This study investigated cross-reactivity between allergens of Cupressaceae and Fagaceae pollen. Human IgE raised against Cupressus arizonica pollen allergen was used to demonstrate the presence of related allergens on ultrathin sections of Fagaceae pollen (Quercus ilex, Castanea sativa, Fagus sylvatica). Tissue localization of the cross-reactive allergen was investigated by immunogold electron microscopy. TEM observations showed that IgE raised against C. arizonica allergen recognizes epitopes on Fagaceae pollen. The cross-reactive allergens can be found on the wall and in the cytoplasm.
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