Wyniki wyszukiwania - Biblioteka Nauki

1

Pollination biology of Hemiboea ovalifolia (Gesneriaceae), an endangered herb from Guangxi, China

100%

Pu G. , Pan Y. , Wei C. , Tang S.

Polish Journal of Environmental Studies

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2019

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tom 28

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nr 4

EN

Although information on the reproductive biology of the endangered plant family Gesneriaceae is well known, the pollination mechanisms of these plants in karst regions are poorly understood. This study demonstrated the flowering phenology, pollinators, and breeding system of Hemiboea ovalifolia in karst regions. Findings revealed that the anthesis of H. ovalifolia often occurred late, during sunset, or early morning, with duration of 2-4 days; there was a certain level of temporal overlap between pollen viability and stigma receptivity; the most effective pollinators were Bombus ignitus and Anthophora zonata. Controlled pollination indicated that these plants were pollen limited and exhibited late-acting inbreeding depression resulting from the seed sets; there were significant differences in fruit sets between open-pollination and self-pollination or cross pollination, and in seed sets between self-pollination and cross-pollination or open-pollination. Despite the co-existence of large numbers of fruit and seed sets, and vegetative propagation in H. ovalifolia, a failure in seedling survival, and long duration to establishing first-year seedlings in natural populations suggests that the species does not easily recover from damage.

2

Evaluation of discrete-time VSC on an inverted pendulum apparatus with additional dynamics

100%

Hara M. , Furuta K. , Pan Y. , Hoshino T.

Applied Mathematics and Computer Science

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1998

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tom Vol. 8, no 1

159-181

EN

Discrete-time Variable Structure Control (VSC) with sliding sector is experimentally evaluated on a new rotational-type inverted pendulum apparatus including an additional dynamics which is used to investigate the robustness of the controller. It is experimentally shown that the discrete-time VS controller makes the system robust stable with respect to parameter uncertainties and gives a quick response against external disturbances, which yields a good control performance without chattering.

3

Effects of global risks – nitrogen additions on growth and competitive relations among invasive and native congeneric species – Bidens frondosa

100%

Pan Y. , Tang S. , Wei C. , Li X.

Polish Journal of Ecology

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2016

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tom 64

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nr 4

EN

Human activity has greatly increased the amount of biologically available nitrogen entering the natural environment. Addition of N can affect growth of and competitive interactions between native and invasive plants, thus increasing or decreasing the risk of invasion by alien species. Bidens frondosa is an invasive weed native to North America that recently has begun to spread in China. The influence of soil nutrient content on B. frondosa invasion has not yet been reported. In a common garden experiment, we compared the growth and competitive effects between B. frondosa and its co-occurring native congeners — B. tripartita and B. biternata — under three N levels (0 g m⁻², 1 g m⁻², 5 g m⁻²) to assess whether increased levels of N modifies risk of B. frondosa invasion. Our results showed that while N additions increased both the growth and competitive advantage of B. frondosa as compared with that of the native congener species, results are particularly pronounced under high N levels. While growth responses to N addition varied little among invasive populations under high N levels, the competitive effect of B. frondosa did vary among populations and was significantly greater than that of the congeners. Anthropogenic N additions are likely to increase risks of B. frondosa invasions. Thus, management efforts should focus on the reduction of N input to ecosystems to mitigate invasions by B. frondosa.

4

Generation of a promising universal RNAi vector system to control plant pests

100%

Pan Y. , Dong Y. , Wang R. , Yang M.

Acta Physiologiae Plantarum

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2019

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tom 41

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nr 01

5

Rough Rule Extracting From Various Conditions: Incremental and Approximate Approaches for Inconsistent Data

100%

Liu Y. , Xu C. , Zhang Q. , Pan Y.

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2008

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tom Vol. 84, nr 3-4

403-427

EN

Rough rule extraction refers to the rule induction method by using rough set theory. Although rough set theory is a powerful mathematical tool in dealing with vagueness and uncertainty in data sets, it is lack of effective rule extracting approach under complex conditions. This paper proposes several algorithms to perform rough rule extraction from data sets with different properties. Firstly, in order to obtain uncertainty rules from inconsistent data, we introduce the concept of confidence factor into the rule extracting process. Then, an improved incremental rule extracting algorithm is proposed based on the analysis of the incremental data categories. Finally, above algorithms are further extended to perform approximate rule extraction from huge data sets. Preliminary experiment results are encouraging.

