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High epidermal growth factor concentration associated with somatic cell count in milk of cows with subclinical mastitis

100%

Gokceoglu A. , Yarim F. , Gultiken N. , Yarim M.

tom 76

nr 06

The aim this study was to determine the milk epidermal growth factor (EGF) concentration in cows with subclinical mastitis and its relationship with the somatic cell count (SCC). The animal material of this study was composed of 40 lactating cows aged 3-6 years. Subclinical mastitis was diagnosed using the California Mastitis Test and SCC in milk. The study group consisted of 20 cows with SCC > 200.000 cells/ml, and the control group comprised 20 cows with SCC < 200.000 cells/ml. EGF concentration in milk was determined using a bovine-specific enzyme-linked immunosorbent assay (ELISA) kit. The mean EGF concentration was 6.08 ± 2.91 ng/ml in the study group and 2.85 ± 1.87 ng/ml in the control group (P < 0.001). The results also indicated a significant correlation between SCC and EGF concentration in the study group (r = 0.965, P < 0.01). The findings of this study suggest that a milk EGF assay together with SCC could be useful for diagnosing mastitis as well as for monitoring udder health.

Cytochrome P450 aromatase expression in canine nervous tissue: an immunohistochemical study

100%

Karahan S. , Yarim M. , Harada N.

2008

tom 11

nr 4

347-352

The enzyme cytochrome P450 aromatase is responsible for conversion of androgens to estrogens. Estrogens have been implicated in neurophysiology and neuropathology. The present study investigated the presence of aromatase immunoreactivity in the temporal, parietal and occipital cortices, olfactory bulb, cerebellum, and choroid plexus of the normal dog. Aromatase immunoreactivity was localized exclusively in neurons in the cortices and olfactory bulb. Immunoreactivity was also present in a small number of astrocytes in the substantia alba of the cerebellum. In the cortical regions, immunoreactive neurons, morphologically identified as pyramidal cells, were found throughout Layer II down to Layer VI, but not all pyramidal neurons were immunoreactive. In the olfactory bulb, immunoreactive neurons were mainly observed in mitral cells and inner granular cell layers. In the cerebellum, immunoreactivity was present in neurons of the deep cerebellar nuclei and in some neurons of the molecular and granular cell layers. Immunoreactivity was also present in endothelial cells of the subarachnoid vessels and those adjacent to ventricles in the cortex. The presence of well defined cytoplasmic aromatase immunoreactivity in neurons, some astrocytes, and endothelial cells suggests estrogen involvement in CNS physiology and function in the dog. The presence of aromatase in ependymal cells lining cerebral ventricles and choroid epithelial cells suggests that these cells may be partially responsible for estrogen concentration in the cerebrospinal fluid.

Investigation of diagnostic use of serum anti-Müllerian hormone concentration in dioestrus and anoestrus bitches before and after ovariohysterectomy and the relationship with ovarian follicle numbers

75%

Anadol E. , Gultiken N. , Yarim G. F. , Karaca E. , Kanca H. , Yarim M.

tom 23

nr 3

Investigation of diagnostic use of serum anti-Müllerian hormone concentration in dioestrus and anoestrus bitches before and after ovariohysterectomy and therelationship with ovarian follicle numbers

71%

Anadol E. , Gultiken N. , Yarim G. F. , Karaca E. , Kanca H. , Yarim M. , Anadol E. , Gultiken N. , Yarim G. F. , Karaca E. , Kanca H. , Yarim M.

nr 3