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1 Journal of Applied Genetics

1 1999

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The influence of various in vitro culture conditions on androgenetic embryo induction and plant regeneration from hexaploid triticale (x Triticosecale Wittm.)

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Ponitka A. , Slusarkiewicz-Jarzina A. , Wedzony M. , Marcinska I. , Wozny J. , Wozna J.

Journal of Applied Genetics

1999

tom 40

nr 3

165-174

The effect of sucrose and maltose in culture media PII, C17 and MN6 on androgenetic embryo formation was investigated in seven F2 triticale hybrid progenies. In all genotypes, the highest number of androgenetic embryos (89.8-320.6/100 anthers) was formed on medium C17 containing maltose. Also the highest number of green plants (6.17/100 embryos) was regenerated from embryos obtained on PII with sucrose. The effect of the physical environment (temperature, light) in the first week of embryo culture on the regeneration medium was tested. The highest rate of green plants per 100 embryos (2.5-11.8) was obtained from incubation at 22oC in the dark. Key words: androgenetic embryos, incubation temperature, media, plant regeneration, triticale.