6

Using three-dimensional visualization to represent hydrological influance on wetland plants

100%

Pan Y. , Nakagoshi N. , Gong H.

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nr 2-4

317-329

7

Effects of Global Risks — Nitrogen Additions on Growth and Competitive Relations among Invasive and Native Congeneric Species — Bidens frondosa

100%

Pan Y. , Tang S. , Wei C. , Li X.

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2016

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tom Vol. 64, nr 4

443--452

EN

Human activity has greatly increased the amount of biologically available nitrogen entering the natural environment. Addition of N can affect growth of and competitive interactions between native and invasive plants, thus increasing or decreasing the risk of invasion by alien species. Bidens frondosa is an invasive weed native to North America that recently has begun to spread in China. The influence of soil nutrient content on B. frondosa invasion has not yet been reported. In a common garden experiment, we compared the growth and competitive effects between B. frondosa and its co-occurring native congeners — B. tripartita and B. biternata — under three N levels (0 g m-2, 1 g m-2, 5 g m-2) to assess whether increased levels of N modifies risk of B. frondosa invasion. Our results showed that while N additions increased both the growth and competitive advantage of B. frondosa as compared with that of the native congener species, results are particularly pronounced under high N levels. While growth responses to N addition varied little among invasive populations under high N levels, the competitive effect of B. frondosa did vary among populations and was significantly greater than that of the congeners. Anthropogenic N additions are likely to increase risks of B. frondosa invasions. Thus, management efforts should focus on the reduction of N input to ecosystems to mitigate invasions by B. frondosa.

8

Functional analysis of tomato LeEIL1 in an Arabidopsis ein2 mutant

88%

Pan Y. , Chen G. , Lu C. , Chen X. , Hu Z.

Acta Physiologiae Plantarum

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2011

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tom 33

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nr 2

EN

The ETHYLENE-INSENSITIVE3 (EIN3)/EIN3- Like (EIL) EIN3/EILs, novel nuclear proteins, are located at the downstream position of the ethylene signal transduction pathway. LeEIL1, which is expressed in fruit throughout ripening, is key transcription factor in the ethylene signaling pathway in tomato. To reveal its function, the LeEIL1 gene has been transformed into and expressed in the ein2 mutant of Arabidopsis. The expression levels of the transgene in the single copy line, LeEIL1-ein2-b, were higher than those in the multiple-copy line, LeEIL1-ein2-a. The ethylene-insensitive phenotype of the ein2 mutant plants has been partially recovered by expression of LeEIL1. The florescence of LeEIL1-ein2-a and LeEIL1- ein2-b exceeded that of the ein2 mutant but was still less than that of wild type of Arabidopsis. The expression of four pathology-related genes (AtPR3, 4, AtPDF1.2 and AtGST2) has been analyzed in LeEIL1 transgenic ein2 mutant plants. The expression of AtPR3 and AtPR4, which was reduced in the ein2 mutant, was enhanced in the two transgenic Arabidopsis plants. The expression of the AtPDF1.2 gene was unaffected in the two transgenic Arabidopsis lines, the ein2 mutant and wild-type Arabidopsis plants. In addition, the expression level of AtGST2 in transgenic Arabidopsis plants was lower even than that in ein2 mutant and wild-type Arabidopsis plants.

9

Human menstrual blood–derived stem cells promote the repair of impaired endometrial stromal cells by activating the p38 MAPK and AKT signaling pathways

88%

Zhu H. , Jiang Y. , Pan Y. , Shi L. , Zhang S.

Reproductive Biology

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2018

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tom 18

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nr 3

10

High - grade mutant OmpF induces decreased bacterial survival rate

75%

Zhao Z. , Liu T. , Zhang L. , Luo M. , Nie X. , Li Z. , Pan Y.

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2014

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tom 61

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nr 2

EN

OmpF plays very important roles in the influx of antibiotics and bacterial survival in the presence of antibiotics. However, high-grade mutant OmpF and its function in decreasing bacterial survival rate have not been reported to date. In the present study, we cloned a high-grade mutant OmpF (mOmpF) and sequence analysis suggested that over 45 percent of the DNA sequence was significantly mutated, leading to dramatic changes in over 55 percent of the amino acid sequence. mOmpF protein was successfully expressed. When grown in the presence of antibiotic, the bacterial survival rate decreased and the antibiotic inhibition zone became larger with the increase of the mOmpF. It was concluded that concentration of high-grade mutant mOmpF dramatically influenced the bacterial survival rate. The study presented here may provide insights into better understanding of the relationships between structure and function of OmpF.

