Wyniki wyszukiwania - Biblioteka Nauki

1

Zastosowanie analizy makrorestrykcyjnej i reakcji PCR w odroznianiu bezplazmidowych szczepow Bacillus anthracis od szczepow przejsciowych [Bacillus sp. Ba 813]

100%

Niemcewicz M. , Bartoszcze M.

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tom 62

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nr 06

658-662

EN

The aim of the study was to use selected genetic analysis to differentiate plasmid cured strains of Bacillus anthracis from transitional strains (Bacillus sp. Ba 813). Two different research techniques (macro restriction analysis and PCR) were used and they confirmed that the PCR technique facilitated detecting the presence of the Ba 813 chromosomal sequence in both strains (plasmid cured Bacillus anthracis strain and Bacillus sp. Ba 813). This result indicated that applying the PCR method to differentiate plasmid cured Bacillus anthracis strains from Bacillus sp. Ba 813 was not possible. The macro restriction method, however, facilitated a precise differentiation of plasmid cured Bacillus anthracis strains from transitional Bacillus sp. Ba 813.

2

Zastosowanie analizy makrorestrykcyjnej do roznicowania wybranych szczepow z grupy Bacillus cereus

100%

Niemcewicz M. , Bartoszcze M.

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tom 62

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nr 09

1065-1070

EN

The aim of the study was to apply macro-restriction analysis in order to differentiate selected strains of the Bacillus cereus group. Three different restriction enzymes (SfiI, NotI and SmaI) were used during the research process and it was confirmed that SmaI is the best restriction enzyme applied in macrorestriction analysis of the Bacillus cereus group. The study used 89 Bacillus cereus group strains, and an identical restriction pattern generated by restriction enzymes was confirmed among 23 of the studied Bacillus anthracis strains. Two groups of strains (four and two strains) among 19 Bacillus sp. Ba 813 and three groups (three, two and two strains) among 44 strains of Bacillus thuringiensis showed identical restriction profiles. The similarity of studied strains was ascertained on the basis of the Dice coefficient, and confirmed that strains showing the same restriction profiles were identical (100% similarity). The similarity between the remaining strains of the Bacillus cereus group and the Bacillus anthracis strains oscillated between 20% to 78.6%, while the similarity of these strains to each other oscillated from 15.4 % to 100%. Dendrograms were also constructed using the UPGMA method.

3

The evaluation of methods for detection of Bacillus anthracis spores in artificially contaminated soil samples

80%

Bielawska-Drozd A. , Niemcewicz M. , Bartoszcze M.

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nr 1

5-10

EN

The nested PCR has been used to evaluate the usefulness and efficiency of different Bacillus anthracis spore isolation methods in contaminated soil samples. The best results were obtained using two methods described by Beyer et al. [1] and Cheun et al. [9]. Outer and inner pairs of primers were designed from the protective antigen gene of plasmid pXO1 as well as from genes B and C of the capsule region of the plasmid pXO2. The influence of soil types on obtained results was also studied. The type of soil samples did not affect the nested PCR results. Furthermore, the sensitivity of nested PCR and PCR – ELISA was also examined.

4

Nowe możliwości hamowania infekcji B. anthracis

80%

Zakowska D. , Bartoszcze M. , Niemcewicz M. , Bielawska-Drozd A.

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2011

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tom 67

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nr 10

XX

To date, antibiotics have been the primary drugs used in the treatment of anthrax infections. However, their effectiveness is questionable, especially during the phase of intensive toxin production in the course of infection, and the number of drug-resistant strains continues to rise. Successful treatment of anthrax infection is therefore becoming more difficult. The article discusses some of the new methods of inhibiting anthrax infections: the inhibition of spore germination and of the attachment of PA to the host cell receptor, the inhibition of the enzymatic process of cleaving PA into PA63 and PA20, and of PA63 oligomerization, endocytosis and translocation (their influence on the protection of macrophages against lysis was also discussed). In addition, the neutralization of B. anthracis LeTx and EdTx toxins was presented as another potential method of inhibiting anthrax infections.

5

Biological agents database in the armed forces

80%

Niemcewicz M. , Kocik J. , Bielecka A. , Wiercinski M.

