Wyniki wyszukiwania - Biblioteka Nauki

1

Wlasciwosci acetylohydrolazy plytkowego czynnika aktywujacego [PAF-AH] z plazmy nasienia knura

100%

Kordan W.

Rozprawy i Monografie. Uniwersytet Warmińsko-Mazurski w Olsztynie

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2001

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tom 44

1-53

2

Wydzial Bioinzynierii Zwierzat Uniwersytetu Warmisko-Mazurskiego w Olsztynie

100%

Kordan W.

Przegląd Hodowlany

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2006

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tom 74

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nr 07

31-33

3

Mechanism of action of the sperm motility inhibiting factor [SMIF] from boar seminal plasma

100%

Kordan W.

Natural Sciences

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1999

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tom 02

4

Characteristics of selected seminal plasma proteins and their applications in the improvement of the reproductive processes in mammals

63%

Mogielnicka-Brzozowska M. , Kordan W.

Polish Journal of Veterinary Sciences

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2011

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tom 14

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nr 3

EN

Understanding the biochemical processes associated with ovum fertilization and knowledge about the structure and function of individual substances participating in these processes is crucial for the development of biotechnological methods to improve reproduction of animals and humans. Among many components of seminal plasma, proteins and peptides play a specific role in regulation of the fertilization process, particularly through their ability to bind various types of ligands such as polysaccharides, lipids and ions. Heparin-binding proteins regulate capacitation and acrosome reaction processes. Affinity of plasma proteins to mannans of the fallopian tube epithelium facilitates formation of spermatozoa reservoirs in the female reproductive tract. Ability to bind phosphorylcholine is one of the conditions for the coating of the seminal plasma proteins on the sperm membrane and also determines the formation of oligomeric forms of certain proteins. Zinc binding by seminal plasma proteins regulates sperm chromatin condensation state. It also affects motility of these cells and acrosome reaction. The interspecies analysis indicates significant structural and functional similarities, especially for the proteins with low molecular weight. Fertility associated proteins (FAPs) have been determined in the bull, stallion, boar, ram and dog. The contents of these proteins correlate with the indicators of the fertilizing abilities of sperm. In humans, several seminal plasma proteins were found which serve as diagnostic markers of spermatogenesis, seminiferous epithelium state, and azoospermia. To determine the semen ability for preservation, measurement of some seminal plasma protein content may also be used. Addition of specific plasma proteins to a spermatozoa solution undergoing the process of preservation may be used to retain the features of the cells responsible for efficient fertilization.

5

Syntetyczny plytkowy czynnik aktywujacy [PAF] jako skladnik rozcienczalnika dla nasienia knura

63%

Kordan W. , Strzezek J.

Medycyna Weterynaryjna

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2006

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tom 62

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nr 05

560-562

EN

The aim of this study was to investigate the effects of PAF addition on selected motility characteristics and plasmalemma integrity of boar spermatozoa following liquid storage in a boar semen extender, Kortowo-3 (K-3), supplemented with lipoprotein fractions extracted from hen egg yolk (LPFh) or lyophilized lipoprotein fractions extracted from ostrich egg yolk (LPFo), at 5°C and 16°C. Sperm motility was evaluated using a computer system (CASA). The determination of AspAT activity in sperm extracts as well as fluorescent analysis, with a fluorochrome, Hoechst 33258, were used to assess the plasmalemma integrity overlying the middle-piece and acrosomal regions of spermatozoa, respectively. It was confirmed that the addition of exogenous PAF to K-3 extender containing LPFh or LPFo had a beneficial effect on the sperm quality parameters during storage at 5°C or 16°C. This phenomenon was manifested by an increase in motility and survivability of spermatozoa. The use of LPFh or LPFo as a component of boar semen extender had a protective effect on the plasmalemma integrity overlying the middle-piece and acrosomal regions of PAF-treated spermatozoa.

6

Biochemiczne mechanizmy regulacji ruchu plemnikow ssakow

63%

Kordan W. , Strzezek J.

Postępy Biologii Komórki

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1997

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tom 24

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nr 4

543-560

7

Effects of decondensing agents on chromatin stability of boar spermatozoa - radioisotope study

63%

Strzezek J. , Kordan W.

