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Compositional profiling and molecular docking studies of Eucalyptus polybrachtea essential oil against mucormycosis and aspergillosis

100%

Sharma A. D. , Kaur I. , Chauhan A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2023

tom 104

nr 3

Easy PCR screening of Pichia pastoris transformants

100%

Arora D. , Chauhan A. , Khanna N.

Cellular and Molecular Biology Letters

1998

tom 03

nr 1

A simple protocol for easy PCR screening of P. pastoris transformants is described. In short, the P. pastoris cells are lysed with very small amount of the enzyme Zymolase and the crude cell lysate is directly used in PCR. This protocol needs no tube transfer steps and also obliviates the requirement of freezing the samples at -80°C before PCR screening. Because of a single step screenig, both overall and actual hands on time are considerably reduced.

Adsorption behavior of cadmium and nickel from aqueous solution by Saraca indica leaf powder

88%

Srivastava M. M. , Chauhan A. , Kumari P. , Sharma P. , Srivastava S.

Archiwum Ochrony Środowiska

2005

tom Vol. 31, no. 3

59--70

The development of economic and efficient processes for the removal of toxic metals from water bodies has become a priority task for environmentalists. Biosorption processes arc tangible alternatives to traditional methodologies, particularly if low metal concentration, large volume and cost arc considered. The present communication reports the unexploited sorption properties of the Saraca indica leaf powder (S1LP) for the removal of Cd(ll) and Ni(II) from aqueous media. Sorption studies using standard practices were carried out in batch experiments as a function of biomass dosage, metal concentration, contact time, particle size and pH. Sorption studies result into the standardization of optimum conditions for the removal of Cd(II) - 92.60% and Ni(II) - 46.20% as follows: biomass dosage (4.0 g), metal concentration (Cd(II) 10 ug/cm3, Ni(II) 10 ug/cm3) and volume of the test solution (200 cm3) at pH 6.5 for Cd(II) and Ni(II). The present study explores for the first time, the efficacy of Saraca indica leaf powder as a novel and environment friendly possibility to remediate heavy metal contaminated water in a cost efficient manner.

Adsorption behavior of cadmium and nickel from aqueous solution by Saraca indica leaf powder

88%

Srivastava M. M. , Chauhan A. , Kumari P. , Sharma P. , Srivastava S.

nr 3

Plant growth-promoting traits of phosphate solubilizing bacteria isolated from Hippophae rhamnoides L. (Sea-buckthorn) growing in cold desert Trans-Himalayan Lahul and Spiti regions of India

75%

Kumar A. , Guleria S. , Mehta P. , Walia A. , Chauhan A. , Shirkot C. K.

Acta Physiologiae Plantarum

2015

tom 37

nr 03

One hundred and six P-solubilizing bacteria (PSB) from rhizosphere soil (RS) and root endosphere (ER) of Hippophae rhamnoides L.(Sea-buckthorn) of two locations viz. Lahaul and Spiti of Himachal Pradesh were isolated using culture-dependent procedures and were screened for various plant growth-promoting traits (PGPTs). Indole acetic acid produced was detected in 76.41 % isolates, siderophore synthesis in 43.39 % isolates and hydrogen cyanide in 19.4 % isolates. The percent growth inhibition against Fusarium oxysporum and Alternaria sp. was detected in 25.5 and 47.2 % isolates, respectively. Overall, 23.56 % of PSB isolates from RS and 16.85 % from ER showed none of the PGPTs tested. Among the PSB showing PGPTs, 36.58 % had single trait and 43.08 % had multiple traits showing two (29.68 %) and three (13.82 %) types of PGPTs. The Shannon–Weaver diversity index revealed that the proportion of PSB isolates possessing PGPTs was higher for RS than for the ER. Furthermore, the five best performing PSBs under in vitro conditions were selected for pot experiment to evaluate their efficiency for growth promotion of tomato seedlings under net house conditions. Among five isolates, Bacillus subtilis CKS1 exhibited significant increase in shoot length (13.82 %), root length (25.07 %), shoot dry weight (29.47 %) and root dry weight (33.33 %). Plant growth-promoting and antifungal activities of Bacillus subtilis CKS1 depict that it may be utilized as a potential biofertilizer for tomato.