Wyniki wyszukiwania - Biblioteka Nauki

1

Plant protoplast technology: current status

100%

Davey M. R. , Anthony P. , Lowe K. C. , Power J. B.

Acta Physiologiae Plantarum

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2004

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tom 26

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nr 3 suppl.

2

Agrobacterium-mediated transformation of Lupinus mutabilis L. using shoot apical explants

100%

Babaoglu M. , McCabe M. S. , Power J. B. , Davey M. R.

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tom 22

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nr 2

3

Genetic transformation of wheat: progress during the 1990s into the Millennium

100%

Ingram H. M. , Livesey N. L. , Power J. B. , Davey M. R.

Acta Physiologiae Plantarum

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2001

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tom 23

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nr 2

4

Plant protoplast technology: current status

100%

Davey M. R. , Anthony P. , Power J. B. , Lowe K. C.

Acta Physiologiae Plantarum

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2005

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tom 27

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nr 1

EN

Robust and reproducible protoplast-to-plant systems are crucial for underpinning genetic manipulation technology involving somatic hybridisation and transformation. Novel and effective approaches for maximising the efficiency of such protoplast cultures include supplementation of media with surfactants and artificial gas carriers, such as perfluorochemicals and haemoglobin. Physical parameters, particularly electrostimulation, also enhance the development of protoplasts and protoplast-derived cells in cullure. DNA uplake into protoplasts is now a routine and universally accepted procedure in plant biotechnology for introducing and evaluating both short-term (transient) and long-term (stable) expression of genes in cells and regenerated plants. Imporlantly, protoplast fusion overcomes pre- and post-zygotic sexual incompatibility barriers and generates novel germplasm through new nuclear-cytoplasmic combinations. In this respect, considerable progress has been made in generating somatic hybrid plants, particularly in citrus, brassicas and polato. Isolated protoplasts are also a unique single cell syslem for evaluating aspects of ultrastructure, genetics and physiology, with potential for the biosynthesis of novel secondary products, including commercially-important recombinant proteins (e.g. antibodies), and as systems in toxicity screening. Recent advances in protoplast technology have benefited from advances in animal and microbial cell culture, with interesting parallels existing between these systems. Further innovations will necessitate the strengthening of interdisciplinary links in these research fields and the requirement for continued dialogue and cooperation between workers with diverse but complementary skills.

5

Agrobacterium-mediated transformation of Bangladeshi Indica rices

88%

Al-Forkan M. , Power J. B. , Anthony P. , Lowe K. C. , Davey M. R.

Cellular and Molecular Biology Letters

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2004

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tom 09

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nr 2

EN

Morphologically normal, fertile transgenic plants were obtained by co-culturing embryogenic calli of the Bangladeshi Indica rice cultivars BR26 and Binni with Agrobacterium tumefaciens strain LBA4404 carrying the super binary vector pTOK233. Acetosyringone (100 μM) in the medium during coculture (25-28°C) and selection on hygromycin B (50 mg 1-1) were essential for efficient transformation. Stable integration and expression of β-glucuronidase, neomycin phosphotransferase and hygromycin phosphotransferase genes in regenerated plants were confirmed by histochemical and fluorometric assays, ELISA and Southern analysis. Two to 3 copies of T-DNA were integrated into regenerated plants; transgene expression did not correlate with gene copy number. Mendelian segregation of transgenes occurred in T1 seed progeny.

6

Culture treatments for enhancing post-thaw recovery of cryopreserved suspension cells of potato cv. desiree

88%

Sadia B. , Anthony P. , Lowe K. C. , Power J. B. , Davey M. R.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 4

EN

An efficient and reproducible protocol has been developed for the cryopreservation of cell suspension cultures of the potato (Solarium tuberosum L.) cv. Desiree. An evaluation was made of the effectiveness of different pre-culture and post-thaw treatments on cell growth, as measured by changes in biomass. Cell suspensions were cultured in UM medium supplemented with 0.25, 0.5, 0.625, 0.75 or 1.0 M sucrose prior to cryopreservation. Sucrose-treated cells were harvested from suspension and 0.75 ml packed cell volumes placed in 2 ml capacity polypropylene vials with 0.5 ml of chilled cryoprotectant (glycerol 46.0 g 1-1, dimethylsulphoxide 39.0 g 1-1, sucrose 342.0 g 1-1 proline 5.0 g 1-1; pH 5.8). Cells were frozen at -0.5°C min-1 from 0 to -35°C, held at -35°C for 35 min and stored, for 10 days, in liquid nitrogen (-196°C). The most effective pre-treatment, in terms of subsequent post-thaw cell viability as assessed by fluorescein diacetate uptake or triphenyltetrazolium chloride reduction, was culture with 0.75 M sucrose. For this treatment, the mean absorbance (490 nm) following triphenyltetrazolium chloride reduction was 88% greater (p < 0.05) than control and 59% greater (p < 0.05) for thawed cells also cultured on supporting filter paper discs.

7

Magnetic levitation, gravity and plant genomics

75%

Davey M. R. , Hampp R. , Martzivanou M. , Anthony P. , Capdefier C. , Power J. B. , Lowe K. C.

Acta Physiologiae Plantarum

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2004

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tom 26

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nr 3 suppl.