Animal Science Papers and Reports

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2003

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tom 21

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nr 3

EN

Chromatin stability is an important determinant of semen quality, essential for spermatozoa maturation in epididymes and early embryogenesis. A radioisotope method based on the quantitative measurements of tritium-labelled actinomycin D (3H-AMD) incorporation into the spermatozoa nuclei was used to assess chromatin stabilization of boar spermatozoa incubated with physiological (reduced glutathione – GSH, heparin – H and bosine serum albumin – BSA) or non-physiological (dithiothreitol – DTT, disodium ethylenediaminetetra acetate – EDTA, 2-mercaptoethanol – ME and sodium dodecyl sulphate – SDS) decondensing agents.The effect of the composition of seminal plasma and the role of zinc ions in chromatin stability of spermatozoa were also studied. Pre-treatment of spermatozoa with GSH, H, DTT, ME or SDS resulted in an increase in the incorporation of 3H-AMD into the spermatozoa nuclei. In contrast, when sperm samples were treated with BSA or EDTA there was a reduction in the incorporation of 3H-AMD, what was attributed to hyperstabilization of chromatin. A presumed hyperstabilization was also observed when SDS+EDTA+H were used. On the other hand, an exceptionally strong action of decondensation of chromatin was induced by H+BSA. Increased incorporation of 3H-AMD into the spermatozoa nuclei was concomitant with low zinc and protein content in the seminal plasma of boars following depletion test (DT), suggesting disturbances in chromatin stability. The presented radioisotope method based on the application of 3H-AMD is a simple and reliable assay that can be used to monitor the chromatin status of boar spermatozoa.

PL

Stabilność chromatyny plemników jest istotnym wyznacznikiem jakości nasienia, bowiem determinuje przebieg procesu dojrzewania plemników w najądrzach oraz wczesny rozwój zarodkowy.W badaniach zastosowano radioizotopową metodę pomiaru wiązania 3H-aktynomycyny D (3H-AMD) przez jądra plemników inkubowanych z czynnikami dekondensującymi chromatynę: fizjologicznymi (zredukowany glutation – GSH, heparyna – H, albumina surowicy bydlęcej – BSA) i niefizjologicznymi (ditiotreitol –DTT, wersenian dwusodowy – EDTA, merkaptoetanol – ME, laurylosiarczan sodowy – SDS). Badano wpływ składu plazmy nasienia na stabilność chromatyny plemników, ze szczególnym uwzględnieniem funkcji jonów Zn2+. W przypadku zastosowania GSH, H, DTT, ME i SDS stwierdzono wzrost wiązania 3H-AMD przez chromatynę plemników. Po inkubacji plemników z BSA lub EDTA obserwowano spadek wiązania. Pod wpływem grupy czynników dekondensujących w mieszaninie inkubacyjnej: SDS-EDTA-H,w nieobecności DTT (specyficznie rozszczepiającego mostki -S-S-) obserwowano spadek wiązania 3HAMD, co zinterpretowano jako hyperstabilizację chromatyny. Silne działanie dekondensujące chromatynę przez kompleks czynników fizjologicznych BSA-heparyna, uznano za skutek ich działania jako układu ligandów wiążących Zn2+, co sprzyjało destabilizacji wiązań S-Zn-S.Dziesięciodniowe próby opróżniania knurów, czemu towarzyszyło stopniowe obniżanie produkcji plemników i zdolności sekrecyjnej dodatkowych gruczołów płciowych, były sprzężone ze spadkiem wiązania 3H-AMD. Wyraźne obniżenie zawartości białka całkowitego i koncentracji jonów Zn2+ w plazmie niasienia obserwowane podczas prób opróżniania podkreśla udział tych komponentów w stabilizacji chromatyny plemników knura.Zastosowana metoda radioizotopowa z użyciem 3H-AMD może być wykorzystana w biochemicznej ocenie stanu chromatyny plemników.

8

The influence of the sperm motility inhibitin factor [SMIF] from boar seminal plasma on the ATP and cAMP contents in spermatozoa

63%

Kordan W. , Strzezek J.

|

nr 6-7

9

Effect of platelet-activating factor on motility parameters and plasmalemma integrity of boar spermatozoa

63%

Kordan W. , Strzezek J.

