Wyniki wyszukiwania - Biblioteka Nauki

1

Diagnostyka i leczenie choroby von Willebranda u psów

100%

Bulc M.

Weterynaria w Praktyce

|

2014

|

tom 11

|

nr 03

2

Toksyczność ksylitolu u psów

100%

Bulc M.

Weterynaria w Praktyce

|

2014

|

tom 11

|

nr 11-12

3

100-lecie przyznania Nagrody Nobla za odkrycie insuliny. Słodko-gorzka historia odkrycia insuliny

100%

Bulc M.

Życie Weterynaryjne

|

2023

|

tom 98

|

nr 12

4

Insulina i jej syntetyczne analogi oraz ich zastosowanie w leczeniu cukrzycy psów i kotów

100%

Bulc M.

Weterynaria w Praktyce

|

2014

|

tom 11

|

nr 01-02

5

Innervation of the pineal gland in the Arctic fox (Vulpes lagopus) by nerve fibres immunoreactive to substance P and calcitonin gene-related peptide

63%

Bulc M. , Lewczuk B.

Folia Morphologica

|

2019

|

tom 78

|

nr 4

EN

Background: The study demonstrates, for the first time, the presence of substance P (SP) and calcitonin gene-related peptide (CGRP) in the nerve fibres supplying the pineal gland in the Arctic fox. Materials and methods: The expression and distribution pattern of the studied substances were examined by double-labelling immunofluorescence technique. Results: The SP-positive fibres enter into the pineal gland through the capsule as the nervi conarii. The fibres formed thick bundles in the capsule and connective tissue septa, from where they penetrated into the pineal parenchyma. Inside the parenchyma, the nerve fibres created basket-like structures surrounding clusters of pinealocytes. The density of intrapineal SP positive fibres was slightly higher in the distal and middle parts of the gland than in the proximal one. Double immunostaining with antibodies against SP and CGRP revealed that the vast majority of SP positive fibres were also CGRP positive. The fibres showing a positive reaction to SP and negative to CGRP were scattered within the whole gland. The fibres immunopositive to CGRP and immunonegative to SP were not observed. In the habenular and posterior commissural areas adjoining to the pineal gland the immunoreactive nerve fibres were not found. Moreover, no immunopositive cell bodies were observed in both the pineal gland and the commissural areas. Conclusions: These results reveal that SP and CGRP are involved in the innervation of pineal gland in carnivores. In turn we suggest that these peptides can regulate/ /modulate melatonin secretion. (Folia Morphol 2019; 78, 4: 695–702)

6

Rodzinna arytmogenna kardiomiopatia prawej komory u psów rasy bokser

63%

Bulc M. , Szuster A.

Weterynaria w Praktyce

|

2020

|

tom 17

|

nr 10

7

Dobowe zmiany stezenia melatoniny w osoczu krwi niedojrzalych plciowo loszek podczas dlugiego dnia swietlnego

51%

Lewczuk B. , Bulc M. , Przybylska-Gornowicz B.

Medycyna Weterynaryjna

|

2006

|

tom 62

|

nr 10

1171-1175

EN

The level of melatonin in plasma was investigated in gilts reared during long summer days (sunrise 430, sunset 2030). Lighting in the animal's rooms was provided by windows and fluorescent lamps, which were automatically turned on at 545 and turned off at 1945. The light intensity during photophase was 500 lux at the level of the animal's eyes. Blood samples were taken from twelve gilts over a 24 hour period and further sampling was continued in six pigs over the following 3 days. The effect of exposure to a 500 lux light during the night on plasma melatonin levels was studied in the remaining six gilts. The concentration of melatonin in the plasma was measured by direct radioimmunoassay employing an R/R/19540-16876 antibody and iodinated tracer. Concentrations of the investigated hormone were low during day and rapidly increased at the onset of night during which the plasma melatonin level remained at an elevated plateau and then declined at the onset of day. The mean concentration of plasma melatonin was about three times higher during the night compared to daytime. Monitoring of the pineal hormone level in blood plasma over four consecutive days indicated regular melatonin rhythms in 5 of the 6 investigated pigs. A 500 lux light (turned on for one night) did not prevent a nocturnal increase in plasma melatonin levels.

8

Calcium ions in the pig pineal gland - an ultracytochemical study

51%

Lewczuk B. , Bulc M. , Prusik M. , Przybylska-Gornowicz B.

