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EN
In this paper are presented the results of measurements determiny tin concentrations in the muscles, livers, kidneys and lungs taken from 15 cows from the voivodeship of Białystok, where small levels of environmental pollution with heavy metals were recorded and from 15 cows of the Katowice region, where significant environmental pollution with these metals were noted. The averages of these concentrations ranged: 0.025-0.040 µg/g per sample and were not dependent on the kind of sample or region. Tin concentrations in the examined organs and muscles within determined limits were not toxicologically dangerous for consumers.
PL
Celem pracy było oznaczenie zawartości selenu w wybranych tkankach koni rzeźnych oraz określenie jej zmienności w zależności od wieku i płci zwierząt. Materiał do badań stanowiły trzy mięśnie oraz narządy wewnętrzne (nerki, płuca i wątroba) pochodzące ze 117 tusz koni rzeźnych zakwalifikowanych do drugiej klasy jakościowej. W doborze materiału doświadczalnego uwzględniono zwierzęta obu płci oraz dwie grupy wiekowe: konie młode (od 6 do 18 miesięcy) i stare (od 10 do 13 lat). Pobrane próbki po wstępnym przygotowaniu poddano mineralizacji. Zawartość selenu w uzyskanych mineralizatach oznaczono przy użyciu spektrometru absorpcji atomowej ze wzbudzeniem elektrotermicznym i deuterową korekcją tła Zeeman, Spektra 220Z firmy Varian. Wykazano statystycznie istotne (p ≤ 0,01) różnice zawartości selenu pomiędzy wszystkimi badanymi tkankami. Najwięcej selenu stwierdzono w nerkach (0,487 ppm), istotnie (p ≤ 0,01) mniejszą zawartość tego pierwiastka oznaczono w wątrobie (0,177 ppm), a jeszcze mniejszą – w płucach (0,062 ppm). Najmniej badanego pierwiastka było w tkance mięśniowej (0,039 ppm). Uwzględniając wiek koni, w nerkach i mięśniach zwierząt młodych wykazano statystycznie większą (p≤0,01) zawartość selenu. Płeć miała niewielki wpływ na zawartość selenu zarówno w narządach wewnętrznych, jak i w mięśniach. Istotne (p ≤ 0,01) różnice dotyczyły jedynie nerek. Z trzech badanych mięśni najwięcej selenu oznaczono w mięśniu nadgrzebieniowym (m. supraspinatus), a nieznacznie mniej, ale w wymiarze statystycznie istotnym (p ≤ 0,01), w mięśniach: najdłuższym (m. longissimus pars thoracis) i półbłoniastym (m. semimembranosus). Różnice te dotyczyły jedynie mięśni zwierząt młodych. W grupie tej stwierdzono także istotne (p ≤ 0,01) różnice pod względem zawartości selenu w zależności od rodzaju mięśnia i płci. Najmniej selenu zawierały mięśnie zwierząt powyżej 10. roku życia. W tej grupie zwierząt nie wykazano istotnych (p ≤ 0,01) różnic pomiędzy badanymi mięśniami, a także nie wykazano istotnego wpływu płci na zawartość selenu.
EN
The objective of the study was to determine the content of selenium in selected tissues of slaughter horses and to define its variability depending on the age and sex of the animals. The research material consisted of three muscles and internal organs (kidneys, lungs, and liver) from 117 carcasses of slaughter horses classified into the second quality class. The experimental material selected comprised animals of two sexes and of two age groups: a group of young horses (6 to 18 month old) and a group of old horses (aged between 10 and 13 years). The samples taken were initially prepared and, next, mineralized. The content of selenium in the mineralisates produced was determined using an atomic absorption spectrometer with inductively coupled electro-thermal and deuterium background correction (Zeeman, Varian Spectra 220Z). Significant differences in the contents of selenium were proved to exist among all the tissues tested. The highest content of selenium was found in the kidneys (0.487 ppm), a significantly lower content of that element was determined in the liver (0.177 ppm), and even lower in the lungs (0.062 ppm). The muscle tissue contained the lowest amount of selenium (0.039 ppm). When considering the age of the horses, a statistically significantly (p ≤ 0.01) higher amount of selenium was found in the kidneys and muscles of the young animals. The sex had a minor effect on the content of selenium both in the internal organs and in the muscles. The statistically significant (p ≤ 0.01) differences were found only in the kidneys.Of the three examined muscles, the highest levels of selenium were determined in the supraspinatus muscle, a bit lower but statistically significant (p ≤ 0.01) amounts in the longissimus and semimembranosus muscles. Those differences were found only in the muscles of the young animals. Furthermore, in that group, significant (p ≤ 0.01) differences were found in the content of selenium depending on the type of muscle and sex of the animals. The muscles of the animals aged more than 10 years contained the lowest level of selenium. In that group of animals, no significant (p ≤ 0.01) differences were found among the muscles analyzed; no significant impact of the sex on the content of selenium was found either.