11

Contributions of radiation interception and radiation-use efficiency to biomass decrease due to potassium starvation depend on potassium deficiency intensities

75%

Pan Y. , Lu Z. , Li X. , Cong R. , Ren T. , Lu J.

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nr 04

EN

Yield loss due to insufficient potassium fertilizer supply has been well documented; however, the information about the negative effect of potassium deficiency on crop yield caused by ecophysiological determinants is not enough. A field experiment with three K treatments (severe K deficiency treatment, K₁; moderate K deficiency treatment, K₂; and sufficient K supply treatment, K₃) was conducted to (1) assess the effects of potassium deficiency on green leaf area index (GLAI) reduction; (2) quantify the contributions of single leaf area, leaf senescence, and leaf appearance to GLAI reduction under potassium deficiency; (3) reveal the changes in the contributions of accumulated radiation interception (RIacc) and radiation-use efficiency (RUE) to above-ground biomass (AM) decrease of oilseed rape under different K supplies. GLAI was restrained due to potassium deficiency, with a reduction ranging from 10.6 to 45.4%. The reduced single leaf area and accelerated leaf senescence caused by potassium starvation accounted for 5.9–23.7% and 2.4–29.0% reduction in GLAI, but delayed leaf appearance rate contributed little. The RIacc during the seedling stage in the K₁, K₂, and K₃ treatments was 101.2, 110.7, and 120.0 MJ m⁻² , respectively, and the RUE in the K₁, K₂, and K₃ treatments was 1.03, 2.22, and 2.98 g MJ⁻¹ , respectively, which caused a 61.7% and 48.2% reduction of the final harvested AM in the K₁ and K₂ treatments compared with the K₃ treatment. When AM reduction was less than 24.8%, RIacc was the main determining factor; however, it transferred to RUE when biomass decreased more. In conclusion, GLAI decreased due to potassium starvation was mainly caused by the reduced single leaf area and accelerated leaf senescence, and the relative contribution of RIacc and RUE to AM decline was related to the degree of potassium deficiency.

12

Morphology and ontogenesis of a marine ciliate, Euplotes balteatus (Dujardin, 1841) Kahl, 1932 (Ciliophora, Euplotida) and definition of Euplotes wilberti nov. spec.

75%

Pan Y. , Li L. , Shao C. , Hu X. , Ma H. , Al-Rasheid K. A. S. , Warren A.

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tom 51

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nr 1

13

Potential Jurassic/Cretaceous boundary in northeastern China

63%

Sha J. , Chen S. , Cai H. , Jiang B. , Yao X. , Pan Y. , Wang J. , Zhu Y. , He C.

Volumina Jurassica

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2006

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tom Vol. 4, no. 1

269-270

EN

On the basis of the geological ranges of the Buchia and dinoflagellate cyst assemblages and their global correlation, two distinct biostratigraphical boundaries (event horizons) can be distinguished in the continu ous deposits spanning the Jurassic/Cretaceous boundary in the Dong'anzhen Formation of Dong'an, Raohe County and the Dongrong Formation of boreholes SB86-11 and SB79-1 of Suibin, Suibin County of eastern Heilongjiang, northeasteren China (Fig. 1.). Level 1. The base of the Buchia fischeriana - Buchia unschensis assemblage is characterized by the occurrence of Buchia (e.g. B. unschensis) with inversoid ontogenetic growth of the right valve, and the absence of the underlying B. russiensis, one of the index species of the Buchia russiensis - Buchia fischeriana assemblage, with obliquoid ontogenetic growth of the right valve (Fig. 1, column 2b). The top of the dinoflagellate cyst Amphorula delicate assemblage underlies, but it is near this boundary (Fig. 1, column 2a). This level basically corresponds to the currently accepted Tethyan version of the Jurassic/Cretaceous boundary and the working base of the Cretaceous as recommended by the International Commission on Stratigraphy (www.stratigraphy.org) (Fig. 1, column 1), and approximately corresponds to the boundary between the Boreal Middle-Upper Volgian substages (Fig. 1, column 3). This is because the base of the Buchia unschensis Zone or the base of the Buchia fischeriana - Buchia unschensis assemblage nearly coincides with that of the Boreal Craspedites exoticus Subzone or Craspedites okensis ammonite Zone, which in turn correspond closely to the base of Tethyan Lower Berriasian Berriasella jacobi ammonite Zone (Fig. 1, column 1), which is the index ammonite zone of the Tethyan or the international chronostratigraphic base of Berriasian, and thus the base of the Cretaceous (Fig. 1, column 1). Level 2. The base of the Buchia volgensis - Buchia cf. subokensis - Buchia cf. okensis - Buchia unschensis assemblage, is characterized by those easy-to-recognize large-sized Buchia with inversoid ontogenetic growth of the right valve and even of the left valve, and absence of Buchia fischeriana (Fig. 1, column 2b). The base of the Oligosphaeridium pulcherrimum dinoflagellate cyst assemblage probably corresponds approximately to the base of this Buchia assemblage (Fig. 1, column 2a). This level is very near the Boreal version of the Jurassic/Cretaceous boundary, which corresponds approximately to the base of the Tethyan middle part of Middle Berriasian. This is because the bases of both Buchia volgensis and Buchia cf. okensis in the assemblage of Buchia volgensis - Buchia cf. subokensis - Buchia cf. okensis - Buchia unschensis are closely coincident with the base of the upper Lower Berriasian Boreal Hectoroceras kochi Zone (Fig. 1, columns 2b, 3), and the Tethyan upper Berriasella privasensis Subzone of the Tirnovella occitanica Zone of the middle part Middle Berriasian, approximately corresponds to the middle of the Boreal Lower Berriasian Substage (Fig. 1, columns 1, 3).