Journal of Elementology

|

2007

|

tom 12

|

nr 4

335-346

EN

The aim of the study was to analyze the distribution of calcium ions in the pig pineal gland at the level of electron microscopy. The investigations were performed on the pineals obtained immediately after slaughter (performed between 11:30 and 12:00 a.m.) from 4-month-old gilts. The fixation procedures were conducted with the use of pyroantimonate, which reacted with calcium ions and formed electron dense precipitates. The precipitates were found both in the intercellular spaces and in cells - pinealocytes, gial cells, endothelial cells and fibroblasts. The precipitates were much more numerous in the intercellular spaces than in the cells. Amount and distribution of precipitates differed significantly between pinealocytes, therefore two types of cells were distinguished. The first type of pinealocytes included cells containing a small or moderate amount of precipitates. They were usually characterized by light or dark cytoplasm and large variability in number and structure of dense bodies. Pinealocytes classified to the second type possessed large or very large content of precipitates. These cells were characterized by electron dense cytoplasm and showed the presence of numerous dense bodies. In both types of pinealocytes, precipitates were present in the nucleus and in the cytoplasm. In nuclei, precipitates were numerous in nucleoplasma and rather infrequently noted between membranes of the nuclear envelope. In the cytoplasm deposits were found in mitochondria, vesicles and cisterns of smooth endoplasmic reticulum, in the Golgi apparatus and in cytosol. The amount of precipitates in glial cells, endothelial cells and fibrocytes was lower than in pinealocytes.

PL

Celem pracy była analiza ultracytochemiczna rozmieszczenia jonów wapniowych w szyszynce świni domowej. Badano szyszynki pobrane bezpośrednio po uboju (wykonanym między godz. 11:30 a 12:00) od loszek w wieku ok. 4 miesięcy. Do utrwalania gruczołów zastosowano pyroantymonian potasu, co umożliwiło związanie jonów wapniowych w postaci elektronowo gęstych precypitatów, których rozmieszczenie określono za pomocą mikroskopu elektronowego. Precypitaty występowały w przestrzeni międzykomórkowej oraz w komórkach: pinealocytach, komórkach glejowych, komórkach śródbłonka naczyń włosowatych i fibrocytach. Zawartość złogów pyroantymonianu wapnia była znacznie większa w przestrzeni zewnątrzkomórkowej niż wewnątrz komórek. Ze względu na ilość i lokalizację precypitatów możliwe było wyróżnienie dwóch typów pinealocytów. Pierwszy z nich stanowiły komórki zawierające małą lub średnią ilość precypitatów. Komórki te charakteryzowały się elektronowo jasną lub elektronowo gęstą cytoplazmą, a skład ciałek gęstych był zróżnicowany pod względem ilościowym i jakościowym. Drugi typ pinealocytów stanowiły komórki z dużą lub bardzo dużą zawartością precypitatów. Najczęściej charakteryzowały się one elektronowo gęstą cytoplazmą oraz obecnością bardzo licznych ciałek gęstych. W obu typach pinealocytów precypitaty występowały zarówno w jądrze komórkowym, jak i w cytoplazmie. W jądrze stosunkowo liczne precypitaty o zróżnicowanych wymiarach stwierdzono w obrębie chromatyny, natomiast jedynie sporadycznie obserwowano je w przestrzeni między błonami otoczki jądrowej. W cytoplazmie precypitaty występowały w mitochondriach, pęcherzykach i cysternach siateczki śródplazmatycznej gładkiej, strukturach aparatu Golgiego oraz w cytoplazmie podstawowej. Zawartość precypitatów w komórkach glejowych, komórkach środbłonka oraz fibrocytach była znacznie mniejsza niż w pinealocytach.

9

The role of nesfatin - 1 in metabolism regulation: an overview

51%

Bulc M. , Gonkowski S. , Calka J.

Annals of Animal Science

|

2013

|

tom 13

|

nr 2

10

Calcium concrements in the pineal gland of the Arctic fox (Vulpes lagopus) and their relationship to pinealocytes, glial cells and type I and III collagen fibers

45%

Bulc M. , Lewczuk B. , Prusik M. , Gugolek A. , Przybylska-Gornowicz B.

Polish Journal of Veterinary Sciences

|

2010

|

tom 13

|

nr 2

11

Immunohistochemical evidence of the co-localisation of cocaine and amphetamine regulatory peptide with neuronal isoform of nitric oxide synthase, vasoactive intestinal peptide and galanin within the circular muscle layer of the human caecum

45%

Bulc M. , Gonkowski S. , Landowski P. , Kaminska B. , Calka

Folia Morphologica

|

2015

|

tom 74

|

nr 2

EN

The enteric nervous system consists of about one hundred million of neurons. In big mammals (including humans) intestinal enteric neuronal cells are grouped into three types of intramural ganglia located within myenteric, as well as outer and inner submucosal plexuses, which are connected by numerous nerve fibres. Both nerve fibres and cell bodies located in the gastrointestinal tract utilise a broad spectrum of active substances. One of them is cocaine- and amphetamine-regulated transcript peptide (CART). The goal of the current study was to determinate the distribution and degree of co-localisation of CART with substances taking part in intestinal motor activity by double labelling immunofluorescence technique. During the study CART-, neuronal isoform of nitric oxide synthase (nNOS)-, vasoactive intestinal peptide (VIP)- and/or galanin (GAL) — like immunoreactive (LI) nerve fibres in the circular muscle layer of the human caecum were observed in all patients studied. The degree of co-localisation of particular substances with CART depended on their type. The majority of CART-LI fibres contained simultaneously nNOS, slightly lower degree of co-localisation was observed in the case of the VIP, while simultaneously CART- and GAL-positive nerve fibres were observed less often. (Folia Morphol 2015; 74, 2: 176–182)