Archivum Immunologiae et Therapiae Experimentalis

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2014

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tom 62

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nr 5

6

Utilisation of peptides against microbial infections – a review

71%

Mirski T. , Niemcewicz M. , Bartoszcze M. , Gryko R. , Michalski A.

Annals of Agricultural and Environmental Medicine

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2018

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tom 25

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nr 2

7

Brucella - virulence factors, pathogenesis and treatment

71%

Glowacka P. , Zakowska D. , Naylor K. , Niemcewicz M. , Bielawska-Drozd A.

Polish Journal of Microbiology

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2018

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tom 67

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nr 2

EN

Brucellae are Gram-negative, small rods infecting mammals and capable of causing disease called brucellosis. The infection results in abortion and sterility in domestic animals (sheeps, pigs, rams etc). Especially dangerous for humans are: Brucella melitensis, Brucella suis, Brucella abortus, and Brucella canis that trigger unspecific symptoms (flu-like manifestation). Brucella rods are introduced via host cells, by inhalation, skin abrasions, ingestion or mucosal membranes. The most important feature of Brucella is the ability to survive and multiply within both phagocytic and non-phagocytic cells. Brucella does not produce classical virulence factors: exotoxin, cytolisins, exoenzymes, plasmids, fimbria, and drug resistant forms. Major virulence factors are: lipopolysaccharide (LPS), T4SS secretion system and BvrR/BvrS system, which allow interaction with host cell surface, formation of an early, late BCV (Brucella Containing Vacuole) and interaction with endoplasmic reticulum (ER) when the bacteria multiply. The treatment of brucellosis is based on two-drug therapy, the most common combinations of antibiotics are: doxycycline with rifampicin or fluoroquinolones with rifampicin. Currently, also other methods are used to disrupt Brucella intracellular replication (tauroursodeoxycholic acid or ginseng saponin fraction A).

8

New aspects of the infection mechanisms of B. anthracis

71%

Zakowska D. , Bartoszcze M. , Niemcewicz M. , Bielawska-Drozd A. , Kocik J.

Annals of Agricultural and Environmental Medicine

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2012

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tom 19

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nr 4

9

Injuries caused by sharp instruments among healthcare workers – international and Polish perspectives

61%

Goniewicz M. , Wloszczak-Szubzda A. , Niemcewicz M. , Witt M. , Marciniak-Niemcewicz A. , Jarosz M. J.

Annals of Agricultural and Environmental Medicine

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2012

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tom 19

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nr 3

EN

Health care workers (HCW) worldwide are especially exposed to injury by sharp instruments in the course of their duty. The most often executed procedures with injury risk are: intramuscular or subcutaneous injection (22%), taking blood samples, or during intravenous cannulation (20%), and repeatedly replacing the cap on an already used needle (30%). Even a minor sharp injury with only a small loss of blood carries the risk of transfer of over 20 pathogens: Hepatitis B Virus (HBV), Hepatitis C Virus (HCV), HIV/AIDS virus, malaria, syphilis, tuberculosis, brucellosis, herpes virus and diphtheria. The World Health Organization (WHO) estimates that more than two million health care workers experience the stressful event of a percutaneous injury with a contaminated sharp object each year (25-90% of them, however, remain unreported). These exposures result in about 16,000 infections with HCV, 66,000 with HBV and about 1,000 (200-5000) with HIV, which lead to about 1,100 deaths or significant disability. Exposures to sharp injuries and their consequences are highly preventable through simple interventions, such as HBV vaccination, education and providing containers for sharp instruments. Specific guidelines, similar to the American Occupational Safety and Health Administration (OSHA) regulations that have lowered by up to 88% of needle stabbing incidents, should be introduced by the European Union (EU) and other countries. The results of a review of reports leads to the following conclusions: 1) elaboration and implementation of new State regulations, especially in EU countries and in countries where such regulations do not exist; 2) the training of health care personnel should always be undertaken for new employees, and periodically for those already employed; 3) periodical control by appointed inspectors of knowledge of procedures for the prevention of injuries by sharp instruments among health care workers; 4) introducing and training in the use of equipment, which can prevent the sharp injuries; 5) an advanced monitoring system of sharp injuries sustained by worker should also be introduced. Successful implementation of these prevention measures will result in progress for public health and HCW’s health and safety.