Animal Science Papers and Reports

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2002

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tom 20

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nr 1

10

Supplementation of different concentrations of Orvus Es Paste (OEP) to ostrich egg yolk lipoprotein extender improves post-thaw boar semen quality

51%

Fraser L. , Jasiewicz E. , Kordan W.

Polish Journal of Veterinary Sciences

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2014

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tom 17

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nr 2

EN

This study aimed to compare post-thaw quality of boar semen following freezing in an ostrich egg yolk lipoprotein (LPFo) extender supplemented with 0%, 0.25% and 0.50% Orvus Es Paste (OEP). Sperm assessments included total motility (TMOT), mitochondrial function (MF), plasma membrane integrity (PMI) and acrosome integrity (normal apical ridge, NAR). Considerable variations among boars and OEP treatments had a significant effect (P < 0.001) on post-thaw sperm characteristics. It was observed that post-thaw sperm characteristics were significantly compromised in semen samples frozen in the absence of OEP. By contrast, lactose-LPFo-glycerol extender supplemented with either 0.25% OEP or 0.50% OEP markedly enhanced post-thaw sperm characteristics. In all boars, there were no marked differences in post-thaw sperm TMOT between the freezing extenders supplemented with 0.25% and 0.50% OEP. However, a decline in the percentage of post-thaw motile spermatozoa was more pronounced in the extender supplemented with 0.50% OEP following a 120-min incubation period. Furthermore, the proportions of frozen-thawed spermatozoa with MF, PMI and NAR acrosomes varied significantly among the boars in the OEP-supplemented extenders. The findings of this study indicate that different OEP concentrations, in the presence of ostrich egg yolk lipoproteins, could have varying effects on post-thaw sperm survival.

11

Effect of platelet-activating factor on motility, plasmalemma integrity, the process of capacitation and acrosome reaction of fresh and cryopreserved boar spermatozoa

51%

Kordan W. , Lecewicz M. , Tobolski J.

Polish Journal of Veterinary Sciences

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2009

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tom 12

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nr 2

175-181

EN

The aim of the present study was to establish the effects of platelet-activating factor (PAF) on selected movement parameters, plasmalemma integrity, capacitation process and acrosome reaction in cryopreserved boar spermatozoa. A positive effect of PAF addition to cryopreserved semen on sperm motility was demonstrated, particularly with the application of phospholipid concentration of 1 x 10-6M-1 x 10-5M. A moderate induction of plasmalemma damage of cryopreserved spermatozoa was observed when PAF was used at a low concentration (1 x 10-8M-1 x 10-7M). The rate at which PAF induced the process of capacitation was inversely proportional to its concentration in the sample (the highest for the concentration of 1 x 10-8M, and the lowest at 1 x 10-5M). In turn, the strongest induction of acrosome reaction of spermatozoa was observed in samples with the addition of PAF at a concentration of 1 x 10-7M. The results obtained suggest that the application of PAF supplement to post-thawed boar semen can be used as a laboratory test of the ability of spermatozoa to induce the acrosome reaction.

12

Effect of dialysis of dog semen on sperm characteristics and some biochemical components of seminal plasma

51%

Strzezek R. , Koziorowska-Gilun M. , Kielczewski K. , Kordan W.

Polish Journal of Veterinary Sciences

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2015

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tom 18

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nr 2

EN

The aim of this study was to investigate the effect of dog semen dialysis on sperm characteristics and some biochemical components of seminal plasma. Whole ejaculates were dialyzed against Triscitrate-fructose extender for a 5 h period at room temperature (using semi-permeable cellulose tubing of 12-14 kDa molecular weight cut-off). It has been demonstrated that the long-term dialysis of dog semen causes a significant decrease in sperm quality parameters and disrupts the biochemical properties of seminal plasma. This procedure requires further improvement.

13

The ability of zinc-binding proteins from boar seminal plasma to bind heparin and phosphorylcholine

51%

Mogielnicka-Brzozowska M. , Strzezek J. , Dziekonska A. , Kordan W.

