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Content available Hantavirus RNA not detected in Ixodes ricinus ticks
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A total of 123 water samples were examined in parallel by culture and semi-nested PCR for the presence of Legionella. They comprised: 35 samples of hot water distributed by the urban municipal water supply system (MWSS) taken in institutions, 45 samples of hot water distributed by urban MWSS taken in dwellings, 27 samples of cold water distributed by rural MWSS taken in dwellings, and 16 samples of cold well water taken in rural areas. The greatest frequency of the isolation of Legionella by culture (88.6%) was recorded in the samples of hot water from the urban institutions, having been greater compared to all other sources (p<0.001). The frequency of Legionella isolation from hot water in urban dwellings (28.9%) was significantly greater compared to the combined value (2.3%) for cold water from rural MWSS and wells (p<0.001). Strains belonging to Legionella pneumophila serogroups 2-14 predominated in the examined samples, while strains of L. pneumophila serogroup 1 and strains of Legionella spp. (other than L. pneumophila) were 3-fold less numerous. The rates of positive findings in the semi-nested PCR (stage 2) were greater than culture isolations in all kinds of samples, except for urban institutions. The correlation between the culture and PCR results was positive for samples of hot water from urban MWSS (p<0.01), but not for samples of cold water from rural MWSS and wells (p>0.5). A significant correlation was found between rates of PCRpositive results and numbers of Legionella pneumophila serogroups 2-14 strains, but not for other Legionella serogroups or species. In conclusion, our results support the opinion that though PCR cannot be a substitute for the isolation of Legionella by culture, it could be regarded as an useful complementary method.
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A total of 190 Dermacentor reticulatus ticks (80 males, 110 females) collected on the territory of Ostrów Lubelski, Suchawa, Zalutyń and Kazimierz Dolny (Lublin Province, eastern Poland) were examined by reverse transcription PCR and nested PCR methods for the presence of hantavirus RNA. None of the examined Dermacentor reticulatus specimens showed the presence of the hantavirus-specific RNA in spite of using two pairs of primers and the clearly positive results obtained with the positive control. Thus, the hypothesis about the possible participation of ticks in the transmission of hantaviruses was not confirmed.
EN
Among various species of parasitic protozoans which may contaminate drinking water, Toxoplasma gondii is of a special importance due to the high incidence of infections with this parasite noted in animals and humans. The objective of this study was to determine the frequency of occurrence of T. gondii in drinking water on farms in the area of the Lublin province (eastern Poland) with respect to health risk among the inhabitants, and to assess the role of water in the transmission of Toxoplasma infections in the rural environment. Studies were conducted on 87 farms located in the Lublin province, 14 of which were classified as possessing a good hygienic state, and 73 as possessing a poor hygienic state. A total number of 114 drinking water samples were taken, 80 samples from shallow household wells with a windlass, 16 from deep wells with a pump, and 18 from the water supply system. In microscopic and PCR examinations of 114 water samples, T. gondii was found in 15 (13.2%) and 31 (27.2%) of samples, respectively. The presence of T. gondii DNA detected by PCR test was found significantly more frequently in water samples from the shallow windlassoperated wells than in those from deep wells (p<0.05) and water supply system (p<0.01). Water samples collected from shallow wells located on farms of poor hygienic state contained significantly more frequently DNA of T. gondii than samples from shallow wells located on farms of good hygienic state (43.1% vs. 13.3%, p<0.05). In 26.3% of water samples, oocysts of other protozoans were found belonging to Isospora, Eimeria, and Cryptosporidium. Serologic examinations for the presence of anti-Toxoplasma antibodies conducted among 99 inhabitants of the farms where household wells were used showed 64.6% of seropositive results in IgG class antibodies and 1.0% in IgM class antibodies. Clinical cases of toxoplasmosis were also noted. In the total population examined, a positive correlation was observed between the consumption of unboiled well water and the presence of antibodies against T. gondii (p<0.05), this correlation being especially strong on farms of poor hygienic state enclosing shallow wells (p<0.001). In conclusion, the recorded presence of T. gondii in well water provides an evidence of the potential risk of waterborne infection for humans and animals. Therefore, it seems necessary to implement prophylactic actions on the endangered farms.
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