10

Protective antigen domain 4 of Bacillus anthracis as a candidate for use as vaccine for anthrax

61%

Zakowska D. , Graniak G. , Rutyna P. , Naylor K. , Glowacka P. , Niemcewicz M.

Annals of Agricultural and Environmental Medicine

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2019

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tom 26

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nr 3

11

Microarrays - new possibilities for detecting biological factors hazardous for humans and animals, and for use in environmental protection

61%

Mirski T. , Bartoszcze M. , Bielawska-Drozd A. , Gryko R. , Kocik J. , Niemcewicz M. , Chomiczewski K.

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2016

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tom 23

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nr 1

12

Identyfikacja Francisella tularensis techniką real - time PCR z wykorzystaniem sond hybrydyzujących zaprojektowanych dla fragmentów sekwencji genów fopA i tul4

61%

Bielawska-Drozd A. , Niemcewicz M. , Gawel J. , Bartoszcze M. , Graniak G. , Joniec J. , Kolodziej M.

Medycyna Doświadczalna i Mikrobiologia

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2010

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tom 62

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nr 4

PL

Badano przydatność zaprojektowanych starterów FopA F/R, Tul4 F/R i sond hybrydyzujących FopA S1/S2, Tul4 S1/S2 dla genów fopA i tul4 do wykrywania F. tularensis. W badaniach, w których użyto 50 szczepów F. tularensis uzyskano wyniki dodatnie. Swoistość reakcji wykazano badając szczepy bakterii non-Francisella tularensis. Przy użyciu starterów FopA F/R i sond hybrydyzujących FopA S1/S2 zaprojektowanych dla genów fopA dodatnie wyniki amplifikacji uzyskano ze wszystkimi badanymi szczepami F. tularensis. Identyczne wyniki otrzymano w reakcji real - time PCR z zastosowaniem starterów Tul4 F/R i sond hybrydyzujących Tul4 S1/ S2 zaprojektowanych dla genu tul4. Swoiste produkty reakcji amplifikacji pojawiły się między 16 a 18 cyklem reakcji. Badania z użyciem starterów i sond hybrydyzujących zaprojektowanych dla genu fopA wykazały, że charakterystyczna temperatura topnienia produktów wynosiła 61°C, a dla genu tul4 60°C. Przy użyciu starterów Tul4 F/R i sond hybrydyzujących Tul4 S1/ S2 czułość oznaczeń wynosiła 10 fg/µl, a przy użyciu starterów FopA F/R i sond hybrydyzujących FopA S1/S2 1 fg/µl.

EN

Tularemia is highly infectious and fatal zoonotic disease caused by Gram negative bacteria Francisella tularensis. The necessity to undergo medical treatment in early phase of illness in humans and possibility of making use of bacterial aerosol by terrorists in an attack create an urgent need to implement a rapid and effective method which enables to identify the agent. In our study two primers FopA F/R and hybridization probes Fop A S1/S2 designed from fop A gene sequence, were tested for their potential applicability to identify F. tularensis. In this research 50 strains of F. tularensis were used and the test gave positive results. Reaction specificity was confirmed by using of non - Francisella tularensis bacterial species. The results obtained in the real-time PCR reaction with primers Tul4 F/R and hybridization probes Tul4 S1/S2, designed from tul4 gene, were comparable to the results from previous experiment with fopA - primers set. Investigation of fopA and tul4 primers and hybridization probes properties revealed characteristic Tm (melting temperature) value of the products - 61 °С and 60°C, respectively. Detection sensitivity was remarkably higher when fopA primers set was used 1 fg/µl, and for tul4 primers set, minimal detectable concentration is 10 fg/µl.

13

The role of Chromium III in the organism and its possible use in diabetes and obesity treatment

61%

Lewicki S. , Zdanowski R. , Krzyzowska M. , Lewicka A. , Debski B. , Niemcewicz M. , Goniewicz M.

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nr 2

14

Review of methods used for identification of biothreat agents in environmental protection and human health aspects

51%

Mirski T. , Bartoszcze M. , Bielawska-Drozd A. , Cieslik P. , Michalski A. J. , Niemcewicz M. , Kocik J. , Chomiczewski K.