Animal Science Papers and Reports

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2014

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tom 32

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nr 4

EN

Zinc-binding proteins (ZnBPs) isolated from boar seminal plasma exert multiple effects on boar spermatozoa. The aim of this study was to determine whether the isolated zinc-binding proteins were capable of binding additional ligands. The affinity chromatography method was used for the first time to demonstrate that boar ZnBPs show affinity for both heparin (hep) and phosphorylcholine (pch). ZnBPs+hep accounted for approximately 20%, and ZnBPs+pch – for approximately 45% of total ZnBPs. Predominant polypeptides with molecular mass form 6.5 to 14 kDa were observed in both groups (ZnBPs+hep, ZnBPs+pch). The analyzed peptides’ low molecular mass and their ability to bind zinc ions as well as heparin and phosphorylcholine suggest that they belong to the family of multifunctional boar spermadhesins which perform their functions in the fertilization process by binding more than one ligand. The results indicate that the ZnBPs of boar seminal plasma may be involved in the processes associated with oocyte fertilization not only by zinc ions binding but also through heparin and phosphorylcholine.

14

Application of mass spectrometry [MS] in the structural analysis od selected polypeptides of boar seminal plasma obtained after two-dimensional electrophoresis [2-D PAGE]

51%

Kordan W. , Malinowska A. , Mogielnicka M. , Lecewicz M.

Animal Science Papers and Reports

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2009

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tom 27

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nr 1

41-50

EN

Two-dimensional electrophoresis (2-D PAGE) led to identification in the polypeptide maps of boar seminal plasma of four conserved polypeptides with identical molecular weight of 24 kDA, and different ranges of isoelectric point (pI): (1) 7.4-7.7, (2) 8.1-8.4, (3) 8.5-8.8 and (4) 9.2-9.4. In the current study the molecular structures of these polypeptides were analysed, for the first time, by mass spectrometry (LC – MS/MS). Computerized mass spectrometry analysis of the peptides obtained after trypsin-digestion of the polypeptides demonstrated their similarity to the family of spermadhesins (crystal structure of two members of the spermadhesin family), especially to epididymal spermadhesin AWN-1. In addition, homology was found of peptides 3 and 4 with a lysozyme C precursor (1,4-beta-N-acetylmuramidase C). The results presented might indicate the participation of the analysed polypeptides in the processes accompanying fertilization.

PL

Stosując metodę elektroforezy dwukierunkowej (2-D PAGE) zidentyfikowano w składzie map polipeptydowych plazmy nasienia knura cztery zakonserwowane polipeptydy o identycznej masie cząsteczkowej 24 kDa i następujących zakresach punktu izoelektrycznego: (1) 7,4-7,7; (2) 8,1-8,4;(3) 8,5-8,8 i (4) 9,2-9,4. W analizie ich struktury molekularnej zastosowano spektroskopię mas (LC – MS/MS). W prezentowanych badaniach, zastosowana po raz pierwszy komputerowa analiza widma masowego peptydów uzyskanych po trawieniu trypsyną wszystkich badanych polipeptydów, wykazała ich podobieństwo do rodziny spermadhezyn (cristal structure of two members of the spermadhesin family),zwłaszcza do spermadhezyny najądrzowej – AWN-1. Dodatkowo wykazano homologię polipeptydów 3 i 4 z prekursorem lizozymu C (1,4-beta-N-acetylmuramidase C). Prezentowane wyniki sugerować mogą udział analizowanych polipeptydów w procesach towarzyszących zapłodnieniu.

15

Expression of hypoxia-inducible factor-1alfa and association with Cu/Zn superoxide dismutase in the reproductive tract tissues of adult male roe deer (Capreolus capreolus) during the reproduction season

51%

Koziorowska-Gilun M. , Gilun P. , Koziorowski M. , Kordan W.

Annals of Animal Science

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2017

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tom 17

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nr 4

16

Effect of ostrich egg lipoproteins and hen egg yolk on the quality of dog sperm during liquid storage at 5°C

51%

Dziekonska A. , Behrendt D. , Strzezek R. , Kordan W.

Annals of Animal Science

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2018

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tom 18

|

nr 1

17

Analysis of proapoptotic changes in boar spermatozoa stored at 16 C in different short-term semen extenders

51%

Wysocki P. , Lyjak A. , Kordan W.

