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EN
Semen was collected from Polish Halfbred stallions. Twenty individuals from 3 to 4 years of age were selected for the study. At least one ejaculate from each stallion was collected and assessed. Sperm morphology was evaluated using Papanicolaou stain, SpermBlue®, and a complex of eosin and gentian stain, whereas unstained slides were used as control samples. Morphometric measurements were performed on 100 randomly selected sperm heads in each ejaculate. The length, width, perimeter, and surface area of the sperm head were measured. The frequency of nuclear vacuoles was determined as well. Tygerberg's strict criteria, which most precisely characterise the sperm head, were used in the morphological evaluation of the sperm. The results obtained indicate that in the case of staining with SpermBlue® and with eosin+gentian complex, the morphometry of the sperm head seems to be most similar to that observed in unstained smears. It also seems that neither shrinking nor swelling is uniform in the various staining techniques (Papanicolaou stain). Moreover, it appears that in comparison with unstained sperm, all methods caused the width of the head to increase as a result of swelling.
EN
Routine semen examination is an important tool in diagnostics and fertility evaluation in humans and animals, both in vivo and in vitro. Among the most important parameters in semen evaluation are sperm motility and morphology. The aim of the study was a detailed morphological analysis of stallion sperm with particular attention to the parameters of the sperm head. The subject of the study was the sperm of Polish Half Bred stallions, isolated post mortem from the tail of the epididymis. The smears obtained were stained with silver nitrate Tygerberg’s criteria, which most precisely characterize the sperm head, and were used in the morphological evaluation of the sperm. The evaluation was expanded to include characterization of the tail, particularly the midpiece. The silver nitrate staining used in the present study enables staining of sperm structures which are not visible when other methods of smear preparation are used. In further research this method will be compared with others routinely used in laboratories in order to determine the extent to which the staining method affects sperm morphology and artefact formation.
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