EN
The objective of the studies was to determine the content of tin in dog and cat feeds conserved in metal packaging and available in retail trade in Poland in 1995. Tin concentration in home feeds ranged from 0.003 to 0.0269 mg/kg of fresh mass. The lowest level of this element was found in As feed, the highest in Dogland beef feed. Foreign fodder for dogs contained this metal at a concentration from 0.001 to 0.026 mg/kg. The lowest concentration of tin was found in Rokus feed with chicken and the highest - in Chappi. In the cat fodder, the lowest concentration of tin was noted in Whiskas cans, the highest in Felix feed. On the basis of these results and data from literature, moreover, assuming similar toxicity of tin for animals, it can be stated that the content of tin in the studied fodder within the determined limits is not toxicologically dangerous for its dog and cat consumers.
PL
Metodą ASA oznaczono stężenie ołowiu, kadmu, miedzi i cynku w 8 gatunkach warzyw, owocach agrestu i glebach z ogrodów działkowych Lublina - „Pionier" i „Podzamcze". Analiza statystyczna uzyskanych wyników uwzględniała odległość pobrania próbek od jezdni oraz gatunek warzyw.
EN
Lead, cadmium, copper and zinc contents in vegetables, fruits of gooseberries and in soil of Lublin gardening plots "Pionier" and "Podzamcze" both situated along heavy traffic streets, and "Pionier" additional to the close vicinity of automobile factory were determined by atomic absorption spectrophofometry. Statistical analysis of the results respected the streets or factory distances from the gardening plots, and the species of the vegetables. Levels of the determined elements in the most of the samples were lower than permitted by the Ministry of Health Regulation established in 1993. According to that Regulation a slight exceeding of zinc content in the red beetroots and in the overground parts of the leek were found. Lead content in the parsley root and in the dill was close to the tolerance limit. Both lead and cadmium of the soil of "Podzamcze" gardening plot significantly exceeded the levels considered as tolerable for unpolluted grounds, whereas the soil of "Pionier" contained lead dose to limit of tolerance level.
EN
Lead, cadmium, and mercury were estimated in the liver and kidneys of 30 silver foxes from one fur fox farm. The animals were divided into three groups according to their age and developing reproduction disorders. Cd and Pb were determined by GFAAS with Zeeman correction, whereas Hg by cold vapour technique. Serum levels for Hg and Cd were below minimum detection limit of the method, while Pb accounted x(n=30)=0.149±0.1145 µg mL⁻¹. The liver and kidneys contained: 0.378±0.2002 and 0.274±0.1006 µg g⁻¹ of Pb (wet weights); 0.253±0.2836 and 0.288±0.1656 µg g⁻¹ of Cd, and 0.257±0.3403 and 0.600±1.1112 µg g⁻¹ of Hg, respectively. Coefficients of variation, CV%(n=30)) from 11.3% to 304.9% were recorded. Pb content in the serum and liver increased with age; however, that trend was not observed in the case of Cd and Hg. The data within every groups were characterised by wide statistical dispersion; Hg level differed in three magnitude order. The means significantly differed from medians, the most for Hg in kidneys; x(n=10)=0.871±l .4952 µg g⁻¹ versus µ1/2=0.0220 µg g⁻¹ in group of one-year-old foxes. The highest contents found in the individual animals, Hg>3.4 µg g⁻¹ in the liver and kidneys or Cd>l µg g⁻¹ in the liver, were considered toxic for health status of the flock, reproductive disorders including.
EN
Serum, livers and kidneys of 30 silver foxes from one breeding farm were subjected to analysis of the four microelements contents. The samples derived from 3 groups of animals (An=10) selected according to age and developed reproduction disorders. Cu, Cr and Mn were determined by a graphite furnace AAS whereas Zn by the flame AAS methods. Serum levels of Zn were the least variable (×(n=30)=4.72 ± 2.313 μg ·mL-1) and the mean of Cu content was 0.26 ± 0.244, of Cr was 0.029 ± 0.032 and of Mn was 0.074 ± 0.085 μg ·mL-1. The livers and kidneys contained respectively: 159.9 ± 23.66 and 74.25 ± 14.44 μg g⁻¹ of Zn; 34.03 ± 12.43 and 13.66 ± 1.67 μg g⁻¹ of Cu as well as 6.28 ± 0.97 and 2.60 ± 0.33 μg g⁻¹ of Mn. The most variable was Cr level achieving 1.00 ± 1.06 and 1.43 ± 2.64 μg g⁻¹ (all results per gram of wet weights). The differences between means and medians within the age groups did not exceed 41%, however chromium was an exception, its values differed more than 2 times. High zinc level found both in the serum and the organs resulted from its concentration in feedstuff being 1040,5 μg g⁻¹, exceeding the recommended level for farming foxes. Over-supplementation of dietary Zn might suggest connection between subtoxic action of Zn or its interaction with Cu or Cr followed by subsequent effect on reproduction events. Whether nature of Zn effects derived from direct target action or from Zn – Cu or Zn – Cr interactions have to be solved experimentally. Some foxes contained copper and chromium in livers and kidneys at levels exceeded significantly those concidered as physiological ranges for mammals, whereas manganese was within these limits. Thus, manganese was considered to keep homeostasis status of this element in the examined foxes.
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