Bulletin of the Veterinary Institute in Puławy

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2013

|

tom 57

|

nr 3

EN

The aim of this study was to evaluate the effect of boar semen storage in different short-term extenders (BTS, Kortowo-3, and M III) on the percentage of spermatozoa showing proapoptotic and necrotic changes. For the first time in this study, Annexin V isolated from swine placenta has been used to determine proapoptotic changes in stored boar spermatozoa. Die changes were determined using the IN Cell Analyzer 2000. A gradual decrease in motility was observed on successive days of storage. Spermatozoa incubated in the BTS extender were characterised by the highest average motility, which reached 75% on the 1st d and 39% on day 5. Motility of spermatozoa stored in BTS was significantly higher than those stored in Kortowo-3 and M III extenders after 5 d of storage. Diluted semen contained 1.5% to 2.8% spermatozoa with proapoptotic changes. The discussed process was intensified on the 3rd d of storage when the percentage of apoptotic spermatozoa was determined at 8.3% to 14.6%, and the content of dead spermatozoa exceeded 25%. The analysed extenders differed insignificantly in their ability to protect semen against proapoptotic changes during storage. From the methodological point of view, Hoechst 33258 could be used additionally to stain sperm cells regardless of their status.

18

The effect of two packaging systems on the post-thaw characteristics of canine sperm

51%

Strzeżek R. , Polakiewicz P. , Kordan W.

Polish Journal of Veterinary Sciences

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2015

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tom 18

|

nr 2

EN

The aim of this study was to compare the effect of different packaging systems on some parameters of cryopreserved canine spermatozoa. The experimental material consisted of the sperm-rich fractions of ejaculates collected from four Beagle dogs. Semen samples for cryopreservation were stored in 0.25 ml plastic straws and two aluminum tubes with a total volume of 5.0 ml. Semen was frozen in static nitrogen vapor for 10 minutes (0.25 ml straws) or 15 and 20 minutes (aluminum tubes). Post-thaw assessments involved the determination of sperm motility parameters using a computer assisted sperm analyzer (CASA), sperm plasma membrane integrity (SPMI), mitochondrial membrane potential (MMP) and acrosome integrity (normal apical ridge, NAR). Regardless of the packaging system applied, no significant differences in total sperm motility (TMOT) or selected kinematic parameters were observed after freezing-thawing. However, spermatozoa frozen in 0.25 mL straws were characterized by improved functionality, in particular mitochondrial function, after thawing. The results indicate that large quantities of canine semen can be frozen in aluminum tubes. Further studies are required, however, to evaluate different freezing and thawing rates of aluminum tubes.

19

Single nucleotide polymorphism within arylsulfatase D gene (ARSD) is associated with selected kinematic parameters of sperm motility in Holstein-Friesian bulls

51%

Hering D. , Lecewicz M. , Kordan W. , Kaminski S.

Polish Journal of Veterinary Sciences

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2014

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tom 17

|

nr 3

EN

The aim of the study was to find out whether the single nucleotide polymorphism (SNP) within arylsulfatase D (ARSD) gene is associated with kinematic parameters of sperm motility in Holstein- Friesian bulls. 367 Holstein-Friesian bulls kept in one AI center were included in the study. Point mutation C/T at position 139037255 on chromosome X (rs42207167) was identified by PCR-RFLP method (Pflm I). Significant associations were found between ARSD genotypes and CASA-derived sperm motility parameters: average TM (Total Motility), average VSL (Straight Velocity), average VCL (Curvilinear Velocity) and for fraction of sperms showing progressive motility (a) of sperms (VSLa, VCLa and BCFa -Beat Cross Frequency). Most significant differences were observed between alternative homozygotes (CC vs TT). Our results suggest new role of arylsulfatase D gene as being involved in sperm motility.

20

Effect of extender supplementation with low-molecular-weight antioxidants on selected quality parameters of cryopreserved canine spermatozoa

51%

Lecewicz M. , Strzezek R. , Kordan W. , Majewska A.

Journal of Veterinary Research

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2018

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tom 62

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nr 2