Wyniki wyszukiwania - Biblioteka Nauki

1

Inwazje pasożytów - nieustanna wojna

100%

Zietek J.

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nr 03

2

Wektory chorób odzwierzęcych

63%

Zietek J. , Domagala M.

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nr 4

3

Szczepienia ochronne psów - teoria i praktyka

63%

Zietek J. , Adaszek L.

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nr 2

4

Borelioza. Problem czlowieka i zwierzat

63%

Zietek J. , Zietek E.

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nr 2

44-46

5

Burunduk - nowy pacjent klinik weterynaryjnych

63%

Zietek J. , Paczek A.

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nr 4

6

Przewlekłe zapalenie dróg oddechowych (CRD) u szczurów

63%

Zietek J. , Chrostek A.

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nr 05

7

Mieszane zakazenia na tle Babesia spp. i Ehrlichia spp. u psow

51%

Adaszek L. , Winiarczyk S. , Zietek J. , Kutrzuba J.

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tom 05

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nr 3

62

8

Istota choroby oraz aspekty praktyczne terapii kunikulozy (encefalitozoonozy) królików

51%

Zietek J. , Dziegiel B. , Adaszek L. , Winiarczyk S.

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nr 01

EN

Encephalitozoon cuniculi is an obligatory intracellular microsporidian parasite that can infect a wide range of mammals, including rodents, rabbits, horses, carnivores and humans. The main host for E. cuniculi is the rabbit, and infections usually have a sub-clinical course. Clinical symptoms observed in sick animals are those from the central nervous system and the urinary tract. The diagnosis and treatment of encephalitozoonosis in rabbits is difficult. Causal treatment involves the administration of the benzimidazole class of drugs, which demonstrate activity against protozoa. In symptomatic treatment, steroidal anti-inflammatory drugs may be administered to reduce the swelling of the brain. Flatulence is treated with formulations containing dimethicone or simethicone. The aim of this paper was to present the authors’ observations concerning the standards of rabbit encephalitozoonosis therapy.

9

ABC ziololecznictwa

51%

Zietek J. , Potyrala M. , Biegala P.

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nr 4

46-48

10

Struktura parwowirusa psów

51%

Adaszek L. , Zdanska D. , Zietek J. , Winiarczyk S.

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nr 12

11

Siara a zdrowie cieląt

51%

Zietek J. , Adaszek L. , Kalinowski M. , Koszykowska M.

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tom 05

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nr 2

12

Genetic variability among canine parvovirus strains currently circulating in Poland

51%

Wojcik A. , Zietek J. , Staniec M. , Winiarczyk S.

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nr 05

13

Możliwości i przyszłość szczepionek w aspekcie ciągłego rozwoju

51%

Adaszek L. , Gorna M. , Zietek J. , Winiarczyk S.

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nr 03

14

Hodowle in vitro pierwotniakow Babesia izolowanych od zwierzat

51%

Adaszek L. , Winiarczyk S. , Zietek J.

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tom 65

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nr 01

12-14

EN

Babesia organisms are hemoprotozoa isolated from people and animals. Their in vitro cultivation supplies information about the morphology and ultrastructure of these parasites. They make it possible to obtain the genetic material for diagnostic elaborations and for detections of phylogenetic relationships between various species of Babesia. Protozoa cultivated on red blood cells can be a source of parasite antigen or attenuated strains of protozoa which can be used for vaccinations. The cultures of Babesia species are kept in an erythrocytes host, taken from the animals from which Babesia is most frequently isolated, suspended in tissue culture media with the addition of sera, in microaerophil conditions at 37°C. The specific circumstances and factors that are needed to obtain an effective Babesia culture are very often the reason of the failure of these investigations.

15

Salmonelloza psów. Choroba groźna dla zwierząt i ich opiekunów

51%

Zietek J. , Adaszek L. , Winiarczyk S.

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nr 04

16

Method of dissecting edible snails of the genus Cornu

51%

Zietek J. , Ziomek M. , Wilczynska A.

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nr 10

EN

Snails of the genus Cornu are farm-raised as edible molluscs. Dissection is one of the basic diagnostic tests available in the breeding of these animals, used to determine the cause of death or disease, and to collect material for further laboratory tests. The aim of this article was to present a method for the dissection of snails for veterinary use based on the experience gained from 200 mollusc dissections, and to present a short description of the anatomical structure of snails. The method described is characterized by its speed and simplicity. The observations made during the dissection, as presented in the article provide valuable diagnostic information for veterinarians responsible for care on snail farms.

17

Kolisepticemia cielat. Przypadek kliniczny

51%

Zietek J. , Listos P. , Surma-Kurusiewicz K. , Adaszek L.

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nr 1

53-55

18

Choroby tropikalne psów i kotów. Wybrane jednostki kliniczne

51%

Zietek J. , Adaszek L. , Winiarczyk S. , Kutrzuba J.

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tom 05

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nr 5

19

Anaplazmoza trombocytarna - utajona choroba psów

45%

Adaszek L. , Bartnicki M. , Carbonero A. , Zietek J. , Winiarczyk S.

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nr 03

20

Porownanie klasycznej metody PCR oraz Real-Time Sybr-Green HRM PCR w rozpoznawaniu zakazen parwowirusowych u psow

45%

Adaszek L. , Twarog D. , Zietek J. , Wojciechowski M. , Winiarczyk S.

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tom 65

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nr 10

683-686

EN

The aim of this study was to compare a standard PCR and Sybr-Green HRM PCR in the diagnosis of canine parvoviral infections. A total of 22 feces samples were collected from dogs suspected of parvovirosis. The entire DNA for standard PCR and Real-Time PCR was isolated from the feces. In both methods this same pair of primers that allow the amplification of a fragment of VP 2 gene with a length of 1278 bp were used. The specificity of the obtained PCR products in the classical method were established based on the results of sequencing 8 out of 22 DNA probes and based on the comparison of their sequences with a CPV VP2 FJ 222823 sequence taken from the GeneBank. The specificity of Real-Time PCR products were established based on the analysis of their melting curve. In both standard and Real-Time PCR CPV DNA was detected in all 22 feces samples. The length of the obtained products was 1190 bp. To obtain a positive result in Real-Time PCR it was required to increase the number of the cycles from 30 to 60. The Ct values were between 43-53, and the analysis of the melting curve revealed that the Tm of Real-Time PCR products ranged between 80.5-85°C. Despite the results of this study indicating that both of these techniques are specific, sensitive, and repeated methods for detection of the CPV DNA, to shorten the time of Real-Time PCR the application of appropriate primers is required, which enables the amplification of shorter fragments of the DNA than those obtained in our study.