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2 Acta Physiologiae Plantarum

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Effect of edible coatings on ‘Misty’ blueberry (Vaccinium corymbosum) fruits stored at low temperature

100%

Totad M. G. , Sharma R. R. , Sethi S. , Verma M. K.

Acta Physiologiae Plantarum

2019

tom 41

nr 12

Blueberry fruits are modern remedy for different health complications, due to presence of different nutrients and antioxidants. Nutrient rich blueberry cannot to be stored for many days because of its poor shelf life of merely 2–4 days at ambient conditions. Hence, there is lack of strategy to extend their availability for longer time. To fill this gap, the use of edible coatings has come to the way to increase the freshness of fruits. Hence, we studied the effect of four edible coatings such as carboxy methyl cellulose (1%), xanthan gum (0.3%), guar gum (0.75%) and gum Arabic (10%) on the ‘Misty’ blueberry fruits stored at low-temperature condition (1 ± 1 °C and 85–90% RH). During storage, observations on different physical and functional attributes were recorded till 35th day of storage. Our results showed that all the coatings were effective in extension of shelf life of coated fruits but the CMC-coated ‘Misty blueberry fruits exhibited 44% lesser weight loss and maintained 22% higher firmness over non-coated fruits. Similarly, such fruits maintained ⁓ 40% higher level of ascorbic acid, 16% total phenolic content, 14% total anthocyanin content and 13% antioxidant activity. Based on these findings, it can be concluded that under low-temperature storage condition, CMC (1%) was the most effective coating as it increased the shelf life of ‘Misty’ blueberry up to 35 days of storage while maintaining quality.

Direct rhizogenesis, in vitro stolon proliferation and high-throughput regeneration of plantlets in Glycyrrhiza glabra

75%

Gupta S. , Pandotra P. , Gupta A. P. , Verma M. K. , Ahuja A. , Vishwakarma R. A.

Acta Physiologiae Plantarum

2013

tom 35

nr 09

Direct rhizogenesis from leaf explants and establishment of an in vitro stolon culture system and subsequent plant regeneration for Glycyrrhiza glabra have been described. MS liquid medium supplemented with 0.01 mg l-1 of NAA was most effective for stolon proliferation. Extensive proliferation of stolon and shoot regeneration was achieved on medium containing 3 % sucrose with 0.01 mg l-1 NAA. Stolons with nodes showing growth was transferred under light for plantlet regeneration in the same medium. This paper is the first report in G. glabra describing a complete regeneration procedure via in vitro stolon proliferation along with quantitative data for glycyrrhizin and genetic fidelity of plant regenerated in vitro there from. In vitro stolon proliferation described here would be an efficient way for regeneration of plants for functional genomics studies and better understanding of glycyrrhizin (GA) metabolism.