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nr 2

15

Bacillus anthracis infections – new possibilities of treatment

51%

Zakowska D. , Bartoszcze M. , Niemcewicz M. , Bielawska-Drozd A. , Knap J. , Cieslik P. , Chomiczewski K. , Kocik J.

Annals of Agricultural and Environmental Medicine

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2015

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tom 22

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nr 2

16

Effect of isopropyl methylphosphonofluoridate (IMPF) poisoning on selected immunological parameters of angiogenesis

51%

Zdanowski R. , Krzyzowska M. , Lewicki S. , Rutkowski P. , Lesniak M. , Saracyn M. , Niemcewicz M. , Michalski A. , Kowalczyk M.

Annals of Agricultural and Environmental Medicine

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2014

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tom 21

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nr 4

17

A new locality of the Haemaphysalis concinna tick (Koch, 1844) in Poland and its role as a potential vector of infectious diseases

51%

Zieba P. , Nowakiewicz A. , Michalski A. , Wlizlo-Skowronek B. , Gawel J. , Niemcewicz M. , Gnat S. , Lagowski D.

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2019

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tom 65

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nr 3

EN

The Haemaphysalis concinna tick is a rare species in Poland. To date, it was found only once a few decades ago. During tick collection for epidemiological studies, a stable population of this arachnid was found in a military training area near Nowa Dęba. This report is particularly important, given the role of Haemaphysalis concinna in the spread of dangerous vector-borne diseases.

18

Analysis of the efficiency of post-electrphoretic protein staining using colloidal coomassie blue G-250

51%

Dratwa-Chalupnik A. , Lepczynski A. , Ozgo M. , Herosimczyk A. , Michalek K. , Niemcewicz M. , Palyszka A. , Skrzypczak W.

Acta Scientiarum Polonorum. Zootechnica

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2015

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tom 14

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nr 2

19

Acute respiratory distress syndrome (ARDS) in the course of influenza A/H1N1v infection – genetic aspects

41%

Niemcewicz M. , Pajak B. , Michalski A. , Kocik J. , Kolodziej M. , Joniec J. , Graniak G. , Gawel J. , Marciniak-Niemcewicz A. , Kucharczyk K. , Prystupa A. , Witczak A. , Lasocki K. , Naylor K. , Goniewicz M.

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2013

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tom 20

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nr 4

20

Severe Influenza Outbreak in Western Ukraine in 2009 – a molecular-epidemiological study

41%

Kocik J. , Niemcewicz M. , Johns M. , Jerke K. , Michalski A. , Bielecka A. , Lasocki K. , Gawel J. , Bielawska-Drozd A. , Joniec J. , Kolodziej M. , Graniak G. , Goniewicz M. , Kubiak L.

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2013

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tom 20

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nr 3

EN

Introduction: In the autumn of 2009 the authors participated in a humanitarian operation in Western Ukraine by undertaking an epidemiological investigation of an influenza-like-illness (ILI) in the L’viv Oblast region. Mobile biological survey teams took samples from civilian patients with severe acute respiratory distress syndrome, rapid transportation of the samples, and their molecular analysis in Poland to provide accurate results. Objective: The aim of the study was the molecular and epidemiological analysis of the biological samples collected. Material and Methods: Real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR), multiplex PCR techniques, traditional Sanger Sequencing and classical viral culture methods were used. Results: Among the 124 influenza-like illness cases, ~50% (58) were positive for influenza A virus in WHO-CDC molecular assay, including subtyping. The specimens were further analyzed to confirm results and determine the genetic sequence. Phylogenetically, the nucleotide similarity of both the Ukraine specimens and reference A/California/7/2009 (pH1N1) was 99.2–99.3%. Oseltamivir resistance was not registered. HA1 region characterization showed an overall protein identity of 98.5–99.4%. Conclusions: An unexpected high contribution of influenza A was confirmed among ILI patients, as well as a very limited number of other detected viruses, indicate that the 2009 epidemic in western Ukraine was strongly related to novel influenza A/H1N1. The importance of swift sharing of information and reference laboratories networking in surveillance, as well as serving governments and international agencies in pursuing adequate actions, should be stressed.