14

Effective purification in constructed wetlands using strontium-doped TiO2 coated on porous ceramic filter media

63%

Tung T. X. , Xu D. , Zhang Y. , Zhou Q. , Wang X. , Pan Y. , Disong C. , Ting Z. , Wu Z.

Polish Journal of Environmental Studies

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2019

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tom 28

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nr 6

15

Cloning and expression of cold-inducible RNA binding protein in domestic yak (Bos grunniens)

63%

Yang K. , Zhang Q. , Wen Z. , Pan Y. , Yu S. , He J. , Yang X. , Liu P. , Cui Y.

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tom 75

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nr 4

EN

Cold-inducible RNA binding protein (CIRP) is over-expressed during cold and many other stresses, and could regulate the adaptation to hypothermia. In the present investigation, the objective was to determine the expression of CIRP in adult yak heart, liver, spleen, lung, kidney, brain, ovary, testis and skin by relative quantitative real time polymerase chain reaction (RT-PCR), Western blot and immunohistochemistry from mRNA and protein levels. The CIRP open reading frame encoding was cloned from the domestic yak brain. Results of RT-PCR and Western blot showed the high expression level of CIRP in the heart, kidney, brain, testis and skin, and the lower expression level of CIRP in the lung. Immunohistochemical staining showed CIRP was expressed in the nucleus of neuronal cells, spermatogonia, primary spermatocytes and epidermal cells, and in the cytoplasm of the residual tissues. These observations may provide new data to understand and further study the important role of CIRP protein in the plateau adaptation of the domestic yak on long-term evolution. (Folia Morphol 2016; 75, 4: 460–466)

16

Molecular cloning, expression and characterization of Bmserpin-2 gene from Bombyx mori

63%

Pan Y. , Xia H. , Lu P. , Chen K. , Yao Q. , Chen H. , Gao L. , He Y. , Wang L.

Acta Biochimica Polonica

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2009

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tom 56

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nr 4

671-677

EN

Serpins are a broadly distributed family of protease inhibitors. In this study, the gene encoding Bombyx mori serpin-2 (Bmserpin-2) was cloned and expressed in E. coli. The Bmserpin-2 cDNA contains a 1125 bp open reading frame (ORF). The deduced protein has 374 amino-acid residues, contains a conserved SERPIN domain and shares extensive homology with other invertebrate serpins. RT-PCR analysis showed that Bmserpin-2 was expressed in all developmental stages of B. mori larvae and various larval tissues. Subcellular localization analysis indicated that Bmserpin-2 protein was located in the cytoplasm. Interestingly, real-time quantitative PCR revealed that the expression of Bmserpin-2 in the midgut of susceptible B. mori strain 306 significantly increased at 72 hours post inoculation (hpi) when infected with BmNPV. However, there was no significant increase of the Bmserpin-2 expression in resistant strain NB infected with BmNPV. Thus, our data indicates that Bmserpin-2 may be involved in B. mori antiviral response.