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tom 19
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nr 03
262-264
EN
Siberian sturgeons were given intraperitoneally 100 mg/kg b.m. of oxytetracycline (OTC) and 24 h after the injection they were immersed in lysozyme dimmer solution (KLP-602) at 100 µg/L of water for 30 min. The effect of OTC, applied separately and in combination with lysozyme dimer, on hepatopancreas morphology has been examined. The results of microscopic and ultrastructural examinations of the organ indicated the existence of morphological lesions following the administration of OTC: congestions and extravasations, parenchymal and lipid-related degeneration, and focal necrosis with relatively frequent infiltrations of lymphoid cells. Ultrastructural examination revealed damage to the mitochondria in hepatocytes. KLP-602 was found to have a protective effect in these changes. Immersion of the fish in an aqueous solution of lysozyme dimer was shown to have an immunomodulating effect, reducing the intensity of morphological changes that resulted from the administration of OTC.
EN
Microscopic analyses investigated specimens of internal organs and muscles of 20 BUT-9 turkeys fed for 16 weeks with mixtures supplemented with fat of varying peroxide values: as high as 5, 50 100 and 150 mEq O2/kg. During the 4-week experiment period 2, 3, 4 and 5% fat (rapeseed oil and poultry fat at a proportion of 66:34 oxidized under controlled conditions) was added respectively to the feed. Supplementing the oxidized fat at PV 5 mEq O2/kg did not cause any morphological lesions in the internal organs of the turkey hens. However, an increase of up to 50 mEq O2/kg in the fat peroxide value of their diet resulted in retrogressive changes and disturbances in the blood supply to the liver and cross striated muscles. Fat at PV 100 mEq O2/kg resulted in a slight intensification of the above-mentioned changes mainly in these organs, however, fat oxidation of 150 mEq O2/kg led to a visible increase in the type and intensity of lesions as well as the areas in which they occurred (kidneys and alimentary tract). The observed morphological defects were lesions of a damaging and adaptive profile.
EN
The sense of taste plays an important role in people evaluating the sensory quality of food. Nowadays, an increasing demand is put on the profession of veterinary surgeon and especially a veterinary inspector. This is related to the condition of the senses, which are necessary for the proper performance of various control or research activities. The aim of the study was to evaluate the ability of taste determination by Polish veterinary inspectors and to determine the extent to which sex, age and smoking influence the results. The study was carried out on 246 veterinarians (136 females and 110 males), aged 27 to 60, who were divided into two age groups. The younger group (≤ 35 years) consisted of 86 women and 71 men, and the older one (> 35 years) consisted of 50 women and 39 men. The non-smokers and smokers accounted for 61.38% and 38.62% of the respondents respectively. The study was conducted before noon in a sensory laboratory conforming to the requirements of PN ISO 8589 (Sensory Analysis – General Guidance For The Design Of Test Rooms). A test to verify the ability to recognize and distinguish the tastes, according to PN ISO 3972 (Sensory analysis. Methodology. Method of investigating sensitivity of taste) was used. The veterinarians evaluated 10 tastes (aqueous solutions of 6 standard substances representing sweet, salty, sour, bitter, umami and metallic flavors as well as water) orally. The samples were slowly examined, taking about 15–20 ml of solution, which was then removed to the spitton. The test was performed twice: better results were analyzed for gender, age and smoking. Statistical analysis of the test results was performed using Statistica 10 PL program. The results obtained show that 48.53% of women and 31.82% of men examined recognized correctly more than half of the taste samples, with 13.2% of women correctly diagnosing ≥ 90% of the samples, while 1.47% did not recognize them at all. In men the values were 3.64% and 10.91% respectively. The proportion of samples correctly diagnosed by non-smokers (women and men together) was 21.98% higher than by smokers. The average number of correctly recognized taste samples in non-smoking women and men was significantly higher (p ≤ 0.05) than in smokers of the same sex. The highest mean number of correct recognitions (6.4 ± 2.3) was found in younger non-smoking women and the lowest (2.6 ± 2.4) in smoking men over 35 years of age. The results obtained in the younger age group were better than those from the older group. However the differences were statistically significant (p ≤ 0.05) only in non-smoking women. The best recognized tastes were: sweet, sour and salty. Clearly lower recognition was found in the case of umami taste and the lowest for bitter and metallic taste. Women were more likely than men to recognize taste categories, and the percentage of correct recognitions of salty, sour and umami tastes was significantly higher in women than in men (p ≤ 0.05).
EN
Paratuberculosis or Johne’s disease is a chronic granulomatous enteric disease afflicting cattle, sheep, goats and other ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). Calves are infected mainly via an oral route, especially from manure and environment contamination by infected adult cattle. It is generally assumed that calves rarely shed MAP bacteria and that calf-to-calf transmission is of minor importance. The aim of the study was to evaluate occurrence of faecal excretion of MAP by calves born to MAP-positive and MAP-negative dams in a low MAP prevalence herd. The study involved 66 dam-calf pairs. Samples of faeces were collected: for each cow once about 2-3 weeks to parturition, and for each calf 3 times at 2-5, 180-185 and 360-365 days of age. All samples were studied for the presence of MAP by culture or by PCR of IS900 insertion sequence MAP DNA. Faecal samples of the dams demonstrated 15/66 dams with at least one positive result. Analysis of MAP shedding prevalence in the faeces of calves originating from MAP-positive and MAP-negative dams shows that in 15 MAP-positive dams: 10 out of 15 calves (66.66%) at the age of 180-185 days and at the aged of 360-365 days 11 out of 15 calves (73.33%) were MAP-positive in faecal samples. In the case of MAP-negative dams: 7 out of 51 calves (13.72%) at the age of 180-185 days, and 8 out of 51 calves (15.68%) at the age of 360-365 days were MAP-positive. MAP-infection cases in calves coming from infected and healthy dams prove the occurrence of calf-to-calf transmission and that contact with infected calves increases the risk of MAP spreading among healthy calves.
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