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An abnormal phase removing method in phase measuring profilometry is proposed. In the five equal shifting phase steps algorithm, the shifting phase might be extracted from the deformed patterns captured by CCD camera. But there may be some errors introduced by a digital fringe projector and CCD camera in these deformed patterns. The impurity of the deformed patterns may lead to four classes of abnormalities when extracting the shifting phase. These abnormalities may cause the wrong shifting phase extraction by which the reconstructed object might be misshapen or anamorphic, or even in failure. By this proposed method, the above abnormalities can be removed, and the shifting phase can be auto-extracted precisely from the impure deformed patterns without knowing its value. Experimental results verify the feasibility and effectiveness of the proposed method.
EN
We investigated the response of Mn-hyperaccumulator Phytolacca americana L. to manganese excess as well as the relationships between lignin deposition in the plant’s leaves, peroxidase and laccase activities in the leaf apoplast, and Mn toxicity. The exceptionally high tolerance of P. americana to Mn, both in solution and in tissue, was confirmed. No visible brown spot was observed in the leaves of plants treated with B10,000 µM Mn for 10 days. Mn treatment significantly increased lignin content and laccase activity in the apoplastic washing fluid (AWF) of P. americana leaves. In contrast, an increase in the Mn supply was paralleled by a significant decrease in the concentration of total phenolic compounds (TPCs) and in water-soluble guaiacol peroxidase (SPOD) activity in leaf AWF. This result suggested that an increase in lignin deposition decreased the concentration of apoplastic TPCs that are available to generate potentially toxic intermediates by acting as peroxidase substrates. Thus, data of the present study indicate that lignin formation by laccase activities reduces Mn toxicity and increases Mn tolerance of P. americana by depressing SPOD-mediated formation of toxic intermediates from TPCs.
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