoupling differential signals transmitted in a pair of microstrip lines on a printed circuit board (PCB), using dedicated coupler for high speed data transmission monitoring in chip-to-chip interconnections. The coupler used for signal probing is overlayed on the pair of microstrip lines under test, and provides a signal to the next blocks of the measurement system without disturbing transmission in the lines. Starting from the basic configuration obtained in the previous work, we describe a procedure of re-designing the coupler for enhanced directivity so that it is suitable for high-speed data transmission monitoring. The new coupler structure has improved topology based on stepped-impedance resonators with geometry parameters adjusted using numerical optimization techniques. The correctness of the design has been confirmed by the time-domain measurements performed for real chip-to-chip communication signals, using a dedicated measurement system based on Xilinx Virtex-6 FPGA Development Kit. The presented technology can be used for reducing the cost of the product diagnostics by eliminating dedicated measurement connectors, run-time system debugging or reverse engineering.
EN
Experiments were performed on 180 mice from two lines dubbed light (L) and heavy (C), selected divergently for body weight over 108 generations. The main hypothesis was that the changes occurring in body weight and muscle weight as a result of directed divergent selection could be associated with changes in the transcription of some miogenic genes and/or with the level of proteins regulating myogenesis and with the composition of muscle fibres.Hind limb muscle masses from females and males of the two lines were weighed at 1 and 3 weeks and 3 month of age. Morphological analysis for histological cross-sections of the gastroenemius muscle was carried out in 3-week and 3-month-old mice. The percentage comparison of nuclei in muscle fibres were analysed, too. Levels of MYOD1, MYF-5 and myogenin at the same time points were determined using Western blotting. Microsatellite markers for MyoD1, Myf-5 and MYOG were used to compare allele frequencies of analysed genes in both lines. There were differences in muszle weight between the sexes at age of 3 months. Muscles of the hind limbs were heavier in males than in females by 23.7% in line C, and by 14% in line L. Significant differences in muscle mass were accompanied by changes in muscle fibre size. The number of large-diameter muscle fibres increased with animals’ age, and in females fibres of diameters of 60-80 μm accounted for 38% of the total in line C, as compared with 94% preponderance of smaller (20-60 μm) fibres in the muscle of line L females. The numbers of nuclei were clearly greater in line C than in line L individuals, as well as in 3-month-old animals as compared with those at 3 weeks of age.Selection have brought about change, not only in myogenesis, but also in the frequency of alleles of microsatellite markers MyoD1, Myf-5 and at the myogenin locus, thus suggesting that molecular differences between the lines have arisen. Differences in the levels of MYOD1, MYF5 and MYOG are evident between both sexes and the selected lines of mice.
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